Abstract
Anther culture is the most used technique to produce doubled haploid lines in rice. This technique is well developed in a wide range of indica rice genotypes. However, in japonica type, and more specifically, the Mediterranean japonica, the protocols are yet to be optimized. Japonica and indica have different androgenic response, as well as different induction and regeneration rates, albinism ratios and chromosome doubling competence. The step-by-step anther culture protocol presented in this chapter allows to regenerate doubled haploid rice plantlets from anther microspores in 8 months. We also include an in vitro chromosome doubling protocol to induce doubled haploids from haploid plantlets by immersion in a colchicine solution. This chromosome doubling protocol complements the anther culture by taking advantage of the regenerated haploid plantlets.
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References
Niizeki H, Oono K (1968) Induction of haploid rice plant from anther culture. Proc Jpn Acad 44:554–557
Seguí-Simarro JM (2010) Androgenesis revisited. Bot Rev 76(3):377–404
Rukmini M, Rao GJN, Rao RN (2013) Effect of cold pretreatment and phytohormones on anther culture efficiency of two Indica rice (Oryza sativa L). Exp Biol Agr Sci 2:69–76
Hooghvorst I, Ramos-Fuentes E, López-Cristofannini C, Ortega M, Vidal R, Serrat X, Nogués S (2018) Antimitotic and hormone effects on green double haploid plant production through anther culture of Mediterranean japonica rice. Plant Cell Tissue Organ Cult 134(2):205–215
Lentini Z, Roca WM, Martinez CP (1997) Cultivo de anteras de arroz en el desarrollo de germoplasma, vol 293. CIAT
He T, Yang Y, Tu SB, Yu MQ, Li XF (2006) Selection of interspecific hybrids for anther culture of indica rice. Plant Cell Tissue Organ Cult 86(2):271–277
Chen C-C, Tsay H-S, Huang C-R (1991) Factors affecting androgenesis in rice (Oryza sativa L.). In: Bajaj YPS (ed) Rice. Springer, Berlin, pp 193–215
Kaushal L, Balachandran SM, Ulaganathan K, Shenoy V (2014) Effect of culture media on improving anther culture response of rice (Oryza sativa L.). Int J Agric Innov Res 3(1):218–224
Chu CC (1978) The N6 medium and its application to anther culture of cereal crops. Symp. Plant Tiss. Cult. Science Press, Beijing
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473–497
Ball ST, Zhou H, Konzak CF (1993) Influence of 2, 4-D, IAA, and duration of callus induction in anther cultures of spring wheat. Plant Sci 90(2):195–200
Trejo-Tapia G, Maldonado Amaya U, Salcedo Morales G, De Jesús Sánchez A, Martínez Bonfil B, Rodríguez-Monroy M et al (2002) The effects of cold-pretreatment, auxins and carbon source on anther culture of rice. Plant Cell Tissue Organ Cult 71(1):41–46
Powell W (1988) The influence of genotype and temperature pre-treatment on anther culture response in barley (Hordeum vulgare L.). Plant Cell Tissue Organ Cult 12(3):291–297
Yoshida KT, Fujii S, Sakata M, Takeda G (1994) Control of organogenesis and embryogenesis in rice calli. Jpn J Breed 44(4):355–360
Alemanno L, Guiderdoni E (1994) Increased doubled haploid plant regeneration from rice (Oryza sativa L.) anthers cultured on colchicine-supplemented media. Plant Cell Rep 13(8):432–436
Hooghvorst I, Ribas P, Nogués S (2020) Chromosome doubling of androgenic haploid plantlets of rice (Oryza sativa) using antimitotic compounds. Plant Breed 139(4):754–761. https://doi.org/10.1111/pbr.12824
Chu C-C (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sinica 18:659–668
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d’Hooghvorst, I., Ferreres, I., Nogués, S. (2021). Anther Culture and Chromosome Doubling in Mediterranean Japonica Rice. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_19
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DOI: https://doi.org/10.1007/978-1-0716-1315-3_19
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