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Selection of Recombinant Human Antibodies

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Protein Targeting Compounds

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 917))

Abstract

Since the development of therapeutic antibodies the demand of recombinant human antibodies is steadily increasing. Traditionally, therapeutic antibodies were generated by immunization of rat or mice, the generation of hybridoma clones, cloning of the antibody genes and subsequent humanization and engineering of the lead candidates. In the last few years, techniques were developed that use transgenic animals with a human antibody gene repertoire. Here, modern recombinant DNA technologies can be combined with well established immunization and hybridoma technologies to generate already affinity maturated human antibodies. An alternative are in vitro technologies which enabled the generation of fully human antibodies from antibody gene libraries that even exceed the human antibody repertoire. Specific antibodies can be isolated from these libraries in a very short time and therefore reduce the development time of an antibody drug at a very early stage.

In this review, we describe different technologies that are currently used for the in vitro and in vivo generation of human antibodies.

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Acknowledgements

We gratefully acknowledge the financial support by the FP7 collaborative projects AffinityProteome (contract 222635) and AFFINOMICS (contract 241481). (contract 222635) and AFFINOMICS (contract 241481). Parts of this article are updated and revised version of [39, 40, 150].

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  1. Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany

    Florian Tomszak, Susanne Weber, Jonas Zantow, Thomas Schirrmann, Michael Hust & André Frenzel

  2. YUMAB GmbH, Rebenring 33, 38106, Braunschweig, Germany

    Florian Tomszak, Thomas Schirrmann & André Frenzel

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  1. Florian Tomszak
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  2. Susanne Weber
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  3. Jonas Zantow
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  4. Thomas Schirrmann
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  5. Michael Hust
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  6. André Frenzel
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Correspondence to André Frenzel .

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  1. Recombinant protein exprsn/Intrabdy unit, Helmholtz-Centre for Infection Rese, Braunschweig, Germany

    Thomas Böldicke

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Tomszak, F., Weber, S., Zantow, J., Schirrmann, T., Hust, M., Frenzel, A. (2016). Selection of Recombinant Human Antibodies. In: Böldicke, T. (eds) Protein Targeting Compounds. Advances in Experimental Medicine and Biology, vol 917. Springer, Cham. https://doi.org/10.1007/978-3-319-32805-8_3

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