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Abstract

Somatic embryogenesis has been defined as the initiation of embryos from plant somatic tissues closely resembling their zygotic counterparts (Ammirato, 1983). This morphologic event has been documented as occurring spontaneously in some plant species (Yarbrough, 1932; Taylor, 1967), however it is usually induced in tissue cultures, potentially from almost any part of the plant body, directly from the explant (Dubois et al., 1991) or — more frequently — after a callus stage. In vitro somatic embryogenesis was first reported in 1958 (Reinert, 1958; Steward, 1958; Steward et al., 1958) in Daucus carota tissue cultures; since then, it has been described for many species, and proved to be widespread in the various plant taxa.

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References

  • Altamura, M.M., Cersosimo, A., Majoli, C., and M. Crespan (1992) Histological study of embryogenesis and organogenesis from anthers of Vitis rupestris du Lot cultured in vitro. Protoplasma 171: 134–141.

    Article  Google Scholar 

  • Altamura, M.M., Cersosimo A., Majoli C., Crespan M., and F. Capitani (1993) Formation of secondary embryoids obtained from anthers of Vitis rupestris Scheele cultured in vitro. Giorn. Bot. Ital. 127: 297–299.

    Google Scholar 

  • Ammirato, P.V. (1983) Embryogenesis. In: Techniques for Propagation and Breeding, Evans D.A., Sharp W.R., Ammirato P.V., Yamada Y. (Eds). Handbook of Plant Cell Cultures, Vol. I, pp. 82–123.

    Google Scholar 

  • Bouquet A., Piganeau, B., and M. Lamaison (1982) Influence du génotype sur la production de cals, d’embryoides et de plantes entières par culture d’anthères in vitro dans le genre Vitis. Compt. Rend. Acad. Sci. Paris 295: 569–574.

    Google Scholar 

  • Cao, Z. (1990) Grape: anther culture. In:: Handbook of Plant Cell Culture, Vol. VI, Perennial crops, Zenghua Chen et al. (Eds). McGraw-Hill, New York, pp. 300–311

    Google Scholar 

  • Chambers, S.A. and B.R. Shaw (1987) Histone modifications accompanying the onset of developmental commitment. Dev. Biol. 124: 523–531.

    Article  PubMed  CAS  Google Scholar 

  • Choi, J.H. and Z.R. Sung (1989) Induction, commitment and progression of plant embryogenesis. In: Plant Biotechnology, Kung S., Arntzen C.J. (Eds). Butterworths, pp. 141–159.

    Google Scholar 

  • Compton, M.E. and D.J. Gray (1996) Effects of sucrose and methylglyoxal bis-(guanylhydrazone) on controlling grape somatic embryogenesis. Vitis 35 (1): 1–6.

    CAS  Google Scholar 

  • Coutos-Thevenot, P., Goebel-Tourand, I., Mauro, M.C., Jouanneau, J.P., Boulay, M., Deloire, A., and J. Guern (1992a) Somatic embryogenesis from grapevine cells. I-Improvement of embryo development by changes in culture conditions. Plant Cell Tiss. Org. Cult. 29: 125–133.

    Article  Google Scholar 

  • Coutos-Thevenot, P., Maes, O., Jouenne, T., Mauro, M.C., Boulay, M., Deloire, A., and J. Guern (1992b) Extracellular protein patterns of grapevine cell suspensions in embryogenic and non-embryogenic situations. Plant Sci. 86: 137–145

    Article  CAS  Google Scholar 

  • De Vries, S.C. (1998) Making embryos in plants. Trends in Plant Sci. 3: 451–452

    Article  Google Scholar 

  • Deloire, A. and M.C. Mauro (1991) Amélioration de la vigne par la voie des biotechnologies: realités et perspectives, Rev. Cytol. Biol. Veget. Bot. 14: 265–269.

    Google Scholar 

  • Dos Santos, A.P., Cutter, E.G., and M.R. Davey (1983) Origin and development of somatic embryos in Medieago saliva L. (alfalfa). Protoplasma 117: 107–115.

    Article  Google Scholar 

  • Dubois, T., Guedrira, M., Dubois, J., and J. Vasseur (1991) Direct somatic embryogenesis in leaves of Cichorìum: a histological and SEM study of early stages. Protoplasma 162: 120–127.

