Abstract
Earlier, polyploidy was detected on the basis of only a few morphological, cytological and genetic criteria. However, it may not be correct to characterize polyploidy on the above structural basis only because the chromosome pairing behaviour is also affected by other factors (Otto 2007). Polyploids have distinct morphology as compared to their diploid counterparts. Increased cell size that results in thicker and broader leaves and larger flowers and fruits is often associated with polyploidy. These characters are often used to identify putative polyploids, when screening of large number of plants has to be done. Larger pollen size and stomata are also often used to detect polyploidy. Although Stebbins (1950) had successfully used morphological criteria as evidence for finding the progenitor of the B-genome, yet some controversy still exists. Besides morphology, biochemical compounds, such as secondary metabolites, seed proteins and isozymes, have been also used to investigate progenitor of allopolyploids.
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References
Barcaccia G, Meneghetti S, Albertini E, Triest L, Lucchin M (2003) Linkage mapping intetraploid willows: segregation of molecular markers and estimation of linkage phases support an allotetraploid structure for Salix alba × Salix fragilis interspecific hybrids. Heredity 90:169–180
Barker MS, Kane NC, Matvienko M, Kozik A, Michelmore RW, Knapp SJ, Rieseberg LH (2008) Multiple paleopolyploidizations during the evolution of the Compositae reveal parallel patterns of duplicate gene retention after millions of years. Mol Biol Evol 25:2445–2455
Barker MS, Vogel H, Schranz ME (2009) Paleopolyploidy in the Brassicales: analyses of the Cleome transcriptome elucidate the history of genome duplications in Arabidopsis and other Brassicales. Genome Biol Evol 1:391–399
Bennett MD, Leitch IJ (2005) Nuclear DNA amounts in angiosperms: progress, problems and prospects. Ann Bot 95(1):45–90
Bennett ST, Kenton AY, Bennett MD (1992) Genomic in situ hybridization reveals the allopolyploid nature of Milium montianum (Gramineae). Chromosoma 101:420–424
Besnard G, Garcia-verdugo C, Rubiodecasas R, Treier UA, Galland N, Vargas P (2008) Polyploidy in the olive complex (Olea europaea): evidence from flow cytometry and nuclear microsatellite analyses. Annal Bot 101:25–30
Blanc G, Wolfe KH (2004) Functional divergence of duplicated genes formed by polyploidy during Arabidopsis evolution. Plant Cell 16:1679–1691
Blanc G, Barakat A, Guyot R, Cooke R, Delseny M (2000) Extensive duplication and reshuffling in the Arabidopsis genome. Plant Cell 12:1093–1101
Bowers JE, Chapman BA, Rong J, Paterson AH (2003) Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events. Nature 422:433–438
Chester M, Leitch AR, Soltis PS, Soltis DE (2010) Review of the application of modern cytogenetic methods (FISH/GISH) to the study of reticulation (polyploidy/hybridisation). Genes 1:166–192
Cifuentes M, Eber F, Lucas MO, Lode M, Chèvre AM, Jenczewski E (2010) Repeated polyploidy drove different levels of crossover suppression between homoeologous chromosomes in Brassica napus allohaploids. Plant Cell 22:2265–2276
Cui LY, Wall PK, Leebens-Mack JH, Lindsay BG, Soltis DE, Doyle JJ, Soltis PS, Carlson JE, Arumuganathan K, Barakat A, Albert VA, Ma H, DePamphills CW (2006) Widespread genome duplications throughout the history of flowering plants. Genome Res 16:738–749
Dar TH, Raina SN, Goel S (2013) Molecular analysis of genomic changes in synthetic autotetraploids Phlox drummondii Hook. Biol J Linn Soc 110:591–605
