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Plasmid DNA Production for Therapeutic Applications

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Recombinant Gene Expression

Part of the book series: Methods in Molecular Biology ((MIMB,volume 824))

Abstract

Plasmid DNA (pDNA) is the base for promising DNA vaccines and gene therapies against many infectious, acquired, and genetic diseases, including HIV-AIDS, Ebola, Malaria, and different types of cancer, enteric pathogens, and influenza. Compared to conventional vaccines, DNA vaccines have many advantages such as high stability, not being infectious, focusing the immune response to only those antigens desired for immunization and long-term persistence of the vaccine protection. Especially in developing countries, where conventional effective vaccines are often unavailable or too expensive, there is a need for both new and improved vaccines. Therefore the demand of pDNA is expected to rise significantly in the near future. Since the injection of pDNA usually only leads to a weak immune response, several milligrams of DNA vaccine are necessary for immunization protection. Hence, there is a special interest to raise the product yield in order to reduce manufacturing costs. In this chapter, the different stages of plasmid DNA production are reviewed, from the vector design to downstream operation options. In particular, recent advances on cell engineering for improving plasmid DNA production are discussed.

An erratum to this chapter is available at http://dx.doi.org/10.1007/978-1-61779-433-9_35

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-61779-433-9_35

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Acknowledgments

This work was supported by CONACyT grants 84447 and 101847, PROMEP 47410089, and PAPIIT-UNAM IN-223210.

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Authors and Affiliations

  1. Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Mexico City, Mexico

    Alvaro R. Lara

  2. Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico

    Octavio T. Ramírez

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  1. Alvaro R. Lara
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  2. Octavio T. Ramírez
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Correspondence to Alvaro R. Lara .

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  1. Dept. Chemistry & Physics, Arkansas State University, Jonesboro, University Loop E. 504, State University, 72401, Arkansas, USA

    Argelia Lorence

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Lara, A.R., Ramírez, O.T. (2012). Plasmid DNA Production for Therapeutic Applications. In: Lorence, A. (eds) Recombinant Gene Expression. Methods in Molecular Biology, vol 824. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-433-9_14

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