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The complete deduced amino acid sequences of legumin β-polypeptides from different genetic loci inPisum

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Summary

The deduced amino acid sequences of the β-polypeptides ofPisum legumin from two loci on chromosome 1 were compared with one from a locus on chromosome 7. The chromosome 1-derived sequences were ∼80% identical, but each was only ∼50% homologous to the chromosome 7-derived sequence. Comparison of these sequences with those of homologous polypeptides from two other species of the Leguminoseae showed that the chromosome 1-derivedPisum sequences were more similar to legumin B than to legumin A fromVicia faba and were more closely related to group II than to group I glycinins fromGlycine max. The converse was true for the chromosome 7-derivedPisum sequences. This suggests that divergence of legumin-like sequences predated speciation in these three members of the Leguminosease.

Among the threePisum sequence classes, a highly variable region was identified within the α-polypeptide, just to the amino-terminal side of the αβ processing site. This region varied considerably in length within the three classes ofPisum α-polypeptide sequence, a variation which far exceeded that which has previously been described for other legumins and glycinins. The chromosome 7-derived, and one of the chromosome 1-derived α-polypeptide sequences contained different tandem repeats in this region.

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Authors and Affiliations

  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, United Kingdom

    Claire Domoney & Rod Casey

  2. Plant Breeding Institute, Maris Lane, Trumpington, CB2 2LQ, Cambridge, United Kingdom

    Dick Barker

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  1. Claire Domoney
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  2. Dick Barker
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Domoney, C., Barker, D. & Casey, R. The complete deduced amino acid sequences of legumin β-polypeptides from different genetic loci inPisum . Plant Mol Biol 7, 467–474 (1986). https://doi.org/10.1007/BF00020330

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  • DOI: https://doi.org/10.1007/BF00020330

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