    Article  Google Scholar 

  • Emershad, R.L. and D.W. Ramming (1994) Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (V. vinifera L.). Plant Cell Rep. 14: 6–12.

    Article  CAS  Google Scholar 

  • Eynard, I. and G. Dalmasso (1990) Viticultura Moderna (l0’ß edition). Hoepli Publ., Milano. Fallot, J., Tey-Rulh, P., Coutouly, P., Petitprez, M., Roustan, J.P., Philippe, I., and R. Tabacchi (1990) Cultures in vitro, étude de l’eutypiose et stratégies de création de somaclones de vigne tolérants. Les colloques de l’INRA 51: 151–159.

    Google Scholar 

  • Faure, O. (1990) Embryons somatiques de Vitis rupestris et embryons’zygotiques de Vitis sp: morphologie, histologie, histochimie et développement. Can. J. Bot. 68: 2305–2315.

    Google Scholar 

  • Faure, O. and J. Aarrouf (1994) Metabolism of reserve products during development of somatic embryos and germination of zygotic embryos in grapevine. Plant Sci. 96: 167–178.

    Article  CAS  Google Scholar 

  • Faure, O. and A. Nougarède (1993) Nuclear DNA content of somatic and zygotic embryos of V. vinifera cv Grenache noir at the torpedo stage. Flow cytometry and in situ DNA microspectrophotometry. Protoplasma 176: 145–150.

    Article  Google Scholar 

  • Faure, O., Aarrouf, J., and A. Nougarède (1996a) Ontogenesis, differentiation and precocious germination in anther-derived somatic embryos of grapevine (V. vinifera L.): proembryogenesis. Ann. Bot. 78: 23–8.

    Article  Google Scholar 

  • Faure, O., Aarrouf, J., and A. Nougarède (1996b) Ontogenesis, differentiation and precocious germination in anther-derived somatic embryos of grapevine (V. vinifera L.): embryonic organogenesis. Ann. Bot. 78: 29–37.

    Article  Google Scholar 

  • Faure, O., Dewitte, W., Nougarède, A., and H. VanOnckelen (1998) Precociously germinating somatic embryos of V. vinifera have lower ABA and IAA levels than their germinating zygotic counterparts. Physiol. Plant. 102: 591–595.

    Article  CAS  Google Scholar 

  • Faure, O., Mengoli, M., Nougaréde, A., and N. Bagni (1991) Polyamine pattern and biosynthesis in zygotic and somatic embryo stages of V vinifera. J. Plant Physiol. 138: 545–549.

    Article  CAS  Google Scholar 

  • Favre, J.M. (1977) Premiers résultats concernant l’obtenition in vitro de néoformations caulinaires chez la Vigne. Ann. Amel. Plantes 27: 151–169.

    CAS  Google Scholar 

  • Finkelstein, R.R. and M.L. Crouch (1984) Precociously germinating rapeseed embryos retain characteristics of embryogeny. Planta 162: 125–131.

    Article  CAS  Google Scholar 

  • Franks, T., He, D.G., and M. Thomas (1998) Regeneration of transgenic V. vinifera L. Sultana plants: genotypic and phenotypic analysis. Molec. Breed 4: 321–333.

    Article  CAS  Google Scholar 

  • Gianazza, E., De Ponti, P., Scienza, A., Villa, P., and L. Martinelli (1992) Monitoring by two-dimensional electrophoresis somatic embryogenesis in leaf and petiole explants from Vitis. Electrophoresis 13: 203–209.

    Article  PubMed  CAS  Google Scholar 

  • Goebel-Tourand, I., Mauro, M.C., Sossountazov, L., Miginiac, E., and A. Deloire (1993) Arrest of somatic embryo development in grapevine: histological characterization and the effect of ABA, BAP and zeatin in stimulating plantlet development. Plant Cell Tiss. Org. Cult. 33: 91–103.

    Article  CAS  Google Scholar 

  • Goussard, P.G. and J. Wiid (1992) The elimination of fanleaf virus from grapevines using in vitro somatic embryogenesis combined with heat therapy. S. Afr. J. Enol. Vitic. 13: 81–83.

    Google Scholar 

  • Goussard, P.G., Wiid, J., and G.G.F. Kasdorf (1991) The effect of in vitro somatic embryogenesis in eliminating fanleaf virus and leafroll associated viruses from grapevines. S. Afr. J. Enol. Vitic. 12: 77–81.

