Skip to main content
SpringerLink
Log in

Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

Mitochondrial DNA (mtDNA) from the cryptomonad Pyrenomonas salina was isolated by CsCl-buoyant density centrifugation of whole-cell DNA in the presence of Hoechst dye 33258. mtDNA consists of circular molecules about 47 kb in size as estimated from restriction enzyme analysis. A physical map for six restriction enzymes (Bam HI, Bge I, Eco RI, Pst I, Sac I and Sac I) has been constructed. Genes coding for the small subunit of rRNA, cytochrome oxidase subunits I and II, and apocytochrome b were localized on this map using Southern blot hybridization with heterologous gene probes from Oenothera. Genes for 5S rRNA and NADH dehydrogenase subunit 5 are absent from P. salina mtDNA. The mitochondrial genome, being the first analysed to this extent in chromophytic algae, should be valuable for taxonomic and phylogenetic studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aldrich J, Cattolico RA: Isolation and characterization of chloroplast DNA from the marine chromophyte, Olisthodiscus luteus; electron microscopic visualization of isomeric molecular forms. Plant Physiol 68: 641–647 (1981).

    Google Scholar 

  2. Alt J, Westhoff P, Herrmann RG: Nucleotide sequence of the clustered genes for the 44 kd chlorophyll a apoprotein and the ‘32 kd’-like protein of the photosystem II reaction center in the spinach plastid chromosome. Curr Gener 8: 597–606 (1984).

    Google Scholar 

  3. Bayen M, Rode A: The 1.700 DNA of Chlorella pyrenoidosa: heterogeneity and complexity. Plant Sci Lett 1: 385–389 (1973).

    Article  Google Scholar 

  4. Bibb MJ, Van Etten RA, Wright CT, Walberg MW, Clayton DA: Sequence and gene organisation of the mouse mitochondrial DNA. Cell 26: 167–180 (1981).

    Article  PubMed  Google Scholar 

  5. Boer PH, Gray MW: The URF 5 gene of Chlamydomonas reinhardtii mitochondria: DNA sequence and mode of transcription. EMBO J 5: 21–28 (1986).

    PubMed  Google Scholar 

  6. Chomyn A, Mariottini P, Cleeter MW, Ragan CI, Matsuno-Yagi A, Hatefi Y, Doolittle RF, Attardi G: Six unidentified reading frames of human mitochondrial DNA encode components of the respiratory chain NADH dehydrogenase. Nature 314: 592–597 (1985).

    PubMed  Google Scholar 

  7. Clark-Walker GD, McArthur CR, Sriprakash KS: Location of transcriptional control signals and transfer RNA sequences in Torulopsis glabrata mitochondrial DNA. EMBO J 4: 465–473 (1985).

    PubMed  Google Scholar 

  8. Clark-Walker GD, Sriprakash KS: Size diversity and sequence rearrangements in mitochondrial DNAs from yeasts. In: Slonimski P, Borst P, Attardi G (eds) Mitochondrial Genes, pp. 349–354. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).

    Google Scholar 

  9. Clary DO, Wahleithner JA, Wolstenholme DR: Sequence and arrangement of the genes for cytochrome b, URF1, URF4L, URF4, URF5, URF6 and five tRNAs in Drosophila mitochondrial DNA. Nucl Acids Res 12: 3747–3762 (1984).

    PubMed  Google Scholar 

  10. de Bruijn MHL: Drosophila melanogaster mitochondrial DNA, a novel organisation and genetic code. Nature 304: 234–241 (1983).

    PubMed  Google Scholar 

  11. de la Cruz VF, Neckelmann N, Simpson L: Sequences of six genes and several open reading frames in the kinetoplast maxicircle DNA of Leishmania tarentolae, J Biol Chem 259: 15136–15147 (1984).

    PubMed  Google Scholar 

  12. Douglas SE, Durnford DG: The small subunit of ribulose-1, 5-bisphosphate carboxylase is plastid encoded in the chlorophyll c-containing alga Cryptomonas Φ. Plant Mol Biol 13: 13–20 (1989).

