Skip to main content
SpringerLink
Log in

Resistance to Root-Knot Nematodes in Tomato

Towards the molecular cloning of the Mi-1 locus

  • Chapter
Cellular and Molecular Aspects of Plant-Nematode Interactions

Part of the book series: Developments in Plant Pathology ((DIPP,volume 10))

Abstract

Host resistance to three major species of root-knot nematodes, Meloidogyne incognita, M. javanica and M. arenaria, has been introgressed in the cultivated tomato (Lycopersicon esculentum Mill.) from its wild relative L. peruvianum. The trait is dominant and has been located at a single locus, Mi-1, on chromosome 6. The Mi-1-conferred resistance is associated with a hypersensitive response in nematode-infected tissues; it is not efficient at soil temperature higher than 28 °C, and can be overcome by some “virulent” pathotypes as well as by the species M. hapla. Novel resistances, that have broader specificity to root-knot nematodes and are heat-stable, are pursued and identified in the wild tomato germplasm.

Molecular cloning of the Mi-1 locus, based on its map position, has been undertaken and reaches its final step. Some major obstacles due to the proximity of the Mi-1 locus to the centromere had to be overcome following the positional cloning approach, namely the presence of repetitive sequences and the suppression of recombination around Mi-1. Narrowing down the Mi-1 locus to less than 50 kb made complementation analysis possible. Transformation of susceptible tomato genotype with genomic and cDNA clones from the 50 kb region will reveal the sequence of Mi-1.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 16.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. Aarts, J.M.M.J.G., Hontelez, J.G.J., Fischer, P., Verkerk, R., van Kammen, A., and Zabel, P. (1991) Acid phosphatase-11, a tightly linked molecular marker for root-knot nematode resistance in tomato: from protein to gene, using PCR and degenerate primers containing deoxyinosine, Plant Mol. Biol. 16, 647–661.

    Article  CAS  PubMed  Google Scholar 

  2. Ammati, M., Thomason, I.J., and McKinney, H.E. (1986) Retention of resistance to Meloidogyne incognita in Lycopersion genotypes at high soil temperature, J. Nematol. 18, 491–495.

    CAS  PubMed Central  PubMed  Google Scholar 

  3. Ammati, M., Thomason, I.J., and Roberts, P.A. (1985) Screening Lycopersion spp. for new genes imparting resistance to root-knot nematodes (Meloidogyne spp.), Plant Dis. 69, 112–115.

    Google Scholar 

  4. Bailey, D.M. (1941) The seedling test method for root-knot nematode resistance, Proc. Am. Soc. Hortic. Sci. 38, 573–575.

    Google Scholar 

  5. Barham, W.S., and Winstead, N.N. (1957) Inheritance of resistance to root-knot nematodes, Tomato Genet. Coop. Rep. 7, 3.

    Google Scholar 

  6. Briggs, S.P., and Johal, G.S. (1994) Genetic pattern of plant host-parasite interactions, Trends Genet. 10, 12–16.

    Article  CAS  PubMed  Google Scholar 

  7. Cap, G.B., Roberts, P.A., Thomason, I.J., and Murashige, T. (1991) Embryo culture of Lycopersicon esculentum x L. peruvianum hybrid genotypes possessing heat-stable resistance to Meloidogyne incognita, J.Amer Soc.Hort.Sci. 116, 1082–1088.

    Google Scholar 

  8. Cap, G.B., Roberts, P.A. and Thomason, I.J. (1993) Inheritance of heat-stable resistance to Meloidogyne incognita in L. peruvianum and its relationship to the Mi gene, Theor. Appl.. Genet 85, 777–783.

    CAS  PubMed  Google Scholar 

  9. Castagnone-Sereno, P., Bongiovanni, M., Palloix, A., and Dalmasso, A. (1996) Selection for Meloidogyne incognita virulence against resistance genes from tomato and pepper and specificity of the virulence/resistance determinants, European J. Plant Pathology, in press.

    Google Scholar 

  10. Cook, R. (1991) Resistance in plants to cyst and root-knot nematodes, Agricult. Zool. Rev. 4, 213–240.

    Google Scholar 

  11. Dean, J.L., and Struble, F.B. (1953) Resistance and susceptibility to root-knot nematodes in tomato and sweet potato, Phytopathology 43, 290.

    Google Scholar 

  12. Dropkin, V.H. (1969) The necrotic reaction of tomatoes and other hosts resistant to Meloidogyne: reversal by temperature, Phytopathology 59, 1632–1637.

    Google Scholar 

  13. Fassuliotis, G. (1987) Genetic basis of plant resistance to nematodes, in Veech, J.A. and Dickson, D.W.(eds.), Vistas on nematology:, Soc. of Nematologists, Inc. Hyattsville, Maryland, pp.364–371.

    Google Scholar 

  14. Flor, H.H. (1956) The complementary genetic systems in flax and flax rust, Advan. Genet. 8, 29–54.

    Article  Google Scholar 

  15. Frazier, W.A., and Dennett, R.K. (1949) Isolation of Lycopersicon esclentum type tomato lines essentially homozygous resistant to root-knot, Proc. Am. Soc. Hortic. Sci. 5, 225–236.

