Abstract
Mature coconut embryos were germinated in a modified Murashige and Skoog medium and then cultured on BMY3 medium incorporating sucrose in the range of 4 to 8%. α-Naphthaleneacetic acid (NAA) was added into the medium at concentrations ranging from 0 to 800 μM for periods of 4 to 24 weeks. Application of NAA for 4 weeks stimulated shoot growth, whereas application periods greater than 4 weeks had no significant effect. NAA in the range of 100–300 μM stimulated elongation of the primary root and the optimum concentration increased with increases in sucrose levels. Production of adventitious roots was stimulated by the addition of NAA with levels of 200 μM and above being the most effective. Increasing the sucrose concentration from 4% through to 8% stimulated root elongation in the absence of NAA and inhibited shoot growth whether NAA was present or absent.
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Abbreviations
- IAA:
-
indole-acetic acid
- NAA:
-
α-naphthaleneacetic acid
References
Ashburner GR & Thompson WK (1993) Coconut embryo culture for the international transfer of germplasm. In Proceedings of the International Symposium on Coconut Research and Development II. CPCRI, Kasaragod, India (in press)
Ashburner GR, Thompson WK, Maheswaran G & Burch JM (1991) The effect of solid and liquid phase in the basal medium of coconut (Cocos nucifera L.) embryo cultures. Oléagineux 46: 149–152
Assy Bah B, Durand-Gasselin T & Pannetier C (1987) Use of zygotic embryo culture to collect germplasm of coconut (Cocos nucifera L.). FAO/IBPGR Plant Genet. Resources Newsl. 71: 4–10
Assy Bah B, Durand-Gasselin T, Engelmann F & Pannetier C (1989) Culture in vitro d'embryons zygotiques de cocotier (Cocos nucifera L.). Méthode, révisée et simplifiée, d'obtention de plants de cocotiers transférables au champ. Oléagineux 44: 515–523
Borchert R (1973) Simulation of rhythmic tree growth under constant conditions. Physiol. Plant. 29: 173–180
Buffard-Morel J (1968) Recherches sur la culture in vitro des embryons de palmier à huile (Elaeis guineensis Jacq. var. Dura) V. Effets du glucose, du lévulose, du maltose et du saccharose. Oléagineux 23: 7–11
Davies WJ, Metcalfe J, Lodge TA & Costa AR da (1986) Plant growth substances and the regulation of growth under drought. Aust. J. Plant Physiol. 13: 105–125
Davis PB (1968) A study on the respiratory organs of Cocos nucifera Linn. Ceylon Coconut Quart. 3: 116–136
Dunlap JR, Kresovich S, & McGee RE (1986) The effect of salt concentration on auxin stability in culture medium. Plant Physiol. 81: 934–936
Eeuwens CJ (1978) Effects of organic nutrients and hormones on growth and development of tissue explants from coconut (Cocos nucifera) and date (Phoenix dactylifera) palms cultured in vitro. Physiol. Plant. 42: 173–178
Fulford RM, Passey AJ & Fitzgerald JD (1979) Vegetative propagation of coconuts by tissue culture. Rep. East Malling Res. Sta., 1978: 178
Fulford RM, Passey AJ & Justin SHFW (1981) Coconut propagation in vitro. Rep. East Malling Res. Sta., 1980: 145–146
Gupta PK, Kendurkar SV, Kulkarni VM, Shirgurka MV & Mascarenhas AF (1984) Somatic embryogenesis and plants from zygotic embryos of coconut (Cocos nucifera L.) in vitro. Plant Cell Rep. 3: 222–225.
Guzman EV de & Manuel GC (1977) Improved root growth in embryo and seedling cultures of coconut makapuno by the incorporation of charcoal in the growth medium. Philipp. J. Coconut Stud. 2: 35–39
Guzman EV de & Rosario DA del (1964) The growth and development of Cocos nucifera L. ‘makapuno’ embryo in vitro. Philipp. Agr. 48: 82–94
Guzman EV de, Rosario AG del & Eusebio EC (1971) The growth and development of coconut ‘makapuno’ embryo in vitro III. Resumption of root growth in high sugar media. Philipp. Agr. 53: 566–579
Justin SHFW & Fulford RM (1980) Vegetative propagation of coconuts by tissue culture. Rep. East Malling Res. Sta., 1979: 185
Karunaratne S, Kurakulaarachchi C & Gamage C (1985). A report on the culture of embryos of dwarf coconut, Cocos nucifera L. var nana in vitro. Cocos 3: 1–8
Karunaratne S, Santha S & Kovoor A (1991) An in vitro assay for drought-tolerant coconut germplasm. Euphytica 53: 25–30
Morel G & Wetmore RM (1951) Fern callus tissue culture. Amer. J. Bot. 38: 81–118
Murashige T & Skoog F (1962) A revised medium for the rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Ray PM (1958) Destruction of auxin. Annu. Rev. Plant Physiol. 9: 81–118
Rillo EP, Paloma MBF, Rodriguez BJ & Ignacio MTR (1988) Screening of coconut populations for resistance to Cadang-Cadang disease using in vitro cultured embryos. Philippines Coconut Authority, Albay Research Centre, Annu. Rep., 1988: 39–40
Rosario AG del & Guzman EV de (1976) The growth of coconut ‘makapuno’ embryos in vitro as affected by mineral composition and sugar level of the medium during the liquid and solid cultures. Philipp. J. Sci. 105: 215–22
Sajise SU & Guzman EV de (1972) Formation of adventitious roots in coconut ‘makapuno’ seedlings grown in medium supplemented with naphthalene acetic acid. Philipp. J Biol. 1: 197–206
Tomlinson PB (1990) The Structural Biology of Palms. Oxford University Press, Oxford
Toruan N (1978) Pertumbuhan dan perkembangan embrio kelapa dalam Kulturaseptik. Menara Perkebunan 46: 213–6
Turner NC & Begg JE (1981) Plant-water relations and adaptation to stress. Plant Soil 58: 97–131
Weatherhead MA, Burdon J & Henshaw GG (1978) Some effects of activated charcoal as an additive to plant tissue culture media. Z. Pflanzenphysiol. 89: 141–7
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Ashburner, G.R., Thompson, W.K. & Burch, J.M. Effect of α-naphthaleneacetic acid and sucrose levels on the development of cultured embryos of coconut. Plant Cell Tiss Organ Cult 35, 157–163 (1993). https://doi.org/10.1007/BF00032965
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DOI: https://doi.org/10.1007/BF00032965