Abstract
Genetic variability is an essential component of plant breeding and improvement programs. This variability is naturally generated in the living organisms via recombinational events and spontaneous mutations. A number of physical and chemical agents have been employed to enhance the extent of genetic variation. Genetic variability has also been detected in cultured plant cells. Tissue culture-associated variation (termed somaclonal variation) has been observed in morphological and biochemical characteristics, and in chromosome number and structure (14, 21, 38, 46, 55). Variation in chromosome numbers has been frequently observed in long-term plant cell cultures. This chromosome variation was subsequently shown to be recoverable in the regenerated plants, and seemingly offered a new source of genetic variation for plant improvement, particularly for vegetatively propagated crops, such as sugarcane (23, 28), and potato (42). Recent studies on tomato (19), rice (41, 52), and wheat (30) have revealed that variant characteristics expressed in the in vitro plants are transmitted to the progenies of sexually propagated crops. The recovery of single gene mutations in tomato (19) and wheat (30) have paved the way for exploitation of cell culture as a tool for introduction of genetic variation in seed propagated plants.
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References
AHLOOWALIA BS 1985 Limitations to the use of somaclonal variation in crop plants. In EEC Symposium on Somaclonal Variations and Crop Improvement, Gembloux, Belgium (in press)
AHUJA MR 1982 Isolation, culture, and fusion of protoplasts: problems and prospects. Silvae Genet 31: 66–77
AHUJA MR 1983 Somatic cell differentiation and rapid clonal propagation of aspen. Silvae Genet 32: 131–135
AHUJA MR 1984 Protoplast research in woody plants. Silvae Genet 33: 32–37
AHUJA MR, HJ MUHS 1985 In vitro techniques in clonal propagation of forest tree species. In A Schäfer-Menuhr, ed, In Vitro Techniques- Propagation and Long Term Storage, Martinus Nijhoff/Junk Publ, Dordrecht, pp 41–49
AUSTEN S, AC CASSELS 1983 Variation between plants regenerated from individual calli produced from separated potato stem callus cells. Plant Sci Lett 31: 107–114
BAYLISS MW 1980 Chromosome variation in plant tissues in culture. In IK Vasil, ed, Perspectives in Plant Cell and Tissue Culture. Int Rev Cytol Suppi 11A, Academic Press, New York, pp 113–144
BIONDI S, TA THORPE 1982 Clonal propagation of forest tree species. In AN Rao, ed, Tissue Culture of Economically Important Plants. COSTED and ANBS, Singapore, pp 197–204
BRELOH P, C KIRCH 1984 Waldsterben in der Bundesrepublik Deutschland. Allg For Z 39: 1265–1267
BRIGHT S, V JARRETT, R NELSON, G CRIESSEN, A KARP, J FRANKLIN, P NORBURY, J KUEH, S ROGNES, B MIFLIN 1983 Modification of agronomic traits using in vitro technology. In SH Mantell, H Smith, eds, Plant Biotechnology, Cambridge Univ Press, Cambridge, pp 251–265
BROWN CL, HE SOMMER 1982 Vegetative propagation of dicotyledonous trees. In JM Bonga, DJ Durzan, eds, Tissue Culture in Forestry, Martinus Nijhoff/Junk Publ, The Hague, pp 109–149
CHALEFF RS 1981 Genetics of Higher Plants, Cambridge Univ Press, Cambridge
CHATURVEDI HC, GC MITRA 1975 A shift in morphogenetic pattern in Citrus callus tissue during prolonged culture. Ann Bot 39: 683–687
D’AMATO F 1978 Chromosome number variation in cultured cells and regenerated plants. In TA Thorpe, ed, Frontiers of Plant tissue Culture 1978, Int Assoc Plant Tissue Cult, Univ Calgary, Alberta, pp 287–295
DAY A, THN ELLIS 1984 Chloroplast DNA deletions associated with wheat plants regenerated from pollen: possible basis for maternal inheritance of chloroplasts. Cell 39: 359–368