    Google Scholar 

  • Gray, D.J. (1987) Quiescence in monocotyledonous and dicotyledonous somatic embryos induced by dehydration. HortSci. 22 (5): 810–814.

    Google Scholar 

  • Gray, D.J. (1989) Effects of dehydration and exogenous growth regulators on dormancy, quiescence and germination of grape somatic embryos. In vitro Cell Devel. Biol. 5: 1173–1178.

    Google Scholar 

  • Gray, D.J. (1992) Somatic embryogenesis and plant regeneration from immature zygotic embryos of muscadine grapevine (Vitis rotundifolia) cultivars. Am. J. Bot. 79: 542–546.

    Article  Google Scholar 

  • Gray, D.J. (1995) Somatic embryogenesis in grape. In: Somatic Embryogenesis in Woody Plants, Jain S., Gupta P. and R. Newton (Eds). Kluwer Acad. Pubi., NL, Vol. 2, pp. 191–217.

    Google Scholar 

  • Gray, D.J. and M.E. Compton (1993) Grape somatic embryo dormancy and quiescence: potential of dehydrated synthetic seeds for germplasm conservation. In: Synseeds, Applications of Synthetic Seeds to Crop Improvement, Redenbaugh K. (Ed.). CRC Press Inc., pp. 367–379.

    Google Scholar 

  • Gray, D.J. and C.P. Meredith (1992) Grape. In: Biotechnology of Perennial Fruit Crops. Hammerschlag F.A., Litz R.E. (Eds). CAB Intern. pp. 229–262.

    Google Scholar 

  • Gray, D.J. and J.A. Mortensen (1987) Initiation and maintenance of long term somatic embryogenesis from anthers and ovaries of Vitis longii “Microsperma”. Plant Cell Tiss. Org. Cult. 9: 73–80.

    Article  Google Scholar 

  • Gresshoff, P.M. and C.H. Doy (1974) Derivation of a haploid cell line from V. vinifera and the importance of the stage of meiotic development of anthers for haploid culture of this and other genera. Z. Pflanzenphysiol. 73: 123–141.

    Google Scholar 

  • Guha-Mukherjee, S. (1999) The discovery of haploid production by anther culture. Turning point article. In vitro Cell. Dev. Biol. Plant 35: 357–360.

    Article  Google Scholar 

  • Harst, M. (1995) Development of a regeneration protocol for high frequency somatic embryogenesis from explants of grapevines (Vitis spp.).Vitis 34 (1): 27–29.

    Google Scholar 

  • Hébert-Soulé, D., Kikkert, J.R., and B.I. Reisch (1995) Phosphothricin stimulates somatic embryogenesis in grape (Vitis sp. L.). Plant Cell Rep. 14: 380–4.

    Article  Google Scholar 

  • Hirabayashi, T., Kozaki, I., and T. Akihama (1976) In vivo differentiation of shoots from anther callus in Vitis grape research. HortSci. 11:511–512.

    Google Scholar 

  • Jayasankar, S., Gray, D.J., and R.E. Litz (1999) High-efficiency somatic embryogenesis and plant regeneration from suspension cultures of grapevine. Plant Cell Rep. 18: 533–537.

    Article  CAS  Google Scholar 

  • Konar, R.N., Thomas, E., and H.E. Street (1972) Origin and structure of embryoids arising from epidermal cells of the stem of Ranunculus scleratus L. J. Cell Sci. 11: 77–93.

    PubMed  CAS  Google Scholar 

  • Krochko, J.E., Bantroch, D.J., Greenwood, J.S., and J.D. Bewley (1994) Seed storage proteins in developing somatic embryos in alfalfa: defects in accumulation compared to zygotic embryos. J. Exp. Bot. 45: 699–708.

    Article  CAS  Google Scholar 

  • Krul, W.R. (1985) In vitro propagation of vine. USA Patent No. 4,532,733, Aug. 6, 1985.

    Google Scholar 

  • Krul, W.R. and J.F. Worley (1977) Formation of adventitious embryos in callus cultures of “Seyval”, a French hybrid grape. J. Am. Soc. Hort. Sci. 102 (3): 360–363.

    Google Scholar 

  • Krul, E.R. and G.FI. Mowbray (1984) Grapes. In: Handbook of Plant Cell Cultures, Sharp W.R., Evans D., Ammirato P.V., Yamada Y. (Eds). Vol. Il, Crop Species, pp. 396–434.