Esselink GD, Nybom H, Vosman B (2004) Assignment of allelic configuration in polyploids using the MAC-PR (microsatellite DNA allele counting—peak ratios) method. Theor Appl Genet 109:402–408
Gaeta RT, Pires JC, Iniguez-Luy F, Leon E, Osborn TC (2007) Genomic changes in resynthesized Brassica napus and their effect on gene expression and phenotype. Plant Cell 19:3403–3417
Gupta PK (2007) Cytogenetics. Rastogi Publications, Meerut
Hasterok R, Draper J, Jenkins G (2004) Laying the cytotaxonomic foundations of a new model grass, Brachypodium distachyon L. Chromosome Res 12:397–403
Jaillon O, Aury JM, Noel B, Policriti A, Clepet C et al (2007) French-Italian public consort. Grapevine genome charact. The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature 449:463–467
Julier B, Flajoulot S, Barre P, Cardinet G, Santoni S, Huguet T, Huyghe C (2003) Construction of two genetic linkage maps in cultivated tetraploid alfalfa (Medicago sativa) using microsatellite and AFLP markers. BMC Plant Biol. https://doi.org/10.1186/1471-2229-3-9
Kenton A, Parokonny AS, Gleba YY, Bennett MD (1993) Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics. Mol Gen Genet 240:159–169
Kron P, Suda J, Husband BC (2007) Applications of flow cytometry to evolutionary and population biology. Ann Rev Eco Evol Syst 38:847–876
Leitch IL, Bennett MD (1997) Polyploidy in angiosperms. Trends Plant Sci 2:470–476
Leuchtenberger C (1958) Quantitative determination of DNA in cells by Feulgen microspectophotometry. In: Danielli JF (ed) General cytochemical methods. Academic Press, New York, pp 219–278
Li J, Das K, Liu J, Fu G, Li Y, Tobias C, Wu R (2012) Statistical models for genetic mapping in polyploids: challenges and opportunities. Methods Mol Biol 871:245–261
Liu B, Vega JM, Feldman M (1998) Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops. II. Changes in low-copy coding DNA sequences. Genome 41:535–542
Ma JF, Shen R, Zhao Z, Wissuwa M, Takeuchi Y, Ebitani T, Yano M (2002) Response of rice to Al stress and identification of quantitative trait loci for Al tolerance. Plant Cell Physiol 43:652–659
Madlung A, Tyagi AP, Watson B, Jiang H, Kagochi T, Doerge RW, Martienssen R, Comai L (2005) Genomic changes in synthetic Arabidopsis polyploids. Plant J 41:221–230
McLeish J, Sunderland N (1961) Measurements of deoxyribonucleic acid (DNA) in higher plants by Feulgen photometry and chemical methods. Exp Cell Res 24:527–540
Nicolas SD, Leflon M, Monod H, Eber F, Coriton O, Huteau V, Chèvre AM, Jenczewski E (2009) Genetic regulation of meiotic cross-overs between related genomes in Brassica napus haploids and hybrids. Plant Cell 21:373–385
Oliver R, Jellen E, Ladizinsky G, Korol A, Kilian A, Beard J, Dumlupinar Z, Wisniewski-Morehead N, Svedin E, Coon M et al (2011) New Diversity Arrays Technology (DArT) markers for tetraploid oat (Avena magna Murphy et Terrell) provide the first complete oat linkage map and markers linked to domestication genes from hexaploid A. sativa L. Theor Appl Genet 123:1159–1171
Otto SP (2007) The evolutionary consequences of polyploidy. Cell 131:452–462
Paterson AH, Bowers JE, Chapman BA (2004) Ancient polyploidization predating divergence of the cereals, and its consequences for comparative genomics. Proc Nat Acad Sci U S A 101:9903–9908
Pires JC, Lim KY, Kovarik A, Matyasek R, Boyd A, Leitch AR, Leitch IJ, Bennett MD, Soltis PS, Soltis DE (2004) Molecular cytogenetic analysis of the recently evolved Tragopogon (Asteraceae) allopolyploids reveals a karyotype that is additive of the diploid progenitors. Am J Bot 91:1022–1035
