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Preparation of Human Cerebrospinal Fluid for Proteomics Biomarker Analysis

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Proteomics for Biomarker Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1002))

Abstract

The analysis of the cerebrospinal fluid (CSF) proteome in recent years has resulted in a valuable repository of data for targeting and diagnosing a variety of diseases, such as Parkinson’s disease, Alzheimer’s disease, traumatic brain injury, and amyotrophic lateral sclerosis. Human ventricular CSF contains numerous proteins that are unique to CSF due in part to the interaction of the biofluid with the brain. This allows researchers to obtain information from a region that would otherwise be inaccessible except through invasive surgery or during autopsy. Characterization of the CSF proteome requires that strict care be taken so that sample integrity and fidelity are maintained to ensure data reproducibility. Standardized methods in sample collection, storage, preparation, analysis, and data mining must be used for meaningful information to be obtained. The following method describes a simple and robust approach for preparing CSF samples for analysis via reversed-phase liquid chromatography (RPLC) and mass spectrometry (MS).

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Acknowledgments

This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under Contract HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the United States Government.

Author information

Authors and Affiliations

  1. Frederick National Laboratory for Cancer Research, Frederick, MD, USA

    Timothy J. Waybright

Authors
  1. Timothy J. Waybright
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Editor information

Editors and Affiliations

  1. , Laboratory of Proteomics and Analytical, Frederick National Laboratory for Cancer, N/A, Frederick, 21702-1201, Maryland, USA

    Ming Zhou

  2. , Laboratory of Proteomics and Analytical, Frederick National Laboratory for Cancer, N/A 5540, Frederick, 21702-1201, Maryland, USA

    Timothy Veenstra

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Waybright, T.J. (2013). Preparation of Human Cerebrospinal Fluid for Proteomics Biomarker Analysis. In: Zhou, M., Veenstra, T. (eds) Proteomics for Biomarker Discovery. Methods in Molecular Biology, vol 1002. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-360-2_5

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  • DOI: https://doi.org/10.1007/978-1-62703-360-2_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-359-6

  • Online ISBN: 978-1-62703-360-2

  • eBook Packages: Springer Protocols

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