Abstract
The use of doubled haploid (DH) plants in plant breeding programmes is the fastest route to release new varieties (4–6 years), allowing for a rapid response to end-user needs. Microspore embryogenesis is one of the most efficient methods for DH plant production in bread wheat. In this process, microspores triggered by a stress treatment or by application of bioactive compounds are reprogrammed to follow an embryogenic pathway that leads to the production of haploid or DH plants. In this chapter, we describe a protocol for anther culture of bread wheat. This protocol is based on an osmotic and starvation treatment of the anthers followed by the application of a microtubule disrupting agent. Anthers are cultured in an ovary pre-conditioned medium with mature ovaries from cv. Caramba. This protocol has been applied to a wide range of genotypes and F1s from bread and spelt wheat.
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Acknowledgements
This research was supported by Projects AGL2013-46698-R and AGL2016-77211-R from State R&D Program Oriented to the Challenges of the Spanish Society, and funds for Reference Research Groups recognized by Diputación General de Aragón, Spain (Groups A06 and A08-20R: Genetics, Genomics, Biotechnology and Plant Breeding). Isabel Valero-Rubira was funded by the Spanish Ministry of Science and Innovation grant no. BES-2017-080970 (linked to Project AGL2016-77211-R).
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Castillo, A.M., Valero-Rubira, I., Allué, S., Costar, M.A., Vallés, M.P. (2021). Bread Wheat Doubled Haploid Production by Anther Culture. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_11
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DOI: https://doi.org/10.1007/978-1-0716-1315-3_11
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