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Isolation and culture of protoplasts from the marine green algaMonostroma angicava

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Abstract

Protoplasts were isolated enzymatically from gametophytes of the marine green algaMonostroma angicava. The protoplasts regenerated in PES medium after gradual reduction of the osmoticum. Three types of developmental process were recognized in the protoplast regeneration: an original type, in which the protoplasts regenerated into leafy gametophytes; an apogamic type, in which they regenerated into sporophytes; a callus type, in which they regenerated into callus-like tissues. The resulting gametophytes and apogamic sporophytes became fertile in successive cultures.

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Authors and Affiliations

  1. Hokkaido Regional Fisheries Research Laboratory, Kushiro, Hokkaido, 085, Japan

    Naotsune Saga & Toshihiko Kudo

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  1. Naotsune Saga
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  2. Toshihiko Kudo
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Saga, N., Kudo, T. Isolation and culture of protoplasts from the marine green algaMonostroma angicava . J Appl Phycol 1, 25–30 (1989). https://doi.org/10.1007/BF00003532

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  • DOI: https://doi.org/10.1007/BF00003532

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