Summary
In our previous report of a series of experiments involving ordinary grafting, virus-inoculated grafting and DNA treatment in Capsicum annuum L., we described the variants as well as gene analyses of individual variants, together with the characteristics of the graft-induced genetic changes obtained.
Microhistological analysis of the stock stems revealed that chromatin masses, stained to greenish blue and in various sizes and shapes, were moving through cell wall and intercellular space from the lignifying and dying cells, stained to lighter purplish brown, towards the vascular bundles (Fig. 1). I discussed the mechanisms of chromatin transfer from the stock dying cells through the vascular system across the graft-union to the growing point(s) of the scion (Fig. 3), and how that process causes transformation in the fast dividing scion flower primordia (Fig. 4).
The significance of chromatin translocation can be understood in the following way: Genetic information is never transmitted from the stock to the scion, unless a DNA molecule larger than a functional unit such as a gene translocates from the stock, and unless the DNA molecules per se are integrated into new cell nuclei of the scion. In other words, no transmission of genetic information will take place, if DNA molecules of the stock disintegrated to nucleotides, as when a protein molecule is digested into amino acids, and are utilized by the scion simply as raw material in the formation of new cell nuclei with its own DNA strand as template.
Also discussed were the significance of ‘mentor methods’, and the importance of distinguishing between horticultural graft and genetical graft (Fig. 2).
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References
Esau, K., 1948. Some anatomical aspects of plant virus disease problems. II. Bot. Rev. 14: 413–449.
Kasahara, J., T. Nakamura & Y. Yoneyama, 1973. Graft-induced variation in red peppers. (in Japanese) Rec. Adv. Breed. 13: 73–89.
Marmur, J., 1961. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3: 208–218.
Melchers, G., 1977. The combination of somatic and conventional genetics in plant breeding. Pl. Res. Devel. 5: 86–110.
Nawa, S., B. Sakaguchi, M.A. Yamada & M. Tsujita, 1971. Hereditary changes in Bombyx after treatment with DNA. Genetics 67: 221–234.
Nawa, S. & M.A. Yamada, 1967. Genetic effects of DNA in Ephestia. Natl. Inst. Genet. Japan Ann. Rep. 17: 48–49.
Nawa, S. & M.A. Yamada, 1968. Hereditary changes in Ephestia after treatment with DNA. Genetics 58: 573–584.
Nawa, S., M.A. Yamada & Y. Ohta, 1975. Hereditary changes in Capsicum annuum L. III. Induced by DNA treatment. Japan. J. Genet. 50: 341–344.
Ohta, Y., 1970. A variant found in the progeny from grafting in Capsicum annuum. Natl. Inst. Genet. Japan Ann. Rep. 20: 34–35.
Ohta, Y., 1977a. Hereditary changes induced by grafting and by DNA application and their implication in Capsicum breeding. In: E. Pochard (Ed), ‘Capsicum 77’, Proc. III Eucarpia Meeting on Genetics and Breeding of Capsicum, Avignon-Montfavet, France, pp. 147–153.
Ohta, Y., 1977b. Hereditary changes induced by grafting. (in Japanese) Sci. Amer. (Japanese ed.) 7 (7): 100–113.
Ohta, Y., 1977c. Graftoduction in Capsicum annuum L. and an interpretation on the mechanism. (in Japanese) Japan J. Breed. 27 (suppl. 2): 130–131.
Ohta, Y., 1986. High-efficiency genetic transformation of maize by a mixture of pollen and exogenous DNA. Proc. Natl. Acad. Sci. USA 83: 715–719.
Ohta, Y. & P.V. Chuong, 1975a. Hereditary changes in Capsicum annuum L. I. Induced by ordinary grafting. Euphytica 24: 355–368.
Ohta, Y. & P.V. Chuong, 1975b. Hereditary changes in Capsicum annuum L. II. Induced by virus-inoculated grafting. Euphytica 24: 605–611.
Pandey, K.K., 1976. Genetic transformation and ‘graft hybridization’ in flowering plants. Theor. Appl. Genet. 47: 299–302.
Pandey, K.K., 1985. Gene movement and genetic manipulation. In: M. Freeling (Ed), Plant Genetics, pp. 235–249. Alan R. Liss, Inc., New York.
Yagishita, N. & Y. Hirata, 1987. Graft-induced change in fruit shape in Capsicum annuum L. I. Genetic analysis by crossing. Euphytica 36: 809–814.
Yagishita, N., Y. Hirata, H. Mizukami, H. Ohashi & K. Yamashita, 1990. Genetic nature of low capsaicin content in the variant strains induced by grafting in Capsicum annuum L. Euphytica 46: 249–252.
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Ohta, Y. Graft-transformation, the mechanism for graft-induced genetic changes in higher plants. Euphytica 55, 91–99 (1991). https://doi.org/10.1007/BF00022565
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DOI: https://doi.org/10.1007/BF00022565