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Production and Genetic Engineering of Terpenoids Production in Plant Cell and Organ Cultures

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Natural Products

Abstract

Among the three main groups of secondary metabolites that can be found in plants, terpenes are by far the largest and most diverse. They play an important role in plants in both primary and secondary metabolism and constitute a source of phytochemicals for human health. Plant biotechnology, based on in vitro culture and genetic engineering techniques, has proved to be a promising tool to increase the production of these bioactive compounds. This chapter describes the plant cell and organ culture techniques used in plant biotechnology, the biotechnological production of the different groups of terpenes using empirical approaches and some examples of genetic and metabolic engineering techniques applied to improve the production of these valuable plant secondary metabolites in plant cell and organ cultures.

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Abbreviations

CaMV:

Cauliflower mosaic virus 35S promoter

CM:

Culture medium

DMAPP:

Dimethylallyl diphosphate

DXR:

1-Deoxy-D-xylulose-5-phosphate reductoisomerase

DXS:

1-Deoxy-D-xylulose-5-phosphate synthase

DW:

Dry weight

FPP:

Farnesyl diphosphate

GGPP:

Geranylgeranyl diphosphate

GPP:

Geranyl diphosphate

HMGR:

3-Hydroxy-3-methyl-glutaryl-CoA reductase

IAA:

Indole-3-acetic acid

IPP:

Isopentenyl diphosphate

MEP:

2-C-methyl-D-erythritol 4-phosphate

MS:

Murashige and Skoog medium

OPP:

Diphosphate

PGR:

Plant Growth Regulators

SQS:

Squalene synthase

TXS:

Taxadiene synthase

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Acknowledgments

Work in the Plant Physiology Laboratory (University of Barcelona) was financially supported by the Spanish MEC (BIO2008-01210, BIO2011-29856-C02-01) and the Generalitat de Catalunya (2009SGR1217).

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Authors and Affiliations

  1. Laboratori de Fisiologia Vegetal, Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII s/n, Barcelona, 08028, Spain

    Dr. Mercedes Bonfill, Rosa Cusido & Javier Palazón

  2. Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, 6109, 13083-970, Campinas, Brazil

    Sonia Malik

  3. Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, 1983963113, Tehran, Iran

    M. Hossein Mirjalili

  4. CEPROCOR Science and Technology Ministery, Còrdoba, Argentina

    Marta Goleniowski

Authors
  1. Dr. Mercedes Bonfill
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  2. Sonia Malik
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  3. M. Hossein Mirjalili
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  4. Marta Goleniowski
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  5. Rosa Cusido
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  6. Javier Palazón
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Corresponding author

Correspondence to Mercedes Bonfill .

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Editors and Affiliations

  1. Botany Department, M.L. Sukhadia University, Durga Nursery Road, Udaipur, 313001, Rajasthan, India

    Kishan Gopal Ramawat

  2. Institute of Vine and Wine Sciences, Biological-Active Plant Substances Study, University of Bordeaux, 210 Chemin de Leysotte CS 50008, Villenave d'Ornon, 33882, France

    Jean-Michel Mérillon

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Bonfill, M., Malik, S., Mirjalili, M.H., Goleniowski, M., Cusido, R., Palazón, J. (2013). Production and Genetic Engineering of Terpenoids Production in Plant Cell and Organ Cultures. In: Ramawat, K., Mérillon, JM. (eds) Natural Products. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22144-6_123

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