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Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L.)

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Summary

Somatic embryo formation occurred from immature zygotic embryos within ovules of stenospermocarpic seedless grapes (Vitis vinifera L.), when cultured for two months on liquid Emershad/Ramming medium. Somatic embryos continued to proliferate after excision and transfer to Emershad/Ramming medium supplemented with 1 μM benzylaminopurine and 0.65% TC agar. Plant development from somatic embryos was influenced by genotype, medium, phase (liquid, agar), stage (torpedo, mature) and their interactions. Optimal plant development occurred on Woody Plant Medium supplemented with 1.5% sucrose + 1 μM benzylaminopurine + 0.3% activated charcoal and 0.65% TC agar.

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Abbreviations

ABA:

abscisic acid

BAP:

benzylaminopurine

ER:

Emershad/Ramming

GLM:

general linear model

MGLH:

multivariate general linear hypothesis

MS:

Murashige/Skoog

NaClO:

sodium hypochlorite

TC:

tissue culture

WP:

woody plant

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Authors and Affiliations

  1. USDA, ARS, HCRL, 2021 South Peach Avenue, 93727, Fresno, CA, USA

    R. L. Emershad & D. W. Ramming

Authors
  1. R. L. Emershad
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  2. D. W. Ramming
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Communicated by C. F. Quiros

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Emershad, R.L., Ramming, D.W. Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L.). Plant Cell Reports 14, 6–12 (1994). https://doi.org/10.1007/BF00233289

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  • DOI: https://doi.org/10.1007/BF00233289

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