Cell-Based Microarrays

Methods and Protocols

  • Ella Palmer

Part of the Methods in Molecular Biology book series (MIMB, volume 706)

Table of contents

  1. Front Matter
    Pages i-x
  2. Ella Palmer
    Pages 1-12
  3. Holger Erfle, Tautvydas Lisauskas, Christoph Claas, Jürgen Reymann, Vytaute Starkuviene
    Pages 73-81
  4. Andreas Konrad, Ramona Jochmann, Elisabeth Kuhn, Elisabeth Naschberger, Priya Chudasama, Michael Stürzl
    Pages 107-118
  5. Satoshi Yamaguchi, Erika Matsunuma, Teruyuki Nagamune
    Pages 151-157
  6. Andrew L. Hook, Nicolas H. Voelcker, Helmut Thissen
    Pages 159-170
  7. Salvatore Pernagallo, Juan J. Diaz-Mochon
    Pages 171-180
  8. Ken-ichiro Kamei, Jing Sun, Hsian-Rong Tseng, Robert Damoiseaux
    Pages 191-206
  9. Back Matter
    Pages 207-209

About this book

Introduction

As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.

Keywords

Antibody arrays Functional analysis High throughput Live imaging Microfluidic image cytometry Reverse transfection Robotic printing Sub-cellular localization Transfection rates cDNAs and shRNAs

Editors and affiliations

  • Ella Palmer
    • 1
  1. 1.MRC Clinical Sciences Centre, Faculty of MedicineImperial College LondonLondonUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61737-970-3
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61737-969-7
  • Online ISBN 978-1-61737-970-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book