Abstract
Leuconostoc spp. are gram-positive and heterofermentative bacteria, which are capable of transforming glucose molecules into carbon dioxide, ethanol, and lactate. Leuconostoc spp. exist in vegetables, silage, fermented food products, and feces, among other places. These bacteria are used as a starter culture in food and beverage fermentation in order to improve the nutritional and organoleptic quality and to extend shelf life. They produce exo-polysaccharides (dextran or levan), oligosaccharides, mannitol, bacteriocins, and vitamins. In this chapter, we present an extensive discussion on Leuconostoc spp., especially general information including morphology, taxonomy, growth characteristics, metabolism, starter uses in fermented foods, and beneficial health effects as potential probiotics.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
AFRC RF (1989) Probiotics in man and animals. J Appl Bacteriol 66(5):365–378
Agarwal K, Bhasin S (2002) Feasibility studies to control acute diarrhoea in children by feeding fermented milk preparations Actimel and Indian Dahi. Eur J Clin Nutr 56:S56–S59
Ahn G-H, Moon JS, Shin S-Y, Min WK, Han NS, Seo J-H (2015) A competitive quantitative polymerase chain reaction method for characterizing the population dynamics during kimchi fermentation. J Ind Microbiol Biotechnol 42:49–55
Auge B, Donnio P, Le Deaut P, Avril J (1987) Influence of vancomycin by venous route on salivary and fecal aerobic floras. Pathol Biol 35(5):673–675
Benmechernene Z, Chentouf HF, Yahia B, Fatima G, Quintela-Baluja M, Calo-Mata P, Barros-Velázquez J (2013) Technological aptitude and applications of Leuconostoc mesenteroides bioactive strains isolated from algerian raw camel milk. BioMed Res Int. doi:10.1155/2013/418132
Björkroth J, Holzapfel W (2006) Genera Leuconostoc, Oenococcus and Weissella. In: The prokaryotes. Springer, Berlin, pp 267–319
Bozonnet S, Dols-Laffargue M, Fabre E, Pizzut S, Remaud-Simeon M, Monsan P, Willemot R-M (2002) Molecular characterization of DSR-E, an α-1, 2 linkage-synthesizing dextransucrase with two catalytic domains. J Bacteriol 184(20):5753–5761
Budde BB, Hornbaek T, Jacobsen T, Barkholt V, Koch AG (2003) Leuconostoc carnosum 4010 has the potential for use as a protective culture for vacuum-packed meats: culture isolation, bacteriocin identification, and meat application experiments. Int J Food Microbiol 83(2):171–184
Caplice E, Fitzgerald GF (1999) Food fermentations: role of microorganisms in food production and preservation. Int J Food Microbiol 50(1):131–149
Carr FJ, Chill D, Maida N (2002) The lactic acid bacteria: a literature survey. Crit Rev Microbiol 28(4):281–370
Carvalheiro F, Moniz P, Duarte LC, Esteves MP, Gírio FM (2011) Mannitol production by lactic acid bacteria grown in supplemented carob syrup. J Ind Microbiol Biotechnol 38(1):221–227
Cho SK, Eom H-J, Moon JS, Lim S-B, Kim YK, Lee KW, Han NS (2014) An improved process of isomaltooligosaccharide production in kimchi involving the addition of a Leuconostoc starter and sugars. Int J Food Microbiol 170:61–64
Choi H, Kim Y-W, Hwang I, Kim J, Yoon S (2012) Evaluation of Leuconostoc citreum HO12 and Weissella koreensis HO20 isolated from kimchi as a starter culture for whole wheat sourdough. Food Chem 134(4):2208–2216
Cogan TM, Jordan KN (1994) Metabolism of Leuconostoc bacteria. J Dairy Sci 77(9):2704–2717
Collins M, Samelis J, Metaxopoulos J, Wallbanks S (1993) Taxonomic studies on some Leuconostoc‐like organisms from fermented sausages: description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. J Appl Bacteriol 75(6):595–603