DINER AM, RL MOTT, HV AMERSON 1984 Cultured cells of white pine show genetic resistance to axenic blister rust hyphae. Science 224: 407–408
DULIEU H, M BARBIER 1982 High frequency of genetic variant plants regenerated from cotyledons of tobacco. In L Earle, Y Demarly, eds, Variability in Plants Regenerated from Tissue Culture, Praeger Press, New York, pp 211–119
DURZAN DJ 1984 Potential for genetic manipulation of forest trees: totipotency, somaclonal aberration, and trueness to type. In Proc Int Symp Recent Adv For Biotechnol, Traverse City, Michigan, pp 104–125
EVANS DA, WR SHARP 1983 Single gene mutations in tomato plants regenerated from tissue culture. Sience 221: 949–951
EVANS DA, WR SHARP, HP MEDINA-FILHO 1984 Somaclonal and gametoclonal variation. Am J Bot 71: 759–774
HEINZ GW, WP MEE 1969 Plant differentiation from callus tissues of Saccharum species. Crop Sci 9: 346–348
HEINZ GW, WP MEE 1971 Morphologic, cytogenetic, and enzymatic variation in Saccharum species hybrid clones derived from callus culture. Am J Bot 58: 257–262
HEINZ GW, M KRISHNAMURTHI, LG NICKELL, A MARETZKI 1977 Cell, tissue and organ culture in sugarcane improvement. In J Reinert, YPS Bajaj, eds, Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture, Springer Verlag, Berlin, pp 3–17
KARNOWSKY DF 1981 Potential of forest tree improvement via tissue culture. Bioscience 31: 114–120
KARP A, SWJ BRIGHT 1985 On the causes and origins of soma-clonal variation. In BJ Miflin, ed, Oxford Surveys of Plant Molecular & Cell Biology, vol 2, Oxford Univ Press, pp 199–234
KEMBLE RJ, JF SHEPARD 1984 Cytoplasmic DNA variation in potato protoclonal population. Theor Appl Genet 69: 211–216
KEMBLE RJ, Ris BRETTELL, RB FLAVELL 1982 Mitochondrial DNA analyses of fertile and sterile maize plants from tissue culture with the Texas male sterile cytoplasm. Theor Appl Genet 62: 213–217
KRISHNAMURTHI M 1982 Sugarcane improvement through tissue culture and review of progress. In AN Rao, ed, Tissue Culture of Economically Important Plants, COSTED and ANBS, Singapore, pp 70–77
LARKIN PJ, WR SCOWCROFT 1981 Somaclonal variation–a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214
LARKIN PJ, SA RYAN, RIS BRETTELL, WR SCOWCROFT 1984 Heritable somaclonal variation in wheat. Theor Appl Genet 67: 443–455
LEACH GN 1979 Growth in soil of plantlets produced by tissue culture, loblolly pine. Tappi 62: 59–61
LESTER DT, JG BERBEE 1977 Within clone variation among black poplar trees derived from callus cultures. For Sci 23: 122–131
LIU MC 1981 In vitro methods applied to sugarcane improvement. In TA Thorpe, ed, Plant Tissue Culture, Academic Press, New York, pp 299–323
LÖRZ H, WR SCOWCROFT 1983 Variability among plants and their progeny regenerated from protoplasts of Su/su hetero-zygotes of Nicotiana tabacum. Theor Appl Genet 66: 67–75
MALIGA P 1978 Resistance mutants and their use in genetic manipulation. In TA Thorpe, ed, Frontiers of Plant Tissue Culture 1978, Int Assoc Plant Tissue Cult, Univ Calgary, Alberta, pp 381–392
MCKEAND SE, HL ALLEN 1984 Nutritional and root development factors affecting growth of tissue culture plantlets of loblolly pine. Physiol Plant 61: 523–528
MURASHIGE T 1978 The impact of plant tissue culture on agriculture. In: TA Thorpe, ed, Frontiers of Plant Tissue Culture 1978, Int Assoc Plant Tissue Cult, Univ Calgary, Alberta, pp 15–26
MURASHIGE T, R NAKANO 1967 Chromosome complement as a determinant of morphogenetic potential of tobacco cells. Am J Bot 54: 963–970
ORTON TJ 1983 Experimental approaches to the study of soma-clonal variation. Plant Molec Biol Rep 1: 67–76
PATEL KR, GP BERLYN 1982 Genetic instability of multiple buds of Pinus coulteri regenerated from tissue culture. Can J For Res 12: 93–101
