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Molecular Farming in Plants: An Overview

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Molecular Farming in Plants: Recent Advances and Future Prospects

Abstract

Protein-based biopharmaceuticals have become increasingly important due to a combination of their bioreactivity, specificity, safety and overall success rate. Escherichia coli, yeast and animal cells have traditionally been used as heterologous expression systems for production of pharmaceutical proteins. However, these conventional expression systems are often limited by high production costs, potential risks of product contamination, and the complexity and difficulty of scale-up to industrial production. Plants have emerged as a promising alternative expression system for production of pharmaceutical proteins because they offer several potential advantages, including low production costs, ease of scale-up to commercial quantities of production and reduced risk of product contamination by mammalian viruses or toxins. Plants are already being used to produce antibodies, vaccines, growth factors and many other proteins of pharmaceutical importance. The use of plants as factories for production of recombinant pharmaceutical proteins, including industrial enzymes, is now more commonly referred to as molecular farming. In this chapter, we discuss the technological basis of molecular farming in plants, with a focus on host systems and approaches/strategies developed to maximize protein yields and to ensure efficient recovery and purification of plant-made recombinant products.

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Authors and Affiliations

  1. Transplantation Immunology Group, Lawson Health Research Institute, London, ON, N6A 4G5, Canada

    Shengwu Ma

  2. Department of Biology, University of Western Ontario, London, ON, N6A 5B7, Canada

    Shengwu Ma

  3. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford St., London, ON, N5V 4T3, Canada

    Aiming Wang

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  1. Agriculture and Agri-Food Canada, Sandford Street 1391, London, N5V 4T3, Ontario, Canada

    Aiming Wang

  2. , Immunology and Transplantation, Lawson Health Research Institute/Plantig, South Street 375, London, N6A4G5, Ontario, Canada

    Shengwu Ma

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Ma, S., Wang, A. (2012). Molecular Farming in Plants: An Overview. In: Wang, A., Ma, S. (eds) Molecular Farming in Plants: Recent Advances and Future Prospects. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2217-0_1

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