    Google Scholar 

  16. Ganal, M.W, and Tanksley, S.D. (1996) Recombination around the Tm2a and Mi resistance genes in different crosses of Lycopersicon peruvianum, Theor. Appl. Genet. 92, 101–108.

    Article  CAS  PubMed  Google Scholar 

  17. Gillbert, J.C. (1958) Some linkage studies with the Mi gene for resistance to root-knot, Tomato Genet. Coop. Rep. 8, 15–17.

    Google Scholar 

  18. Gillbert, J.C., and McGuire, D.C. (1956) Inheritance of resistance to several root-knot from Meloidogyne incognita in commercial type tomatos, Proc. Am. Soc. Hortic. Sci 68, 437–442.

    Google Scholar 

  19. Ho, J.Y., Weide, R., van Wordragen, M., Lambert, K., Koornneef, M., Zabel, P., Williamson, V.M. (1992) The root-knot nematode resistance gene (Mi) in tomato: construction of a molecular linkage map and identification of dominant cDNA markers in resistant genotypes, Plant J. 2, 971–982.

    CAS  PubMed  Google Scholar 

  20. Holzman, O.V. (1965) Effect of soil temperature on resistance of tomato to root-knot nematode (Meloidogyne incognita), Phytopathology 55, 990–992.

    Google Scholar 

  21. Janssen, R., Bakker, J., and Gommers, F. (1991) Mendelian proof for a gene-for-gene relationship between virulence of Globodera rostochiensis and the H1 resistance gene in Solanum tuberosum spp. andigena CPC 1673, Revue Nematol. 14, 213–219.

    Google Scholar 

  22. Klein-Lankhorst, R,. Rietveld, P., Machiels, B., Verkerk, R., Weide, R., Gebhardt, C., Koornneef, M., and Zabel, P. (1991) RFLP markers linked to the root-knot nematode resistance gene Mi in tomato, Theor. Appl. Genet. 81, 661–667.

    Article  CAS  PubMed  Google Scholar 

  23. Liharska, T.B., Koornneef, M., van Wordragen, M., van Kammen, A., and Zabel, P. (1996) Tomato chromosome 6: effect of alien chromosomal segments on recombinant frequencies, Genome 39, in press.

    Google Scholar 

  24. Lobo, M., Navarro, R., and Munera, G. (1988) Meloidogyne incognita and Meloidogyne javanica resistance in Lycopersicon species, Tomato Genet. Coop. Rep. 38, 31–32.

    Google Scholar 

  25. Martin, G.B., Ganal, M.W., and Tanksley, S.D. (1992) Construction of yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci, Mol. Gen. Genet. 233, 25–32.

    Article  CAS  PubMed  Google Scholar 

  26. Medina-Filho, H., and Tanksley, S.D. (1983) Breeding for nematode resistance. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (Eds) Handbook of plant cell culture, Vol 1, Techniques for propagation and breeding, Mac Millan, New York, pp. 904–923.

    Google Scholar 

  27. Messenguer, R., Ganal, M., de Vicente, M.C., Young, N.D., Bolkan, H., and Tanksley, S.D. (1991) High resolution RFLP map around the root-knot nematode resistance gene (Mi) in tomato, Theor. Appl. Genet. 82, 529–536.

    Google Scholar 

  28. Paulson, R.E., and Webster, J.M. (1972) Ultrastructure of the hypersensitive reaction in roots of tomato, Lycopersicon esculentum L., to infection by root-knot nematode, Meloidogyne incognita, Physiol. Plant Pathol. 2, 227–234.

    Article  Google Scholar 

  29. Rick, CM., and Fobes, J.F. (1974) Association of an allozyme with nematode resistance, Tomato Genet. Coop. Rep. 24: 25.

    Google Scholar 

  30. Roberts, P.A., Dalmasso, A., Cap, G.B., and Castagnone-Sereno, P. (1990) Resistance in Lycopersicon peruvianum to isolates of Mi gene-compatible Meloidogyne populations, J.Nematol. 22, 585–589.

    CAS  PubMed Central  PubMed  Google Scholar 

  31. Romshe, F.A. (1942) Nematode resistance test of tomato, Proc. Am. Soc. Hortic. Sci. 40, 423.

    Google Scholar 

  32. Sasser, J.N., Hartman, K.M., Carter, C.E. (1987) Summary of preliminary crop germplasm evaluation for resistance to root-knot nematodes, NC State University/US Agency Int. Dev. Raleigh NC, pp. 88.

    Google Scholar 

  33. Scott, J.W., Emmons, C.L., Overman, A.J., and Somodi, G.C. (1991) Introgression and genetics of heat stable nematode resistance from Lycopersicon peruvianum, Tomato Genet. Coop. Rep. 41, 46.