Condon S (1987) Responses of lactic acid bacteria to oxygen. FEMS Microbiol Lett 46(3):269–280
Dal Bello F, Walter J, Hammes W, Hertel C (2003) Increased complexity of the species composition of lactic acid bacteria in human feces revealed by alternative incubation condition. Microb Ecol 45(4):455–463
Devoyod J, POULLAIN F (1988) Les Leuconostocs. Propriétés: leur rôle en technologie laitière. Lait 68(3):249–279
Dicks L, Dellaglio F, Collins M (1995) Proposal to reclassify Leuconostoc oenos as Oenococcus oeni [corrig.] gen. nov., comb. nov. Int J Syst Bacteriol 45(2):395–397
Drosinos EH, Mataragas M, Xiraphi N, Moschonas G, Gaitis F, Metaxopoulos J (2005) Characterization of the microbial flora from a traditional Greek fermented sausage. Meat Sci 69(2):307–317
Duboc P, Mollet B (2001) Applications of exopolysaccharides in the dairy industry. Int Dairy J 11(9):759–768
Ennahar S, Cai Y, Fujita Y (2003) Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis. Appl Environ Microbiol 69(1):444–451
Eom H-J, Seo DM, Han NS (2007) Selection of psychrotrophic Leuconostoc spp. producing highly active dextransucrase from lactate fermented vegetables. Int J Food Microbiol 117:61–67
Eom H-J, Park JM, Seo MJ, Kim MD, Han NS (2008) Monitoring of Leuconostoc mesenteroides DRC starter in fermented vegetable by random integration of chloramphenicol acetyltransferase gene. J Ind Microbiol Biotechnol 35(9):953–959
Euzéby JP (1997) List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47(2):590–592
FAO/WHO (1974) Toxicological evaluation of certain food additives with a review of general principles and of specifications. Seventeenth report of the Joint FAO-WHO Expert Committee on Food Additives. WHO Technical Report Series, vol 539. WHO, pp 1–40
FAO/WHO (2002) Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food. FAO/WHO, Rome
Foucaud C, Francois A, Richard J (1997) Development of a chemically defined medium for the growth of Leuconostoc mesenteroides. Appl Environ Microbiol 63(1):301–304
Galle S, Schwab C, Arendt E, Ganzle M (2010) Exopolysaccharide-forming Weissella strains as starter cultures for sorghum and wheat sourdoughs. J Agric Food Chem 58(9):5834–5841
Gänzle M, Schwab C (2009) Ecology of exopolysaccharide formation by lactic acid bacteria: sucrose utilization, stress tolerance, and biofilm formation. In: Ullrich M (ed) Bacterial polysaccharides: current innovations and future trends. Caister Academic, Norfolk, pp 263–278
Garvie E (1967) The growth factor and amino acid requirements of species of the genus Leuconostoc, including Leuconostoc paramesenteroides (sp. nov.) and Leuconostoc oenos. J Gen Microbiol 48(3):439–447
Garvie E (1986) Genus Leuconostoc. In: Garrity GM (ed) Bergey’s manual of systematic bacteriology, vol 2. William and Wilkins, Baltimore, pp 1071–1075
Gavett SH, O’Hearn DJ, Li X, Huang SK, Findelman FD, Wills-Karp M (1995) Interleukin 12 inhibits antigen-induced airway hyperresponsiveness, inflammation, and Th2 cytokine expression in mice. J Exp Med 182:1527–1536
Ghoreishi S, Shahrestani R (2009) Subcritical water extraction of mannitol from olive leaves. J Food Eng 93(4):474–481
Granato D, Branco GF, Nazzaro F, Cruz AG, Faria JA (2010) Functional foods and nondairy probiotic food development: trends, concepts, and products. Comp Rev Food Sci Food Saf 9(3):292–302
Greppi A, Ferrocino I, La Storia A, Rantsiou K, Ercolini D, Cocolin L (2015) Monitoring of the microbiota of fermented sausages by culture independent rRNA-based approaches. Int J Food Microbiol. doi:10.1016/j.ijfoodmicro.2015.01.016