    Google Scholar 

  • Kuksova, V.B., Piven, N.M., and Y.Y. Gleba (1997) Somaclonal variation and in vitro induced mutagenesis in grapevine. Plant Cell Tiss. Org. Cult. 49: 17–27.

    Article  CAS  Google Scholar 

  • Lebrun, L. and M. Branchard (1986) Embryogenèse “indefinie “ chez Vitis sp. cultivé in vitro. Proc. 3° Symp. Intern. sur Physiologic do la Vigne, 24–27 June 1986, Bordeaux, France, pp. 38–41.

    Google Scholar 

  • Maes, O., Coutos-Thevenot, P., Jouenne, T., Boulay, M., and J. Guern (1997) Influence of extracellular proteins, proteases and protease inhibitors on grapevine somatic embryogenesis. Plant Cell Tiss. Org. Cult. 50: 97–105.

    Article  CAS  Google Scholar 

  • Marchenko, A.O. (1991) Induction of embryogenesis in primary calluses from grape stems and leaves. Fiziologiya Rastenii 38 (3): 580–591.

    CAS  Google Scholar 

  • Margosan, D.A., Emershad, R.L. and D.W. Ramming (1994) Origin of somatic embryos from embryo rescue cultures of seedless grapes (V. vinifera L.). Proc. Intern. Symp. on Table Grape Production, 28–29 June 1994, Anaheim, California, pp. 133–135.

    Google Scholar 

  • Martinelli, L. (1997) Regeneration and Genetic Transformation in the Vitis Genus. Ph.D. thesis for the award of the degree of Doctor of Agricultural and Environmental Sciences at the Agricultural University of Wageningen, The Netherlands.

    Google Scholar 

  • Martinelli, L. and G. Mandolino (1994) Genetic transformation and regeneration of transgenic plants in grapevine (Vitis rupestris S.). Theor. Appl. Genet. 88: 621–628.

    Article  Google Scholar 

  • Martinelli, L. and G. Mandolino (2001) Genetic transformation in Vitis. In: Biotechnology in Agriculture and Forestry, Bajaj Y.P.S. (Ed.). Springer-Verlag, Berlin, Vol. 47, pp. 325–338.

    Google Scholar 

  • Martinelli, L., Bragagna, P., Poletti, V., and A. Scienza (1993a) Somatic embryogenesis from leaf- and petiole-derived callus of Vitis rupestris. Plant Cell Rep. 12: 207–210.

    Article  Google Scholar 

  • Martinelli, L., Scienza, A., Villa, P., De Ponti, P. and E. Gianazza (1993b) Enzyme markers for somatic embryogenesis in Vitis. J. Plant. Physiol. 141: 476–481.

    Article  CAS  Google Scholar 

  • Martinelli, L., Rugini, E., and F. Saccardo (1996) Genetic transformation for biotic stress resistance in horticul- tural plants. World Congress on in vitro Biology, San Francisco, CA, June 22–27, 1996.1n vitro 3: 69A.

    Google Scholar 

  • Martinelli, L., Buzkan, N., Minafra, A., Saldarelli, P., Costa, D., Poletti, V., Festi, S., Perl, A., and G.P. Martelli (2000) Genetic transformation of tobacco and grapevines for resistance to viruses related to the rugose wood disease complex. Acta Hortic. 528: 321–327.

    CAS  Google Scholar 

  • Matsuta, N. and T. Hirabayashi (1989) Embryogenic cell lines from somatic embryos of grape (V. vinifera L.) Plant Cell Rep. 7: 684–687.

    CAS  Google Scholar 

  • Mauro, M.C., Nef, C., and J. Fallot (1986) Stimulation of somatic embryogenesis and plant regeneration from anther culture of V. vinifera cv Cabernet-Sauvignon. Plant Cell Rep. 5: 377–380.

    Article  Google Scholar 

  • McWilliam, A.A, Smith, S.M. and H.E. Street (1974) The origin and development of embryoids in suspension culture of carrot (Daucus carota). Ann. Bot. 38: 243–250.

    Google Scholar 

  • Monette, P.L. (1988) Grapevine (V. vinifera L.). In: Biotechnology in Agriculture and Forestry, Bajaj Y.P.S. (Ed.). Vol. VI, Crops. Springer-Verlag, Berlin, Heidelberg, pp. 3–37.

    Google Scholar 

  • Mordhorst, A.P., Toonen, M.A.J., and S. de Vries (1997) Plant embryogenesis. Plant Sci. 16: 535–576

    Google Scholar 

  • Moriguchi, T., Kozaki, 1., Matsuta, N. and S. Yamaki (1988) Plant regeneration from grape callus stored under a combination of low temperature and silicone treatment. Plant Cell Tiss. Org. Cult. 15: 67–71.

    Google Scholar 

  • Mozsar, J. and S. Sule (1994) A rapid method for somatic embryogenesis and plant regeneration from cultured anthers of Vitis riparia. Vitis 33: 245–246.

    Google Scholar 

  • Mozsar, J. and O. Viczian (1996) Genotype effect on somatic embryogenesis and plant regeneration of Vitis spp. Vitis 35 (4): 155–157.

    Google Scholar 

  • Mullins, M.G. (1990) Applications of tissue culture to the genetic improvement of grapevine. Proc. 51h Intern. Symp. on Grape Breeding. 12–16 Sept. 1989, St. Martin/Pfalz, FRG, Vitis, Special Issue, ISSN 00427500, pp. 399–407.

    Google Scholar 

  • Mullins, M.G. and K. Rajasekaran (1980) Plantlets from cultured anthers of Vitis species and hybrids. Proc. 3° Int. Symp. on Grape Breeding, Davis, CA: 111–119.

    Google Scholar 

  • Mullins, M.G. and C. Srinivasan (1976) Somatic embryos and plantlets from an ancient clone of the grapevine (cv Cabernet-Sauvignon) by apomixis in vitro. J. Exp. Bot. 27 (100): 1022–1030.

    Article  Google Scholar 

  • Mullins, M.G., Tang, F.C.A., and D. Facciotti (1990) Agrobacterium-mediated genetic transformation of grapevines: transgenic plants of Vitis rupestris Scheele and buds of V. vinifera L. Bio/Technology 8: 1041–1045.

    Google Scholar 

  • Murashige, T. and F. Skoog (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.

    Article  CAS  Google Scholar 

  • Nakano, M., Sakakibara, T., Watanabe, Y., and M. Mii (1997) Establishment of embryogenesis cultures in several cultivars of V. vinifera and V. x labruscana. Vitis 36 (3): 141–145.

    Google Scholar 

  • Newton, D.J. and P.G. Goussard (1990) The ontogeny of somatic embryos from in vitro cultured grapevine anthers. S. Afr. J. Enol. Vitic. 11: 70–75.

    Google Scholar 

  • Nitsch, J.P. and C. Nitsch (1969) Haploid plants from pollen grains. Science 163: 85–87.

    Article  PubMed  CAS  Google Scholar 

  • Pearce, D., Pharis, R.P., Rajasekaran, K., and M.G. Mullins (1987) Effects of chilling and ABA on (1H)gibberellin A4 metabolism in somatic embryos of grape (V. vinifera L. x V. rupestris Scheele). Plant Physiol. 80: 381–385.

    Article  Google Scholar 

  • Perl, A. and Y. Eshdat (1998) DNA transfer and gene expression in transgenic grapes. In: Biotechnology and Genetic Engineering Reviews, Thombs M.P. (Ed.). Vol. 15, pp. 365–386.

    Google Scholar 

  • Perl, A., Saad, S., Sahar, N., and D. Holland (1995) Establishment of long-term embryogenic cultures of seedless V. vinifera cultivars — a synergistic effect of auxins and the role of abscisic acid. Plant Sci. 104: 193–200.

    Article  CAS  Google Scholar 

  • Piven, N.M., Kuksova, V.B., Yuzephovich, L.V., and O.K. Makhorina (1991) Utilization of some cell technologies in grape breeding process. Biopolymers Cell 7: 66–72.

    Google Scholar 

  • Pramanik, S.K and J.E. Krochko (1992) Distribution of cytosolic messenger RNAs between polysomal and ribonucleoprotein complex fractions in alfalfa embryos-stage-specific translational repression of storage protein synthesis during early somatic embryo development. Plant Physiol. 99: 1590–1596.

    Article  PubMed  CAS  Google Scholar 

  • Rajasekaran, K. and M.G. Mullins (1979) Embryo and plantlets from cultured anthers of hybrid grapevines. J. Experim. Bot. 30: 399–407.