    Google Scholar 

  34. Sidhu, G.S., and Webster, J.M. (1981) The genetics of plant-nematode parasitic systems, Bot. Rev. 47, 387–419.

    Article  Google Scholar 

  35. Smith, P.G. (1944) Embryo culture of a tomato species hybrid, Proc. Am.Soc.Hortic. Sci. 44, 413–416.

    Google Scholar 

  36. Staskawicz, B.J., Ausubel, F.A., Baker, B.J., Ellis, J.G., and Jones, J.D.G. (1995) Molecular genetics of plant disease resistance, Science 268, 661–667.

    Article  CAS  PubMed  Google Scholar 

  37. Tanksley, S.D., Ganal, M.W., and Martin, G.B. (1995) Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes, Trends Genet. 11, 63–68.

    Article  CAS  PubMed  Google Scholar 

  38. Tanksley, S.D., Ganal, M.W., Prince, J.P., de Vincente, M.C., Bonierbale, M.W., Broun, P., Fulton, T.M., Giovanonni, J.J., Grandillo, S., Martin, G.B., Messeguer, R., Miller, J.C., Miller, L., Paterson, A.H., Pineda, O., Roder, M.S., Wing, R.A., Wu, W., Young, N.D. (1992) High density molecular linkage maps of the tomato and potato genomes, Genetics 132, 1141–1160.

    CAS  PubMed Central  PubMed  Google Scholar 

  39. Van Daelen, R. (1995) Towards isolation of the tomato root-knot nematode resistance gene Mi via positional cloning, PhD thesis, Wageningen Agricultural University.

    Google Scholar 

  40. Van Daelen, R.A.J.J., Grebens, F., van Ruissen, F., Aarts, J., Hontelez, J., and Zabel, P. (1993) Long-range physical maps of two loci (Aps-1 and GP79) flanking the root-knot nematode resistance gene (Mi) near the of tomato chromosome 6, Plant. Mol. Biol. 23, 185–192.

    Article  PubMed  Google Scholar 

  41. Van Wordragen, M.F., Weide, R., Liharska, T.B., van der Steen, A., Koornneef, M., and Zabel, P. (1994) Genetic and molecular organisation of the short arm and pericentromeric region of tomato chromosome 6, Euphytica 79, 169–174.

    Article  Google Scholar 

  42. Veremis, J.C., and Roberts, P.A. (1996a) Differentiation of Meloidogyne incognita and M. arenaria novel resistance phenotypes in Lycopersiconperuvianum and derived bridge lines, Theor. Appl. Genet., in press.

    Google Scholar 

  43. Veremis, J.C., and Roberts, P.A.(1996b) Relationships between Meloidogyne incognita resistance genes in Lycopersicon peruvianum differentiated by heat sensitivity and nematode virulence, Theor. Appl. Genet., in press.

    Google Scholar 

  44. Veremis, J.C., and Roberts, P.A.(1996c) Identification of resistance to Meloidogyne javanica in the Lycopersicon peruvianum complex, Theor. Appl. Genet., in press.

    Google Scholar 

  45. Weide, R., van Wordragen, M., Klein-Lankhorst, R., Verkerk, R., Hanhart, C., Liharska, T., Pap, E., Stam, P., Zabel, P., and Koornneef, M. (1993) Integration of the classical and molecular linkage maps of tomato chromosome 6, Genetics 135, 1175–1186.

    CAS  PubMed Central  PubMed  Google Scholar 

  46. Williamson V.M., and Colwell, G. (1991) Acid phosphatase-1 from nematode resistant tomato: isolation and characterization of the gene, Plant Physiol. 97, 139–146.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  47. Williamson V.M., Ho, J.Y., Wu, F.F., Miller, N., and Kaloshian, I. (1994) A PCR-based marker tightly linked to the nematode resistance gene, Mi, in tomato, Theor. Appl. Genet. 87, 757–763.

    Article  CAS  PubMed  Google Scholar 

  48. Yaghoobi, J., Kaloshian, I., Wen, Y., and Williamson, V. M. (1995) Mapping a new nematode resistance locus in Lycopersicon peruvianum, Theor. Appl. Genet. 91, 457–446

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology, Wageningen Agricultural University, 6703 HA, The Netherlands

    Tsvetana B. Liharska

  2. Department of Nematology, University of California, Davis, CA, 95616, USA

    Valerie M. Williamson

Authors
  1. Tsvetana B. Liharska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Valerie M. Williamson
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Biologia, University of Autonoma de Madrid, Madrid, Spain

    C. Fenoll

  2. Institut für Phytopathologie, Universität Kiel, Kiel, Germany

    F. M. W. Grundler

  3. MOGEN International nv, Leiden, The Netherlands

    S. A. Ohl

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Liharska, T.B., Williamson, V.M. (1997). Resistance to Root-Knot Nematodes in Tomato. In: Fenoll, C., Grundler, F.M.W., Ohl, S.A. (eds) Cellular and Molecular Aspects of Plant-Nematode Interactions. Developments in Plant Pathology, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5596-0_15

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-5596-0_15

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6360-9

  • Online ISBN: 978-94-011-5596-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation