Abstract
The effect of thidiazuron (TDZ) was investigated on in vitro shoot proliferation from nodal explants of Rauvolfia tetraphylla. Murashige and Skoog (MS) medium containing TDZ (0.5–10μM) was effective in inducing shoot buds and maintaining high rates of shoot multiplication on hormone free medium. The highest shoot regeneration frequency (90%) and mean number (18.50 ± 1.25) of shoots per explant were achieved from nodal segments cultured on MS medium supplemented with 5μM TDZ for 4 weeks prior to transfer to MS medium without TDZ for 8 weeks. The regenerated shoots rooted best on MS medium containing 0.5μM indole-3-butyric acid (IBA). Micropropagated plantlets were hardened to survive ex vitro conditions and were then established into soil.
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Abbreviations
- BA:
-
6-benzyladenine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- Kinetin:
-
6-furfurylaminopurine
- MS:
-
Murashige and Skoog medium
- NAA:
-
α-naphthalene acetic acid
- TDZ:
-
thidiazuron
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Faisal, M., Ahmad, N. & Anis, M. Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tiss Organ Cult 80, 187–190 (2005). https://doi.org/10.1007/s11240-004-0567-x
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DOI: https://doi.org/10.1007/s11240-004-0567-x