Gueguen Y, Chemardin P, Labrot P, Arnaud A, Galzy P (1997) Purification and characterization of an intracellular β‐glucosidase from a new strain of Leuconostoc mesenteroides isolated from cassava. J Appl Microbiol 82(4):469–476
Hamasaki Y, Ayaki M, Fuchu H, Sugiyama M, Morita H (2003) Behavior of psychrotrophic lactic acid bacteria isolated from spoiling cooked meat products. Appl Environ Microbiol 69(6):3668–3671
Han H, Lim C-R, Park H-K (1990) Determination of microbial community as an indicator of kimchi fermentation. Korean J Food Sci Technol 22(1):26–32
Hastings JW, Stiles ME, von Holy A (1994) Bacteriocins of Leuconostocs isolated from meat. Int J Food Microbiol 24(1):75–81
Héchard Y, Berjeaud J-M, Cenatiempo Y (1999) Characterization of the mesB gene and expression of bacteriocins by Leuconostoc mesenteroides Y105. Curr Microbiol 39(5):265–269
Hemme D, Foucaud-Scheunemann C (2004) Leuconostoc, characteristics, use in dairy technology and prospects in functional foods. Int Dairy J 14(6):467–494
Holt S, Al‐Sheikh H, Shin KJ (2001) Characterization of dextran‐producing Leuconostoc strains. Lett Appl Microbiol 32(3):185–189
Jin Q, Yoon H-S, Han NS, Lee J, Han JS (2006) Effect of lactic acid bacteria on D-and L-lactic acid contents of kimchi. Food Sci Biotechnol 15(6):948–953
Jin Q, Jung JY, Kim YJ, Eom HJ, Kim SY, Kim TJ, Han NS (2009) Production of L-lactate in Leuconostoc citreum via heterologous expression of L-lactate dehydrogenase gene. J Biotechnol 144(2):160–164
Jin Q, Li L, Kim YJ, Han NS (2014) Construction of a dextran-free Leuconostoc citreum mutant by targeted destruction of the dextransucrase gene. J Appl Microbiol 117:1104–1112
Johanningsmeier S, McFeeters RF, Fleming HP, Thompson RL (2007) Effects of Leuconostoc mesenteroides starter culture on fermentation of cabbage with reduced salt concentrations. J Food Sci 72(5):M166–M172
Jung JY, Lee SH, Lee HJ, Seo HY, Park WS, Jeon CO (2012) Effects of Leuconostoc mesenteroides starter cultures on microbial communities and metabolites during kimchi fermentation. Int J Food Microbiol 153(3):378–387
Jung JY, Lee SH, Jeon CO (2014) Kimchi microflora: history, current status, and perspectives for industrial kimchi production. Appl Microbiol Biotechnol 98(6):2385–2393
Kang H, Oh YJ, Ahn KS, Eom HJ, Han N, Kim YB, Sohn NW (2009) Leuconostoc citreum HJ-P4 (KACC 91035) regulates immunoglobulin E in an ovalbumin-induced allergy model and induces interleukin-12 through nuclear factor-kappa B and p38/c-Jun N-terminal kinases signaling in macrophages. Microbiol Immunol 53(6):331–339
Kang HK, Yun SI, Lim TY, Xia Y-M, Kim D (2011) Cloning of levansucrase from Leuconostoc mesenteroides and its expression in Pichia pastoris. Food Sci Biotechnol 20(1):277–281
Keenan T (1968) Production of acetic acid and other volatile compounds by Leuconostoc citrovorum and Leuconostoc dextranicum. Appl Microbiol 16(12):1881–1885
Kekkonen RA, Kajasto E, Miettinen M, Veckman V, Korpela R, Julkunen I (2008) Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production. World J Gastroenterol 14(8):1192
Kim JE, Eom H-J, Kim Y, Ahn JE, Kim JH, Han NS (2012a) Enhancing acid tolerance of Leuconostoc mesenteroides with glutathione. Biotechnol Lett 34:683–687
Kim YJ, Eom H-J, Seo E-Y, Lee DY, Kim JH, Han NS (2012b) Development a chemically defined minimal medium for the exponential growth of Leuconostoc mesenteroides ATCC8293. J Microbiol Biotechnol 22(11):1518–1522
Kim JE, Eom H-J, Li L, Yoo K-S, Han NS (2014) Induction of the acid tolerance response in Leuconostoc mesenteroides ATCC 8293 by pre-adaptation in acidic condition. Food Sci Biotechnol 23(1):221–224
