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Proteomics for Biomarker Discovery

  • Ming Zhou
  • Timothy Veenstra

Part of the Methods in Molecular Biology book series (MIMB, volume 1002)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Julian A. J. Jaros, Paul C. Guest, Sabine Bahn, Daniel Martins-de-Souza
    Pages 1-11
  3. Yoshiyuki Suehara, Daisuke Kubota, Tsuyoshi Saito
    Pages 13-23
  4. Valeria Severino, Annarita Farina, Angela Chambery
    Pages 37-60
  5. Sumana Mukherjee, Jaime Rodriguez-Canales, Jeffrey Hanson, Michael R. Emmert-Buck, Michael A. Tangrea, DaRue A. Prieto et al.
    Pages 71-83
  6. Leroi V. DeSouza, Sébastien N. Voisin, K. W. Michael Siu
    Pages 105-114
  7. Jintang He, Yashu Liu, Jing Wu, David M. Lubman
    Pages 115-122
  8. Benjamin C. Orsburn
    Pages 123-131
  9. Xiaoying Ye, King C. Chan, DaRue A. Prieto, Brian T. Luke, Donald J. Johann Jr., Luke H. Stockwin et al.
    Pages 133-149
  10. Jerry D. Holman, Surendra Dasari, David L. Tabb
    Pages 167-179
  11. Zhaojing Meng, Apurva K. Srivastava, Ming Zhou, Timothy Veenstra
    Pages 181-193
  12. Timothy J. Waybright, Xia Xu, Jessica M. Faupel-Badger, Zhen Xiao
    Pages 195-203
  13. Karlie A. Neilson, Tim Keighley, Dana Pascovici, Brett Cooke, Paul A. Haynes
    Pages 205-222
  14. Liqian Gao, Hongyan Sun, Mahesh Uttamchandani, Shao Q. Yao
    Pages 233-251
  15. Simona Colantonio, Roberta M. Smith
    Pages 253-265
  16. Brett Chapman, Natalie Castellana, Alex Apffel, Ryan Ghan, Grant R. Cramer, Matthew Bellgard et al.
    Pages 267-294
  17. Scott P. Goulding, Michael J. MacCoss, Christine C. Wu
    Pages 295-309
  18. Scott P. Goulding, Michael J. MacCoss, Christine C. Wu
    Pages E1-E1
  19. Back Matter
    Pages 317-320

About this book

Introduction

With the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures.  While the mass spectrometer resides at the heart of proteomics, its ability to characterize biological samples is only as good as the sample preparation and data analysis tools used in any study.  In Proteomics for Biomarker Discovery, expert researchers in the field detail many of the methods which are now commonly used to study proteomics. These methods and techniques include both label-free approaches and those that utilize stable isotopes incorporated both during cell growth or added via a chemical reaction once the proteome is extracted from the cell. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Proteomics for Biomarker Discovery seeks to aid scientists in the further study of the different sample preparation and data analysis tools used in proteomics today.

Keywords

cell growth cells chemical reaction label-free approaches mass spectrometry measuring post-translational modifications phosphorylation and glycosylation proteomics quantitate protein levels stable isotopes

Editors and affiliations

  • Ming Zhou
    • 1
  • Timothy Veenstra
    • 2
  1. 1., Laboratory of Proteomics and AnalyticalFrederick National Laboratory for CancerFrederickUSA
  2. 2., Laboratory of Proteomics and AnalyticalFrederick National Laboratory for CancerFrederickUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-360-2
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-359-6
  • Online ISBN 978-1-62703-360-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site