    PubMed  Google Scholar 

  13. Dujon B: Mitochondrial genes, mutants and maps: A review. In: Schwegen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983, pp. 1–24. De Gruyter, Berlin (1983).

    Google Scholar 

  14. Feinberg AP, Vogelstein B: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).

    PubMed  Google Scholar 

  15. Fonty G, Crouse E, Stutz C, Bernardi G: The mitochondrial genome of Euglena gracilis. Eur J Biochem 54: 367–372 (1975).

    PubMed  Google Scholar 

  16. Gebeyehu G, Rao PY, SooChan P, Simms DA, Klevan L: Novel biotinylated nucleotide-analogs for labeling and colorimetric detection of DNA. Nucl Acids Res 15: 4513–4534 (1987).

    PubMed  Google Scholar 

  17. Gibbs SP: Nuclear envelope-chloroplast relationship in algae. J Cell Biol 14: 433–444 (1962).

    Article  PubMed  Google Scholar 

  18. Gibbs SP: The chloroplast of some algal groups may have evolved from endosymbiotic eukaryotic algae. In: Fredrick JF (ed) Origins and Evolution of Eukaryotic Intracellular Organelles, Ann New York Acad Sci 361: 193–208 (1981).

  19. Gillot MA, Gibbs SP: The cryptomonad nucleomorph: its ultrastructure and evolutionary significance. J Phycol 16: 558–568 (1980).

    Google Scholar 

  20. Goff LJ: The biology of parasitic red algae. Progr Phycol Res 1: 289–369 (1982).

    Google Scholar 

  21. Grivell LA: Mitochondrial gene expression 1983. In: Schwegen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983, pp. 25–45. De Gruyter, Berlin (1983).

    Google Scholar 

  22. Guillard RRL. Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH (eds) Culture of Marine Invertebrates Animals. pp. 29–60, Plenum Press, New York (1975).

    Google Scholar 

  23. Hansmann P, Maerz M, Sitte P: Investigations on genomes and nucleic acids in Cryptomonads. Endocyt Cell Res 4: 289–295 (1987).

    Google Scholar 

  24. Herrmann RG, Whitfeld PR, Bottomley W: Construction of a SalI/PstI restriction map of spinach chloroplast DNA using low-gelling-temperature agarose electrophoresis. Gene 8: 179–191 (1980).

    Article  PubMed  Google Scholar 

  25. Hiesel R, Brennicke A: Cytochrome oxidase subunit II gene in mitochondria of Oenthera has no intron. EMBO J 2: 2173–2178 (1983).

    Google Scholar 

  26. Hiesel R, Schobel W, Schuster W, Brennicke A: The cytochrome oxidase subunit I and subunit III genes in Oenothera mitochondria are transcribed from identical promotor sequences. EMBO J 6: 29–34 (1987).

    Google Scholar 

  27. Hintz WE, Mohan M, Anderson JB, Horgen PA: The mitochondrial DNAs of Agaricus: heterogeneity in A. bitorguis and homogeneity in A. brunnescens. Curr Genet 9: 127–132 (1985).

    Google Scholar 

  28. Ise W, Haiker H, Weiss H: Mitochondrial translation of subunits of the rotenone-sensitive NADH: ubiquinone reductase in Neurospora crassa. Embo J 4: 2075–2080 (1985).

    PubMed  Google Scholar 

  29. Lang BF, Ahne F, Distler S, Trinkl H, Kaudewitz F: Sequence of the mitochondrial DNA, arrangement of genes, and processing of their transcripts in Schizo-saccharomyces pombe. In: Schwegen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983, pp. 313–329. De Gruyter, Berlin (1983).

    Google Scholar 

  30. Lee RE, Kugrens P. Leucocryptos, a colourless cryptophyte with the feeding apparatus of a suctorian ciliate. J Phycol 25 (Suppl): 5 (1989).

    Google Scholar 

  31. Li N, Cattolico RA: Chloroplast genome characterization in the red alga Griffithsia pacifica. Mol Gen Genet 209: 343–351 (1987).

    Article  Google Scholar 

  32. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).