    Article  Google Scholar 

  • Rajasekaran, K. and M.G. Mullins (1983) The origin of embryos and plantlets from cultured anthers of hybrid grapevines. Am. J. Enol. Vitic. 34 (2): 108–113.

    CAS  Google Scholar 

  • Rajasekaran, K., Vine, J., and M.G. Mullins (1982) Dormancy in somatic embryos and seeds of Vitis: changes in endogenous abscisic acid during embryogeny and germination. Planta 154: 139–144.

    Article  CAS  Google Scholar 

  • Redenbaugh, K., Paasch, B.D., Nichol, J.W., Kossler, M.E., Viss, P.R., and K.A. Walker (1986) Somatic seeds: encapsulation of asexual plant embryos. Bio/Technol. 4: 797–801.

    Article  Google Scholar 

  • Redon, C., Albert, P., Lacorre-Arescaldino, I., Mauro, M.C., and J. Bierne (1996) Transition in histone 111 from globular to torpedo stage in grapevine somatic embryos. Proc. Intern. Congress French Soc. Developm. Biol. (SFBD), Maternal and zygotic genes during embryonic development, May 29–31, 1996, Caen, F, A57, p 35S.

    Google Scholar 

  • Redon, C., Albert, P., Lacorre-Arescaldino, I., Mauro, M.C., and J. Bierne (1999) Electrophoretic heterogeneity of grapevine histones. Am. J. Enol. Vitic. 50: 13–18.

    CAS  Google Scholar 

  • Reinert, J. (1958) Untersuchungen uber die Morphogenese an Gewebekulturen. Ber. Dtch. Ges. 71: 15.

    Google Scholar 

  • Reustle, G., Harst, M., and G. Alleweldt (1995) Plant regeneration of grapevine (Vitis sp.) protoplasts isolated from embryogenic tissues. Plant Cell Rep. 15: 238–241.

    Article  CAS  Google Scholar 

  • Robacker, C. (1993) Somatic embryogenesis and plant regeneration from muscadine grape leaf explants. Hort. Sci. 28: 53–55.

    Google Scholar 

  • Salunkhe, C.K., Rao, P.S., and M. Mhatre (1997) Induction of somatic embryogenesis and plantlets in tendrils of V. vinìfera L. Plant Cell Rep. 17: 65–7.

    Article  CAS  Google Scholar 

  • Salunkhe, C.K., Rao, P.S., and M. Mhatre (1999) Plantlet regeneration via somatic embryogenesis in anther callus of Vats latifolia L. Plant Cell Rep. 18: 670–673.

    Article  CAS  Google Scholar 

  • Schaefers, R.K., Pool, R.M., and D. Gonsalves (1994) Somatic embryogenesis from nucellar tissue for the elimination of viruses from grapevines. Proc. IXX ann. Meeting ASEV/Eastern Section, Cleveland, Ohio, Jul. 1994. Am. J. Enol. Vitic. 45: 472–473.

    Google Scholar 

  • Schneider, S., Reustle, G., and E. Zyprian (1996) Detection of somaclonal variation in grape-vine regenerants from protoplasts by RAPD-PCR. Vitis 35: 99–100.

    CAS  Google Scholar 

  • Scorza, R., Cordts, J.M., Ramming, D.W., and R.L. Emershad (1995) Transformation of grape (V. vinifera L.) Zygotic-derived somatic embryos and regeneration of transgenic plants. Plant Cell Rep. 14: 589–592.

    Article  CAS  Google Scholar 

  • Scorza, R., Cordts, J.M., Gray, D.J., Gonsalves, D., Emershad, R.L., and D.W. Ramming (1996) Producing transgenic Thompson Seedless grape (V vinìfera L.). J. Amer. Soc. Hort. Sci. 12: 616–619.

    Google Scholar 

  • Sefc, K.M., Ruckenbauer, P. and F. Regner (1997) Embryogenesis in microspore culture of Vitis subspecies. Vitis 36: 15–20.

    CAS  Google Scholar 

  • Stamp, J.A. and C.P. Meredith (1988a) Somatic embryogenesis from leaves and anthers of grapevine. Scientia Horne. 35: 235–250.

    Article  Google Scholar 

  • Stamp, J.A. and C.P. Meredith (1988b) Proliferative somatic embryogenesis from zygotic embryos of grapevine. J. Amer. Soc. Hort. Sci. 113: 941–945.

