Abstract
Genome-wide functional annotation of genes is one of the major challenges in current biology. Such investigation requires a high-throughput methodology for efficient and parallel overexpression or silencing of multiplexed genes in living cells. The transfection method described here employs an electric pulse and a cell-chip technology, and provides the possibility of analyzing gene functions in a high-throughput manner.
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Acknowledgments
The part of this work was supported by Grants-in-Aid for Scientific Research (No. 15310090), Ministry of Education, Culture, Sports, Science and Technology (MEXT) and Kobe Cluster, the Knowledge-Based Cluster Creation Project, MEXT.
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Kato, K., Iwata, H. (2011). High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation. In: Palmer, E. (eds) Cell-Based Microarrays. Methods in Molecular Biology, vol 706. Humana Press. https://doi.org/10.1007/978-1-61737-970-3_15
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DOI: https://doi.org/10.1007/978-1-61737-970-3_15
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