Abstract
An assessment was carried out to examine the TDZ (a potential cytokinin) efficacy in the induction of multiple shoot regeneration in Syzygium cumini from juvenile nodal (N) explants and cotyledonary node (CN) explants. TDZ in different concentration was used as a supplement to the MS basal media. Two factors viz. concentration and exposure duration to TDZ have multifaceted effect on multiple shoot induction and regeneration. Continuous presence of culture in TDZ inhibited shoot elongation and even leads to shoot fasciation. On transferring the TDZ exposed shoot clumps (4 weeks old) on hormone-free medium increased multiple shoot regeneration by threefold. Best TDZ concentration was recorded to be 5.0 μM in nodal explants while 2.5 in cotyledonary node explant. Maximum percentage response in CN explant was 75% with maximum mean shoot numbers (4.9 ± 0.67) and mean shoot length (2.90 ± 0.23 cm) while highest percent response was observed as 79% with maximum shoot numbers (4.17 ± 0.81) and maximum shoot length (2.87 ± 0.23 cm) in juvenile nodal (N) explants. The shoot clumps from CN (2.5 μM, TDZ) and juvenile nodal (5.0 μM, TDZ) when transferred onto secondary medium (hormone-free basal medium) produced mean shoot length (4.27 ± 0.34 cm) and (4.56 ± 0.35 cm) whereas mean shoot numbers noted to be (16.23 ± 0.21) and (18.34 ± 0.35), respectively. These shoot cultures are free from any discrepancy. Well-developed shootlet (3.0 cm) was successfully rooted in vitro in MS ½ and IBA with highest percent response in CN and juvenile nodal recorded to be 83% and 82% with mean root numbers (6.33 ± 0.10) and (5.99 ± 0.11) respectively and mean root length (7.19 ± 0.21 cm) and (6.79 ± 0.25 cm). The survival rate of transferred plant was nearly 70%.
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Naaz, A., Siddique, I., Ahmad, A. (2021). TDZ-Induced Efficient Micropropagation from Juvenile Nodal Segment of Syzygium cumini (Skill): A Recalcitrant Tree. In: Siddique, I. (eds) Propagation and Genetic Manipulation of Plants. Springer, Singapore. https://doi.org/10.1007/978-981-15-7736-9_12
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