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Strategies and Methodologies for the Co-expression of Multiple Proteins in Plants

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Advanced Technologies for Protein Complex Production and Characterization

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 896))

Abstract

The first transgenes were introduced in a plant genome more than 30 years ago. Since then, the capabilities of the plant scientific community to engineer the genome of plants have progressed at an unparalleled speed. Plant genetic engineering has become a central technology that has dramatically incremented our basic knowledge of plant biology and has enabled the translation of this knowledge into a number of increasingly complex and sophisticated biotechnological applications, which in most cases rely on the simultaneous co-expression of multiple recombinant proteins from different origins. To meet the new challenges of modern plant biotechnology, the plant scientific community has developed a vast arsenal of innovative molecular tools and genome engineering strategies. In this chapter we review a variety of tools, technologies, and strategies developed to transfer and simultaneously co-express multiple transgenes and proteins in a plant host. Their potential advantages, disadvantages, and future prospects are also discussed.

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Acknowledgments

We gratefully acknowledge the support of the Spanish Ministerio de Economía y Competitividad (AGL2013-43522-R) and the Generalitat de Catalunya (2014 SGR 1434).

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Authors and Affiliations

  1. Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra-Cerdanyola del Vallès, 08193, Barcelona, Spain

    Albert Ferrer, Monserrat Arró, David Manzano & Teresa Altabella

  2. Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, 08028, Barcelona, Spain

    Albert Ferrer, Monserrat Arró & David Manzano

  3. Department of Plant Physiology, Faculty of Pharmacy, University of Barcelona, 08028, Barcelona, Spain

    Teresa Altabella

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  1. Albert Ferrer
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  2. Monserrat Arró
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  4. Teresa Altabella
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Correspondence to Albert Ferrer .

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  1. Center for Biological Research, SpanishNationalResearchCouncil(CIB-CSIC), Madrid, Spain

    M. Cristina Vega

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Ferrer, A., Arró, M., Manzano, D., Altabella, T. (2016). Strategies and Methodologies for the Co-expression of Multiple Proteins in Plants. In: Vega, M. (eds) Advanced Technologies for Protein Complex Production and Characterization. Advances in Experimental Medicine and Biology, vol 896. Springer, Cham. https://doi.org/10.1007/978-3-319-27216-0_17

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