Skip to main content
SpringerLink
Log in

Brassinosteroid-induced rice lamina joint inclination and its relation to indole-3-acetic acid and ethylene

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Brassinosteroid (BR)-induced rice (Oriza sativa L.) lamina joint (RLJ) inclination and its relationship to indole-3-acetic acid (IAA) and ethylene were investigated using BR isolated from beeswax. The effect of BR on RLJ inclination was time- and concentration-dependent. Etiolated lamina were more sensitive to BR than green lamina. The BR-induced inclination was accompanied by increased lamina fresh weight, total water content, free-water content, proton extrusion and ethylene production, and decreased bound-water content. Lamina dry weight was not changed. The inclination was due to greater expansion of the adaxial cells relative to the dorsal cells in the lamina joint. This response was caused by BR and/or BR-induced signal(s) that were transported from the leaf sheath to the leaf blade. Both BR-induced RLJ inclination and ethylene production were inhibited by cobalt chloride (CoCl2), an inhibitor of ACC oxidase. BR-induced inclination was much higher than that of IAA, and was inhibited by high concentration of 2,3,5-triiodobenzoic acid (TIBA), an inhibitor of IAA transport. A synergistic effect was observed between BR and IAA. These results suggest that the effects of BR on RLJ inclination and pulvinus cell expansion may be resulted from BR-increased water potential and proton extrusion in the lamina. The BR-induced RLJ inclination may involve the action of ethylene but may be independent of IAA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BR:

brassinolide or brassinosteroid(s)

IAA:

indole-3-acetic acid

TIBA:

2,3,5-triiodobenzoic acid

RLJ:

rice lamina joint

References

  1. Abe H (1991) Rice-lamina inclination, endogenous levels in plant tissues and accumulation during pollen development of brassinosteroids. In: Cutler HG, Yokota T and Adam G (eds) Brassinosteroids-Chemistry, Bioactivity, and Applications, ACA Symposium Series 474, pp 26–35. Washington, DC: American Chemical Society

    Google Scholar 

  2. Abe H, Morishita T, Uchiyama M, Taka (1983) Occurrence of three new brassinosteroids: brassinone, (24S)-24-ethylbrassinone and 28-norbrassinolide in higher plants. Experientia 39: 351–353

    Google Scholar 

  3. Arteca RN, Bachman JM and Mandava NB (1988) Effects of indole-3-acetic acid and brassinosteroid on ethylene biosynthesis in etiolated mung bean hypocotyl segments. J Plant Physiol 133: 430–435

    Google Scholar 

  4. Baba J, Yokota T and Takahashi N (1983) Brassinolide-related new bioactive steroids from Dolichos lablab seed. Agric Biol Chem 47: 659–661

    Google Scholar 

  5. Cerana R, Bonetti A, Marre MT, Romani G, Lado P and Marre E (1983) Effects of a brassinosteroid on growth and electrogenic proton extrusion in Azuki bean epicotyls. Physiol Plant 59: 23–27

    Google Scholar 

  6. Clouse SD, Zurek DM, McMorris TC and Baker ME (1992) Effect of brassinolide on gene expression in elongating soybean epicotyls. Plant Physiol 100: 1377–1383

    Google Scholar 

  7. Cutler HG, Yokota T and Adam G (eds) (1991) Brassinosteroids-Chemistry, Bioactivity, and Applications, ACS Symposium series 474, Am Chem Soc, Washington, DC. 358 p

    Google Scholar 

  8. Grove MD, Spencer FG, Rohwedder WK, Mandava NB and Worley JF (1979) A unique plant growth promoting steroid from Brassica napus pollen. Nature 281: 216–217

    Google Scholar 

  9. Jiang J, Chen S, Liu G, Pan X and Cao H (1987) Study of plant hormones in beeswax-brassinosteroids. Chinese Sci Bull 28: 60–63

    Google Scholar 

  10. Kim SK (1991) Natural occurrences of brassinosteroids. In Cutler HG, Yokota T and Adam G (eds) Brassinosteroid-Chemistry, Bioactivity, and Applications, ACA Symposium Series 474, pp 26–35. Washington, DC: American Chemical Society

