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Date Palm Cell and Protoplast Culture

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Date Palm Biotechnology

Abstract

This chapter describes the current status of cell and protoplast cultures in date palm (Phoenix dactylifera L.). Critically important steps toward plant regeneration from recalcitrant date palm protoplasts have been achieved in the recent past. Callus regeneration was achieved in commercial cvs. Deglet Noor, Takerboucht, Barhee and Zaghloul. The use of feeder layer was the main factor for inducing cell divisions as well as subsequent microcallus and callus formation. Presently, a protoplast-to-plant system has been reported for more than 400 species, of which the family Solanaceae is predominantly represented. In crops like potato, tobacco, brassica, citrus and eggplant seed companies are using protoplast fusion based technology to produce new commercial varieties. In the last decade however, little progress has been made regarding protoplast regeneration from the so-called recalcitrant species which includes date palm. There are certain similarities between some protoplast systems and mammalian stem cells. More collaboration between animal and plant scientists is highly encouraged, which would be useful for both parties to help address these challenges.

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Authors and Affiliations

  1. Department of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, Canada

    A. Assani

  2. Laboratoire de Recherche sur les Zones Arides (LRZA), Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP 31 El Alia Bab Ezzouar, Algiers, Algeria

    D. Chabane & N. Bouguedoura

  3. Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada

    H. Shittu

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  1. A. Assani
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  2. D. Chabane
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  3. H. Shittu
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  4. N. Bouguedoura
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Correspondence to A. Assani .

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Editors and Affiliations

  1. , Department of Applied Biology, University of Helsinki, Latokartanonkaari 5, Helsinki, 00014, Finland

    Shri Mohan Jain

  2. , Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, 31982, Saudi Arabia

    Jameel M. Al-Khayri

  3. Middlebrook Ave 3726, Cincinnati, 45208, Ohio, USA

    Dennis V. Johnson

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Assani, A., Chabane, D., Shittu, H., Bouguedoura, N. (2011). Date Palm Cell and Protoplast Culture. In: Jain, S., Al-Khayri, J., Johnson, D. (eds) Date Palm Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1318-5_29

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