SCHAEFFER GW, FT SHARPE, PB CREGAN 1984 Variation for improved protein and yield from rice anther culture. Theor Appl Genet 67: 383–389
SHEPPARD JF 1981 Protoplasts as a source of disease resistance in plants. Annu Rev Phytopathol 19: 145–155
SHEPPARD JF, D BIDNEY, E SHAHIN 1980 Potato protoplasts in crop improvement. Science 208: 17–24
SINGH BD, BL HARVEY 1975 Does 2,4-D induce mitotic irregularities in plant tissue culture. Experientia 32: 785–787
SINGH BD, KN KAO, RA MILLER 1975 Karyotypic changes and selection pressure in Haplopappus gracilis suspension cultures. Can J Genet Cytol 17: 109–116
SKIRVIN RM 1978 Natural and induced variation in tissue culture. Euphytica 27: 241–266
SKIRVIN RM, J JANICK 1976 Tissue culture-induced variation in scented Pelargonium spp. J Am Soc Hort Sci 101: 281–290
SMITH SM, HE STREET 1974 The decline of embryogenic potential as callus and suspension cultures of carrot (Daucus carota L.) are serially subcultured. Ann Bot 38: 233–241
SPIEGEL-ROY P, J KOCHBA 1980 Embryogenesis in Citrus tissue cultures. Adv Biochem Engin 18: 27–48
SREE RAMULU K, P DIJKHUIS, S ROEST 1983 Phenotypic variation and ploidy level of plants regenerated from protoplasts of tetraploid potato (Solanum tuberosum L. cv. Bintje ). Theor Appl Genet 65: 329–338
SREE RAMULU K, P DIJKHUIS, S ROEST 1984 Genetic instability in protoclones of potato (Solanum tuberosum L. cv. Bintje): new types of variation after vegetative propagation. Theor Appl Genet 68: 515–519
SUN ZX, CZ ZHAO, KL ZHENG, XF QI, YP FU 1983 Somaclonal genetic of rice, Oryza sativa L. Theor Appl Genet 67: 67–73
SWEDLUND B, IK VASIL 1985 Cytogenetic characterization of embryogenic callus and regenerated plants of Pennisetum americanum (L.) K. Schum. Theor Appl Genet 69: 575–581
TABATA M, T OGINO, K YOSHIOKA, N YOSHIKAWA, N HIRAOKA 1979 Selection of cell lines with higher yield of secondary products. In TA Thorpe, ed, Frontiers of Plant Tissue Culture 1978, Int Assoc Plant Tissue Cult, Univ Calgary, Alberta, pp 213–222
THOMAS E, PJ KING, I POTRYKUS 1979 Improvement of crop plants via single cells in vitro–an assessment. Z Pflanzenzucht 82: 1–30
THOMAS E, SWJ BRIGHT, J FRANKLIN, VA LANCASTER, BJ MIFLIN, R GIBSON 1982 Variation amongst protoplast derived potato plants (Solanum tuberosum cv.“Maris Bard”). Theor Appl Genet 62: 65–68
VASIL IK, V VASIL 1980 Clonal propagation. In IK Vasil, ed, Perspectives in Plant Cell and Tissue Culture, Int Rev Cytol Suppl 11A, Academic press, New York, pp 145–173
VASIL IK, MR AHUJA, V VASIL 1979 Plant tissue cultures in genetics and plant breeding. Adv Genet 20: 127–215
WIDHOLM JM 1977 Selection and characterization of aminoacid analog resistant plant cell cultures. Crop Sci 17: 597–600
WOOI KG, CY WONG, RH VANER, C BAKASAWIT 1982 Genetic stability of oil plam callus cultures. In A Fujiwara, ed, Plant Tissue Culture 1982, Proc 5th Int Cong Plant Tissue Cult, Tokyo, pp 749–750
YURKOVA GN, BA LEVENKO, PV NOVOZHILOV 1982 Plant regeneration in wheat tissue culture. Biochem Physiol Pflanz 177: 337–344
ZENG JZ 1983 Application of anther culture techniques to crop improvement in China. In SK Sen, KL Giles, eds, Plant Cell Culture in Crop Improvement, Plenum Press, New York, pp 351–363
ZENK MH 1978 The impact of plant cell culture on industry. In TA Thorpe, ed, Frontiers of Plant Tissue Culture, Int Assoc Plant Tissue Cult, Univ Calgary, Alberta, pp 1–13
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Ahuja, M.R. (1987). Somaclonal Variation. In: Bonga, J.M., Durzan, D.J. (eds) Cell and Tissue Culture in Forestry. Forestry Sciences, vol 24-26. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0994-1_16
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DOI: https://doi.org/10.1007/978-94-017-0994-1_16
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