Klaenhammer TR (1993) Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev 12(1‐3):39–85
Kuipers OP, Buist G, Kok J (2000) Current strategies for improving food bacteria. Res Microbiol 151(10):815–822
LeBlanc J, Laiño J, del Valle MJ, Vannini V, Van Sinderen D, Taranto M, de Valdez G, de Giori GS, Sesma F (2011) B‐Group vitamin production by lactic acid bacteria–current knowledge and potential applications. J Appl Microbiol 111(6):1297–1309
Leroy F, De Vuyst L (2004) Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends Food Sci Technol 15(2):67–78
Levata-Jovanovic M, Sandine WE (1997) A method to use Leuconostoc mesenteroides ssp. cremoris 91404 to improve milk fermentations. J Dairy Sci 80(1):11–18
Li L, Shin S-Y, Lee KW, Han NS (2014) Production of natural antimicrobial compound D-phenyllactic acid using Leuconostoc mesenteroides ATCC 8293 whole cells involving highly active D-lactate dehydrogenase. Lett Appl Microbiol 59:404–411
Martinez-Murcia A, Collins M (1991) A phylogenetic analysis of an atypical Leuconostoc: description of Leuconostoc fallax sp. nov. FEMS Microbiol Lett 82(1):55–59
Martley F, Crow V (1993) Interactions between non-starter microorganisms during cheese manufacture and repening. Int Dairy J 3(4):461–483
Masuda Y, Ono H, Kitagawa H, Ito H, Mu F, Sawa N, Zendo T, Sonomoto K (2011) Identification and characterization of leucocyclicin Q, a novel cyclic bacteriocin produced by Leuconostoc mesenteroides TK41401. Appl Environ Microbiol 77(22):8164–8170
McSweeney PL, Sousa MJ (2000) Biochemical pathways for the production of flavour compounds in cheeses during ripening: A review. Lait 80(3):293–324
Monchois V, Willemot RM, Monsan P (1999) Glucansucrases: mechanism of action and structure–function relationships. FEMS Microbiol Rev 23(2):131–151
Monedero V, Pérez-Martínez G, Yebra MJ (2010) Perspectives of engineering lactic acid bacteria for biotechnological polyol production. Appl Microbiol Biotechnol 86(4):1003–1015
Morishita T, Tamura N, Makino T, Kudo S (1999) Production of menaquinones by lactic acid bacteria. J Dairy Sci 82(9):1897–1903
Nissen-Meyer J, Oppegård C, Rogne P, Haugen HS, Kristiansen PE (2010) Structure and mode-of-action of the two-peptide (class-IIb) bacteriocins. Probiotics Antimicrob Proteins 2(1):52–60
Olson RE (1984) The function and metabolism of vitamin K. Annu Rev Nutr 4(1):281–337
Otgonbayar GE, Eom HJ, Kim BS, Ko JH, Han NS (2011) Mannitol production by Leuconostoc citreum KACC 91348P isolated from Kimchi. J Microbiol Biotechnol 21(9):968–971
Papathanasopoulos MA, Krier F, Revol-Junelles A-M, Lefebvre G, Le Caer JP, von Holy A, Hastings JW (1997) Multiple bacteriocin production by Leuconostoc mesenteroides TA33a and other Leuconostoc/Weissella strains. Curr Microbiol 35(6):331–335
Patra F, Tomar S, Arora S (2009) Technological and functional applications of low‐calorie sweeteners from lactic acid bacteria. J Food Sci 74(1):R16–R23
Plengvidhya V, Breidt F, Lu Z, Fleming HP (2007) DNA fingerprinting of lactic acid bacteria in sauerkraut fermentations. Appl Environ Microbiol 73(23):7697–7702
Rhee SK, Song KB, Kim CH, Park BS, Jang EK, Jang KH (2002) Levan. In: Baets S, Vandamme EJ, Steinbuchel A (eds) Biopolymers, polysaccharides I: Polysaccharides from prokaryotes, vol V. Wiley-VCH, Weinheim, pp 351–377
Sanchez JI, Martinez B, Rodriguez A (2005) Rational selection of Leuconostoc strains for mixed starters based on the physiological biodiversity found in raw milk fermentations. Int J Microbiol 105(3):377–387
Sandine W (1996) Commercial production of dairy starter cultures. VCH, New York
