Summary
Allotetraploid intergeneric somatic hybrid plants between Citrus reticulata Blanco cv. Cleopatra mandarin and Citropsis gilletiana Swing. & M. Kell. (common name Gillet's cherry orange) were regenerated following protoplast fusion. Cleopatra protoplasts were isolated from an ovule-derived embryogenic suspension culture and fused chemically with leaf-derived protoplasts of Citropsis gilletiana. Cleopatra mandarin and somatic hybrid plants were regenerated via somatic embryogenesis. Hybrid plant identification was based on differential leaf morphology, root-tip cell chromosome number, and electrophoretic analyses of phosphoglucose mutase (PGM) and phosphohexose isomerase (PHI) isozyme banding patterns. This is the first somatic hybrid within the Rutaceae reported that does not have Citrus sinensis (sweet orange) as a parent, and the first produced with a commercially important citrus rootstock and a complementary but sexually incompatible, related species.
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Abbreviations
- PGM:
-
phosphoglucose mutase
- PHI:
-
phosphohexose isomerase
- MES:
-
2[N-morpholino] ethane sulfonic acid
- BH3:
-
protoplast culture medium (Grosser and Chandler, 1987)
- PEG:
-
polyethylene glycol
- MT:
-
Murashige and Tucker (1969) basal medium
- NAA:
-
1-naphthaleneacetic acid
- GA3:
-
gibberellic acid
- H+H and EME:
-
citrus embryogenic cell culture media (Grosser and Gmitter, 1990b)
- B:
-
embryo germination medium
- RMAN:
-
rooting medium
References
Cardy BJ, Stuber CW, Goodman MM (1981) Inst Statist Mimeo Serv, No 1317, North Carolina State Univ, Raleigh
Castle WS (1987) In: Rootstocks for fruit crops, Rom CR and Carlson RF (eds), John Wiley & Sons, Inc, Ch 11
Ford HW, Feder WA (1960) Proc Fla State Hort Soc 73: 60–64
Grosser JW, Chandler JL (1987) Scientia Hort 31: 253–257
Grosser JW, Gmitter FG, Jr (1990a) HortScience 25: 147–151
Grosser JW, Gmitter FG, Jr (1990b) Plant Breed Rev, Vol 8 (In press)
Grosser JW, Gmitter FG, Jr (1990c) Proc Hort Biotech Symp (In press)
Grosser JW, Gmitter FG, Jr, Chandler JL (1988a) Plant Cell Rep 7: 5–8
Grosser JW, Gmitter FG, Jr, Chandler JL (1988b) Theor Appl Genet 75: 397–401
Grosser JW, Moore GA, Gmitter FG, Jr (1989) Scientia Hort 39: 23–29
Menczel LF, Nagy F, Kiss Z, Maliga P (1981) Theor Appl Genet 59: 191–195
Murashige T, Tucker DPH (1969) Proc 1st Int Citrus Symp 3: 1155–1161
Moore GA, Castle WS (1988) J Hered 79: 59–63
Ochatt SJ, Patat-Ochatt EM, Rech EL, Davey MR, Power JB (1989) Theor Appl Genet 78: 35–41
Sass JE (1958) In: Botanical microtechnique, Iowa State Univ Press, pp 73–75
Soost RK, Torres AM (1981) Proc Int Soc Citriculture 1: 7–10
Swingle WT, Reece PC (1967) In: The Citrus Ind, Vol 1 Reuther W, Batchelor LD, Webber HJ (eds), Univ. of California Press, Berkeley, Ch 3
Torres AM, Soost RK, Diedenhofen U (1978) Am J Bot 65: 869–881
Vallejos CE (1983) In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, Part A, Elsevier, Amsterdam, pp 469–516
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Communicated by G.C. Phillips
Florida Agricultural Experiment Station Journal Series No. R-00298.
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Grosser, J.W., Gmitter, F.G., Tusa, N. et al. Somatic hybrid plants from sexually incompatible woody species: Citrus reticulata and Citropsis gilletiana . Plant Cell Reports 8, 656–659 (1990). https://doi.org/10.1007/BF00269986
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DOI: https://doi.org/10.1007/BF00269986