Porceddu A, Albertini E, Barcaccia G, Falistocco E, Falcinelli M (2002) Linkage mapping in apomictic and sexual Kentucky bluegrass (Poa pratensis L.) genotypes using a two way pseudo-testcross strategy based on AFLP and SAMPl markers. Theor Appl Genet 104:273–280
Raina SN, Raini V (2001) GISH technology in plant genome research. Methods Cell Sci 23:83–104
Reinisch AJ, Dong JM, Brubaker CL, Stelly DM, Wendel JF, Paterson AH (1994) A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium bar badense: chromosome organization and evolution in a disomic polyploid genome. Genetics 138(3):829–847
Salmon A, Ainouche ML, Wendel JF (2005) Genetic and epigenetic consequences of recent hybridization and polyploidy in Spartina (Poaceae). Mol Ecol 14:1163–1175
Sampedro J, Yi L, Robert EC, de Pamphilis C, Cosgrove DJ (2005) Use of genomic history to improve phylogeny and understanding of births and deaths in a gene family. Plant J 44:409–419
Schlueter JA, Dixon P, Granger C, Grant D, Clark L, Doyle JJ, Shoemaker RC (2004) Mining EST databases to resolve evolutionary events in major crop species. Genome 47:868–876
Shi T, Huang H, Barker MS (2010) Ancient genome duplications during the evolution of kiwifruit (Actinidia) and related Ericales. Ann Bot 106:497–504
Shoemaker RC, PolzinK LJ, SpechtJ BEC, OlsonT YN, ConcibidoV WJ, Tamulonis JP, Kochert G, Boerma HR (1996) Genome duplication in soybean (Glycine subgenus soja). Genetics 144:329–338
Song K, Lu P, Tang K, Osborn TC (1995) Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution. Proc Natl Acad Sci USA 92:7719–7723
Stace CA (2000) Cytology and cytogenetics as a fundamental taxonomic resource for the 20th and 21st centuries. Taxon 49:451–477
Stebbins GL (1950) Variation and evolution in plants. Columbia University Press, New York
Sterck L, Rombauts S, Jansson S, Sterky F, Rouzé P, Van de Peer Y (2005) EST data suggest that poplar is an ancient polyploid. New Phytol 167(1):165–170
Szadkowski E, Eber F, Huteau V, Lode M, Huneau C, Belcram H, Coriton O, Manzanares-Dauleux MJ, Delourme R, King GJ et al (2010) The first meiosis of resynthesized Brassica napus, a genome blender. New Phytol 186:102–112
Udall JA, Wendel JF (2006) Polyploidy and crop improvement. Crop Sci 46:3–14
Van Dijk T, Noordijk Y, Dubos T, Bink M, Meulenbroek B, Visser R, van de Weg E (2012) Microsatellite allele dose and configuration establishment (MADCE): an integrated approach for genetic studies in allopolyploids. BMC Plant Biol 12. https://doi.org/10.1186/1471-2229-12-25
Vision TJ, Brown DG, Tanksley SD (2000) The origins of genomic duplications in Arabidopsis. Science 290:2114–2117
Wu KK, Burnquist W, Sorrells ME, Tew TL, Moore PH, Tanksley SD (1992) The detection and estimation of linkage in polyploids using single-dose restriction fragments. Theor Appl Genet 83:294–300
Wu R, Ma CX, Casella G (2004) A bivalent polyploid model for mapping quantitative trait loci in outcrossing tetraploids. Genetics 166:581–595
Yu J, Wang J, Lin W, Li S, Li H et al (2005) The genomes of Oryza sativa: a history of duplications. PLoS Biol 3:e38
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Dar, TUH., Rehman, RU. (2017). Detection of Polyploidy. In: Polyploidy: Recent Trends and Future Perspectives. Springer, New Delhi. https://doi.org/10.1007/978-81-322-3772-3_4
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DOI: https://doi.org/10.1007/978-81-322-3772-3_4
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