Sanz ML, Cote GL, Gibson GR, Rastall RA (2005) Prebiotic properties of alternansucrase maltose-acceptor oligosaccharides. J Agric Food Chem 53(15):5911–5916
Sarbini SR, Kolida S, Naeye T, Einenhand AW, Gibson GR, Rastall RA (2013) The prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system. J Funct Foods 5(4):1938–1946
Schmitt P, Vasseur C, Phalip V, Huang D, Divies C, Prevost H (1997) Diacetyl and acetoin production from the co-metabolism of citrate and xylose by Leuconostoc mesenteroides subsp. mesenteroides. Appl Microbiol Biotechnol 47(6):715–718
Seo DM, Kim S-Y, Eom H-J, Han NS (2007) Synbiotic synthesis of oligosaccharides during milk fermentation by addition of Leuconostoc starter and sugars. J Microbiol Biotechnol 17(11):1758–1764
Server-busson C, Foucaud C, Leveay J-Y (1999) Selection of dairy Leuconostoc isolates for important technological properties. J Dairy Res 66(02):245–256
Servin AL, Coconnier M-H (2003) Adhesion of probiotic strains to the intestinal mucosa and interaction with pathogens. Best Pract Res Clin Gastroenterol 17(5):741–754
Sneath PH, Mair N, Sharpe M, Holt J (1986) Bergey’s manual of systematic bacteriology. Williams and Wilkins, Baltimore, p 9
Soetaert W (1990) Production of mannitol with Leuconostoc mesenteroides. Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent 55(4):1549–1552
Speckman R, Collins E (1968) Diacetyl biosynthesis in Streptococcus diacetilactis and Leuconostoc citrovorum. J Bacteriol 95(1):174–180
Stiles ME, Holzapfel WH (1997) Lactic acid bacteria of foods and their current taxonomy. Int J Food Microbiol 36(1):1–29
Suvarna V, Boby V (2005) Probiotics in human health: a current assessment. Curr Sci 88(11):1744–1748
Sybesma W, Starrenburg M, Tijsseling L, Hoefnagel MH, Hugenholtz J (2003) Effects of cultivation conditions on folate production by lactic acid bacteria. Appl Environ Microbiol 69(8):4542–4548
Thunell R (1995) Taxonomy of the Leuconostocs. J Dairy Sci 78(11):2514–2522
Todorov SD, Dicks LM (2004) Characterization of mesentericin ST99, a bacteriocin produced by Leuconostoc mesenteroides subsp. dextranicum ST99 isolated from boza. J Ind Microbiol Biotechnol 31(7):323–329
Tolonen M, Taipale M, Viander B, Pihlava JM, Korhonen H, Ryhanen EL (2002) Plant-derived biomolecules in fermented cabbage. J Agric Food Chem 50(23):6798–6803
von Weymarn N, Hujanen M, Leisola M (2002) Production of D-mannitol by heterofermentative lactic acid bacteria. Process Biochem 37(11):1207–1213
Vuyst L, Degeest B (1999) Heteropolysaccharides from lactic acid bacteria. FEMS Microbiol Rev 23(2):153–177
Wiander B, Ryhänen E-L (2005) Laboratory and large-scale fermentation of white cabbage into sauerkraut and sauerkraut juice by using starters in combination with mineral salt with a low NaCl content. Eur Food Res Technol 220(2):191–195
Zhang W, Liu M, Dai X (2013) Biological characteristics and probiotic effect of Leuconostoc lactis strain isolated from the intestine of black porgy fish. Braz J Microbiol 44(3):685–691
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer International Publishing Switzerland
About this chapter
Cite this chapter
Shin, SY., Han, N.S. (2015). Leuconostoc spp. as Starters and Their Beneficial Roles in Fermented Foods. In: Liong, MT. (eds) Beneficial Microorganisms in Food and Nutraceuticals. Microbiology Monographs, vol 27. Springer, Cham. https://doi.org/10.1007/978-3-319-23177-8_5
Download citation
DOI: https://doi.org/10.1007/978-3-319-23177-8_5
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-23176-1
Online ISBN: 978-3-319-23177-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)