    Google Scholar 

  11. Kim SK, Abe H, Little CHA and Pharis RP (1990) Identification of two brassinosteroids from the cambial region of Scots Pine (Pinus silverstris) by gas chromatography-mass spectrometry, after detection using a dwarf rice lamina inclination bioassay. Plant Physiol 94: 1709–1713

    Google Scholar 

  12. Mandava NB (1988) Plant growth-promoting brassinoteroids. Annu Rev Plant Physiol Plant Mol Biol 39: 23–52

    Article  Google Scholar 

  13. Mitchell JW, Mandava NB, Worley JF, Plimmer JR and Smith MV (1970) Brassins: a new family of plant hormones from rape pollen. Nature 225: 1065–1066

    Google Scholar 

  14. Romani G, Marre MT, Bonetti A, Cerana R, Lado P and Marre E (1983) Effects of brassinosteroid on growth and electrogenic proton extrusion in maize root segments. Physiol Plant 59: 528–532

    Google Scholar 

  15. Schlagnhaufer C and Arteca RN (1985) Brassinosteroid-induced epinasty in tomato plants. Plant Physiol 78: 300–303

    Google Scholar 

  16. Shanghai Society of Plant Physiology (1985) Handbook of Plant Physiology Experiments. Shanghai: Science and Technology Press, pp 403–410

    Google Scholar 

  17. Takatsuto S, Yazawa N, Ikekawa N, Takematsu T, Takeuchi Y and Koguchi M (1983) Structure-activity relationship of brassinosteroids. Phytochemistry 22: 2437–2441

    Article  Google Scholar 

  18. Takeno K and Pharis RP (1982) Brassinosteroid-induced bending of the leaf lamina of dwarf rice seedlings: an auxinmediated phenomenon. Plant Cell Physiol 23: 1275–1281

    Google Scholar 

  19. Wada K and Marumo S (1981) Synthesis and plant growth-promoting activity of brassinolide analogues. Agric Biol Chem 45: 2579–2585

    Google Scholar 

  20. Wada K, Marumo S, Abe H, Morishita T and Nakamura K (1984) A rice lamina inclination test-a micro-quantitative bioassay for brassinosteroids. Agric Biol Chem 48: 719–726

    Google Scholar 

  21. Wada K, Marumo S, Ikekawa N, Morisaki M and Mori K (1981) Brassinolide and homobrassinolide promotion of lamina inclination of rice seedlings. Plant Cell Physiol 22: 323–325

    Google Scholar 

  22. Wada K, Marumo S, Ikekawa N, Morisaki M and Mori K (1983) The rice lamina inclination-promoting activity of synthetic brassinolide analogues with a modified side chain. Agric Biol Chem 47: 1139–1141

    Google Scholar 

  23. Wang TW, Cosgrove DJ and Arteca RN (1993) Brassinosteroid stimulation of hypocotyl elongation and wall relaxation in Pakchoi (Brassica chinensis cv Lei-Choi). Plant Physiol 101: 965–968

    PubMed  Google Scholar 

  24. Yokota T, Higuchi K, Kosaka Y and Takahashi N (1992) Transport and metabolism of brassinosteroid in rice. In: Karssen CM, vanLoon LC and Vreugdenhil D (eds) Progress in Plant Growth Regulation, pp 298–305. The Netherlands: Kluwer Academic Publishers

    Google Scholar 

  25. Yokota T, Baba J and Takahashi N (1983) Brassinolide-related bioactive sterols in Dolichos lablab: brassinolide, castasterone and an analog, homodolicholide. Agric Biol Chem 47: 1409–1411

    Google Scholar 

Download references

Author information

Author notes

  1. Heping Cao

    Present address: Department of Biochemistry, Michigan State University, 48824, East Lansing, MI, USA

Authors and Affiliations

  1. Department of Agronomy, Jiangxi Agricultural University, 330045, Nanchang, Jiangxi Province, The People's Republic of China

    Heping Cao & Shankun Chen

Authors
  1. Heping Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shankun Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cao, H., Chen, S. Brassinosteroid-induced rice lamina joint inclination and its relation to indole-3-acetic acid and ethylene. Plant Growth Regul 16, 189–196 (1995). https://doi.org/10.1007/BF00029540

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00029540

Key words

Search

Navigation