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Gene delivery to cells on a miniaturized multiwell plate for high-throughput gene function analysis

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Abstract

The transfectional microarray is a promising tool to conduct high-throughput analysis of gene function. In this study, a miniaturized multiwell plate was prepared by applying a silicone rubber sheet with holes on a gold electrode. The combination of electroporation and a miniaturized multiwell plate successfully achieved spatially controlled gene delivery with absence of cross-contamination between genes. Furthermore, we found that gene delivery efficiency was not dependent on conditions such as plasmid loading, electric field strength, and pulse duration.

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Authors and Affiliations

  1. Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan

    Ellyana Njatawidjaja & Hiroo Iwata

Authors
  1. Ellyana Njatawidjaja
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  2. Hiroo Iwata
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Correspondence to Hiroo Iwata.

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Njatawidjaja, E., Iwata, H. Gene delivery to cells on a miniaturized multiwell plate for high-throughput gene function analysis. Anal Bioanal Chem 392, 405–408 (2008). https://doi.org/10.1007/s00216-008-2281-8

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  • DOI: https://doi.org/10.1007/s00216-008-2281-8

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