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Current Technologies Based on the Knowledge of the Stem Cells Microenvironments

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Stem Cell Microenvironments and Beyond

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1041))

Abstract

The stem cell microenvironment or niche plays a critical role in the regulation of survival, differentiation and behavior of stem cells and their progenies. Recapitulating each aspect of the stem cell niche is therefore essential for their optimal use in in vitro studies and in vivo as future therapeutics in humans. Engineering of optimal conditions for three-dimensional stem cell culture includes multiple transient and dynamic physiological stimuli, such as blood flow and tissue stiffness. Bioprinting and microfluidics technologies, including organs-on-a-chip, are among the most recent approaches utilized to replicate the three-dimensional stem cell niche for human tissue fabrication that allow the integration of multiple levels of tissue complexity, including blood flow. This chapter focuses on the physico-chemical and genetic cues utilized to engineer the stem cell niche and provides an overview on how both bioprinting and microfluidics technologies are improving our knowledge in this field for both disease modeling and tissue regeneration, including drug discovery and toxicity high-throughput assays and stem cell-based therapies in humans.

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Authors and Affiliations

  1. Faculty of Science, School of Materials Science and Engineering, University of New South Wales, Sydney, NSW, Australia

    Damia Mawad

  2. Sydney Medical School, University of Sydney, Sydney, NSW, Australia

    Gemma Figtree & Carmine Gentile

  3. Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

    Carmine Gentile

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  1. Damia Mawad
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  2. Gemma Figtree
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  3. Carmine Gentile
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Correspondence to Carmine Gentile .

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  1. Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Alexander Birbrair

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Mawad, D., Figtree, G., Gentile, C. (2017). Current Technologies Based on the Knowledge of the Stem Cells Microenvironments. In: Birbrair, A. (eds) Stem Cell Microenvironments and Beyond. Advances in Experimental Medicine and Biology, vol 1041. Springer, Cham. https://doi.org/10.1007/978-3-319-69194-7_13

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