Abstract
Citrus and Poncirus belong to the family Rutaceae. Citrus includes many commercially important fruit species such as sweet orange (C. sinensis), mandarin (C. reticulata), lemon (C. limon), grapefruit (C. paradisi), and is grown in over 100 countries in tropical and subtropical areas spreading over approximately 40° latitude. On the other hand, Poncirus includes only one species of trifoliate orange. (P. trifoliata), which is a deciduous shrub widely grown in Japan and China. It is an important rootstock which possesses cold hardiness, and is resistant to phytophthora, nematode, and tristeza virus (Saunt 1990). The morphology of Citrus and Poncirus is very different; however, their genomes are similar and the chromosome number is 2n = 18. These two genera are sexually compatible and the most widely grown sexual hybrid is Troyer citrange, which has become a very important rootstock in many countries.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Aviv D, Arzee-Gonen P, Bleichman S, Galun E (1984) Novel alloplasmic Nicotiana plants by “donor-recipient” protoplast fusion: cybrids having N. tabacum or N. sylvestris nuclear genomes and either or both plastomes and chondriomes from alien species. Mol Gen Genet 196: 244–253
Burger DW, Hackett WP (1982) The isolation, culture and division of protoplasts from Citrus cotyledons. Physiol Plant 56: 324–328
Button J, Kochba J (1977) Tissue culture in the Citrus industry. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 70–92
Grosser JW, Chandler JL (1987) Aseptic isolation of leaf protoplast from Citrus, Poncirus, Citrus × Poncirus hybrids and Severinia for use in somatic hybridization experiments. Sci Hortic 31: 253–257
Grosser JW, Gmitter FG Jr, Chandler JL (1988a) Intergeneric somatic hybrid plants of Citrus sinensis cv. Hamlin and Poncirus trifoliata cv. Flying Dragon. Plant Cell Rep 7: 5–8
Grosser JW, Gmitter FG Jr, Chandler JL (1988b) Intergeneric somatic hybrid plants from sexually incompatible woody species: Citrus sinensis and Severinia disticha Theor Appl Genet 75: 397–401
Grosser JW, Moore GA, Gmitter FG Jr (1989) Interspecific somatic hybrid plants from the fusion of Key lime (Citrus aurantifolia) with Valencia sweet orange (Citrus sinensis) protoplasts. Sci Hortic 39: 23–29
Grosser JW, Frederick G, Gmitter FG Jr (1990a) Somatic hybridization of Citrus with wild relatives for germplasm enhancement and cultivar development. HortScience 25(2): 147–151
Grosser JW, Gmitter FG Jr, Tusa N, Chandler JL (1990b) Somatic hybrid plants from sexually incompatible woody species: Citrus reticulata and Citropsis gilletiana. Plant Cell Rep 8: 656–659
Hidaka T, Omura M (1992) Regeneration of somatic hybrid plants obtained by electrical fusion between satsuma mandarin (Citrus unshiu) and rough lemon (C. jambhiri) or yuzu (C junos). Jpn J Breed 42: 79–89
Kobayashi S (1987) Uniformity of plants regenerated from orange (Citrus sinensis Osb.) protoplasts. Theor Appl Genet 74: 10–14
Kobayashi S, Ohgawara T (1988) Production of somatic hybrid plants through protoplast fusion in Citrus. Jpn Agric Res Q 22(3): 181–188
Kobayashi S, Uchimiya H, Ikeda I (1983) Plant regeneration from Trovita orange protoplasts. Jpn J Breed 33: 119–122
Kobayashi S, Ikeda I, Nakatani M (1984) Induction of nucellar callus from orange (Citrus sinensis Osb.) ovules, and uniformity of regenerated plants, Bull Fruit Tree Res Stn E 5: 43–54
Kobayashi S, Ikeda I, Uchimiya H (1985) Conditions for high frequency embryogenesis from orange (Citrus sinensis Osb.) protoplasts. Plant Cell Tissue Organ Cult 4: 249–259
Kobayashi S, Ohgawara T, Ohgawara E, Oiyama I, Ishii S (1988) A somatic hybrid plant obtained by protoplast fusion between navel orange (Citrus sinensis) and satsuma mandarin (C. unshiu). Plant Cell Tissue Organ Cult 14: 63–69
Kobayashi S, Ohgawara T, Fujiwara K, Oiyama I (1991a) Analysis of cytoplasmic genomes in somatic hybrids between navel orange (Citrus sinensis Osb.) and Murcott tangor. Theor Appl Genet 82: 6–10
Kobayashi S, Oiyama I, Yoshinaga K, Ohgawara T, Ishii S (1991b) Fertility in an intergeneric somatic hybrid plant of Rutaceae. HortScience 26(2): 207
Kochba J, Spiegel-Roy P, Neumann H, Saad S (1982) Effect of carbohydrates on somatic embryogenesis in subcultured nucellar callus of Citrus cultivars. Z Pflanzenphysiol 105: 359–368
Murashige T, Tucker DPH (1969) Growth factor requirements of Citrus tissue culture. In: Chapman H D (ed) Proc 1st Int Citrus Symp, Riverside, vol 3. pp 1155–1161