    Google Scholar 

  33. Moore LJ, Coleman AW: The linear 20 kb mitochondrial genome of Pandorina morum (Volvocaceae, Chlorophyta). Plant Mol Biol 13: 459–465 (1989).

    Article  PubMed  Google Scholar 

  34. Perasso R, Baroin A, Qu LH, Bachellerie JP, Adoutte A: Origin of the algae. Nature 339: 142–144 (1989).

    Article  PubMed  Google Scholar 

  35. Roberts KR, Stewart KD, Mattox KR: The flagellar apparatus of Chilomonas paramecium (Cryptophyceae) and its comparison with certain zooflagellates. J Phycol 17: 159–167 (1981).

    Google Scholar 

  36. Ryan R, Grant D, Chiang KS, Swift H: Isolation and characterization of chloroplast DNA from Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 75: 3268–3272 (1978).

    PubMed  Google Scholar 

  37. Santore UJ, Greenwood AD: The mitochondrial complex in Cryptophyceae. Arch Microbiol 112: 207–218 (1977).

    PubMed  Google Scholar 

  38. Schuster W, Brennicke A: TGA termination in the apocytochrome b gene of Oenothera. Curr Genet 9: 157–163 (1985).

    Google Scholar 

  39. Sederoff RR: Structural variation in mitochondrial DNA. Adv Genet 22: 1–108 (1984).

    Google Scholar 

  40. Sitte P, Baltes S: Morphometric analysis of two cryptomonads. Quantitative evaluation of fine-structural changes in an endosymbiotic system. In: Nardon P, Gianinazzi-Pearson V, Grenier AM, Margulis L, Smith DC: Endocytobiologie IV, pp. 229–233. INRA, Paris (1990).

    Google Scholar 

  41. Sitte P: Zellen in Zellen: Endocytobiose und die Folgen. In: Verhandlungen der Gesellschaft Deutscher Naturforscher und Ärzte 114: 431–446 (1987).

  42. Spencer DF, Bonen L, Gray MW: Primary sequence of wheat mitochondrial 5S ribosomal ribonucleic acid: functional and evolutionary implications. Biochemistry 20: 4022–4029 (1981).

    PubMed  Google Scholar 

  43. Stern DB, Bang AG, Thompson WF: The watermelon URF 1 gene: evidence for a complex structure. Curr Genet 10: 857–869 (1986).

    PubMed  Google Scholar 

  44. Taylor FJR: Implications and extensions of the Serial Endosymbiosis Theory of the origin of eukaryotes. Taxon 23: 229–258 (1974).

    Google Scholar 

  45. Wallace DC: Structure and evolution of organelle genomes. Microbiol Rev 46: 208–240 (1982).

    PubMed  Google Scholar 

  46. Whatley JM, Whatley FR: Chloroplast evolution. New Phytol 87: 223–247 (1981).

    Google Scholar 

  47. Wissinger B, Hiesel R, Schuster W, Brennicke A: The NADH-dehydrogenase subunit 5 gene in Oenothera mitochondria contains two introns and is co-transcribed with the 5S rRNA gene. Mol Gen Genet 212: 56–65 (1988).

    Article  PubMed  Google Scholar 

  48. Wolters J, Erdmann VA: Cladistic analysis of 5S rRNA and 16S rRNA secondary and primary structure-The evolution of eukaryotes and their relation to Archaebacteria. J Mol Evol 24: 152–166 (1986).

    Google Scholar 

  49. Zurawski G, Perrot B, Whitfeld PR, Bottomley W: The structure of the gene for the large subunit of ribulose-1, 5-bisphosphate carboxylase from spinach chloroplast DNA. Nucl Acids Res 9: 3251–3270 (1981).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Lehrstuhl für Zellbiologie, Institut für Biologie II, Schänzlestr. 1, D-7800, Freiburg, Germany

    Martina Maerz & Peter Sitte

Authors
  1. Martina Maerz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter Sitte
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Maerz, M., Sitte, P. Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina . Plant Mol Biol 16, 593–600 (1991). https://doi.org/10.1007/BF00023424

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00023424

Key words

Search

Navigation