    Google Scholar 

  • Steward, F.C. (1958) Growth and development of cultivated cells. III. Interpretation of the growth from free cell to carrot plant. Am. J. Bot. 45: 709–715.

    Article  Google Scholar 

  • Steward, F.C., Mapes, M.O., and K. Mears (1958) Growth and organized development of cultures cells. Il. Organization in cultures grown from freely suspended cells. Am. J. Bot. 45: 705–708.

    Google Scholar 

  • Taken, K., Koshioka, M., Pharis, R.P., Rajasekaran, K., and M.G Mullins (1983) Endogenous gibberellin-like substances in somatic embryos of grape (V. vinìfera x Vitis rupestris) in relation to embryogenesis and the chilling requirement for subsequent development of mature embryos. Plant Physiol. 73: 803–808.

    Article  Google Scholar 

  • Taylor, R.L. (1967) The foliar embryos of Malaxispaludosa. Can. J. Bot. 45: 1553–1556.

    Article  Google Scholar 

  • Taylor, M.G. and I.K. Vasil (1996) Quantitative analysis of ultrastructural changes during zygotic and somatic embryogenesis in pearl millet (Pennisetum glaucum [L.] R. Br.). Sex Plant Reprod. 9: 286–298.

    Article  Google Scholar 

  • Toonen, M..A.J., Hendriks, T., Schmidt, E.D.L., Verhoven, H.A., van Kammen, A., and S. de Vries (1994) Description of somatic-embryo-forming single cells in carrot suspension cultures employing video cell tracking. Plants 194: 565–572.

    Article  CAS  Google Scholar 

  • Torregrosa, L. (1995) Biotechnologie de la vigne: les techniques de régénération in vitro (synthèse). Progr. Agric. Vitic. 112: 479–489.

    Google Scholar 

  • Torregrosa, L. (1998) A simple and efficient method to obtain stable embryogenic cultures from anthers of V. vinifera L. Vitis 37 (2): 91–92.

    Google Scholar 

  • Tsolova, V. and A. Atanassov (1996) Plant regeneration of Vitis sp. via repetitive embryogenesis. Biotechnol. & Biotechnol. Eq. 10/1996/ 1: 32–34.

    Google Scholar 

  • Vallania, R., Miaja, M.L., Vergano, G., Botta, R., Me, G., Zanetti, R., and I. Gribaudo (1994) Somatic embryogenesis in grapevine V vinifera L. In: Progress in Temperate Fruit Breeding, Schmidt H., Kellerhals M. (Eds), pp. 415–420.

    Google Scholar 

  • Vilaplana, M. and M.G. Mullins (1989) Regeneration of grapevine (Vitis spp.) in vitro: formation of adventitious buds on hypocotyls and cotyledons of somatic embryos. J. Plant Physiol. 134: 413–419.

    Article  CAS  Google Scholar 

  • Yarbrough, Y.A. (1932) Anatomical and developmental studies of the foliar embryos of Bryphyllum calycinum. Am. J. Bot. 19: 443–453.

    Article  Google Scholar 

  • Wolffe, A.P. (1996) Chromatin and gene regulation at the onset of embryonic development. Reprod. Nutr. Dev. 36: 581–606.

    PubMed  CAS  Google Scholar 

  • Zhu, Y.M., Hoshino, Y., Nakano, M., Takahashi, E., and M. Mii (1997) Highly efficient system of plant regeneration from protoplasts of grapevine (V. vinifera L.) through somatic embryogenesis by using embryo-genic callus culture and activated charcoal. Plant Sci. 123: 151–157.

    Article  CAS  Google Scholar 

  • Zou, C.J. and P.F. Li (1981) Induction of pollen plants of grape (V. vinifera L.). Acta Bot. Sinica 23: 79–81.

    Google Scholar 

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© 2001 Springer Science+Business Media Dordrecht

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Martinelli, L., Gribaudo, I. (2001). Somatic Embryogenesis in Grapevine. In: Roubelakis-Angelakis, K.A. (eds) Molecular Biology & Biotechnology of the Grapevine. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2308-4_13

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  • DOI: https://doi.org/10.1007/978-94-017-2308-4_13

  • Publisher Name: Springer, Dordrecht

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  • Online ISBN: 978-94-017-2308-4

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