Ohgawara T, Kobayashi S (1991) Application of protoplast fusion to Citrus breeding. Food Biotechnol 5(2): 169–184
Ohgawara T, Kobayashi S, Ohgawara E, Uchimiya H, Ishii S (1985) Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata. Theor Appl Genet 71: 1–4
Ohgawara T, Kobayashi S, Ishii S, Yoshinaga K, Oyiama I (1989) Somatic hybridization in Citrus: navel orange (C. sinensis Osb.) and grapefruit (C. paradisi macf.). Theor Appl Genet 78: 609–612
Ohgawara T, Kobayashi S, Ishii S, Yoshinaga K, Oiyama I (1991) Fertile fruit trees obtained by somatic hybridization: navel orange (Citrus sinensis) and Troyer citrange (C. sinensis × Poncirus trifoliata). Theor Appl Genet 81: 141–143
Oiyama I (1981) A technique for chromosome observation in root-tip cells of Citrus. Bull Fruit Tree Res Stn D3: 1–7
Oiyama I, Kobayashi S, Yoshinaga K, Ohgawara T, Ishii S (1991) Use of pollen from a somatic hybrid between Citrus and Poncirus in the production of triploids. HortScience 26(8): 1082
Palmer JD, Shields CR (1984) Tripartite structure of the Brassica campestris mitochondrial genome Nature 307: 437–440
Saito W, Ohgawara T, Shimizu J, Ishii S (1991) Acid citrus somatic hybrids between sudachi (Citrus sudachi Hort. ex Shirai) and lime (C. aurantifolia Swing.) produced by electrofusion. Plant Sci 77: 125–130
Saito W, Ohgawara T, Shimizu J, Ishii S, Kobayashi S (1993) Citrus cybrid regeneration following cell fusion between nucellar cells and mesophyll cells. Plant Sci 88: 195–201
Saunt J (1990) Citrus varieties of the world. Sinclair International Limited, Norwich, pp 122–123
Soost RK, Cameron JW (1975) Citrus. In: Janick J, Moore J N (eds). Advances in Fruit Breeding. Purdue University Press, West Lafayette, Ind, pp 507–540
Sugiura M, Shinozaki K, Zaita N, Kusuda M, Kumano M (1986) Clone bank of the tobacco (Nicotiana tabacum) chloroplast genome as a set of overlapping restriction endonuclease fragments: mapping of eleven ribosomal protein genes. Plant Sci 44: 211–216
Takayanagi R, Hidaka T, Omura M (1992) Regeneration of intergeneric somatic hybrids by electrical fusion between Citrus and its Wild relatives: Mexican lime (Citrus aurantifolia) and Java feroniella (Feroniella lucida) or tabog (Swinglea glutinosa). J Jpn Soc Hortic Sci 60: 799–804
Tusa N, Grosser JW, Gmitter FG Jr (1990) Plant regeneration of Valencia sweet orange Femminello lemon, and the interspecific somatic hybrid following protoplast fusion. J Am Soc Hortic Sci 115(6): 1043–1046
Uchimiya H (1982) Somatic hybridization between male sterile Nicotiana tabacum and N. glutinosa through protoplast fusion. Theor Appl Genet 61: 69–72
Uchimiya H, Ohgawara T, Kato H, Akiyama T, Harada H, Sugiura M (1983) Detection of two different nuclear genomes in parasexual hybrids by ribosomal RNA gene analysis. Theor Appl Genet 64: 117–118
Vardi A, Galun E (1988) Recent advance in protoplast culture of horticultural crops: Citrus. Sci Hortic 37: 217–230
Vardi A, Galun E (1989) Isolation and culture of Citrus protoplasts. In: Bajaj YSP (ed) (Biotechnology in agriculture and forestry, vol 8.) Plant protoplasts and genetic engineering I. Springer, Berlin Heidelberg New York, pp 147–159
Vardi A Spiegel-Roy P, Galun E (1982) Plant regeneration from Citrus protoplasts: variability in methodological requirements among cultivars and species. Theor Appl Genet 62: 171–176
Vardi A, Hutchison DJ, Galun E (1986) A protoplast-to-tree system in Microcitrus based on protoplasts derived from a sustained embryonic callus. Plant Cell Rep 5: 412–414
Vardi A, Breiman A, Galun E (1987) Citrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles. Theor Appl Genet 75: 51–58
Vardi A, Arzee-Gonen P, Frydman-Shani A, Bleichman S, Galun E (1989) Protoplast fusion-mediated transfer of organelles from Microcitrus into Citrus and regeneration of novel alloplasmic trees. Theor Appl Genet 78: 741–747
Wakasa K, Kobayashi M, Kamada H (1984) Colony formation from protoplasts of nitrate reductase-deficient rice cell lines. J Plant Physiol 117: 223–231
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Ohgawara, T., Uchimiya, H., Ishii, S., Kobayashi, S. (1994). Somatic Hybridization Between Citrus sinensis and Poncirus trifoliata . In: Bajaj, Y.P.S. (eds) Somatic Hybridization in Crop Improvement I. Biotechnology in Agriculture and Forestry, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57945-5_30
Download citation
DOI: https://doi.org/10.1007/978-3-642-57945-5_30
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-63411-6
Online ISBN: 978-3-642-57945-5
eBook Packages: Springer Book Archive