Overview
- Editors:
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Shelley D. Minteer
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Department of Chemistry, St. Louis University, St. Louis, USA
- Provides protocols for enzyme stabilization that can be used for any enzyme of interest to the reader
- Includes up-to-date protocols on the latest enzyme immobilization techniques
- Provides a thorough review of methods for accurately determining enzyme activity and stability
- Procedures are scaleable from microscale molecular biology assays to industrial bioprocessing
- Includes supplementary material: sn.pub/extras
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Table of contents (17 protocols)
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- Michael J. Moehlenbrock, Shelley D. Minteer
Pages 1-7
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- Andreas Buthe, Songtao Wu, Ping Wang
Pages 37-48
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- Allan E. David, Arthur J. Yang, Nam Sun Wang
Pages 49-66
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- Keith J. Stine, Kenise Jefferson, Olga V. Shulga
Pages 67-83
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- Glenn R. Johnson, Heather R. Luckarift
Pages 85-97
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- Zorica D. Knežević-Jugović, Dejan I. Bezbradica, Dušan Ž. Mijin, Mirjana G. Antov
Pages 99-111
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- Sabina Besic, Shelley D. Minteer
Pages 113-131
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- Munishwar N. Gupta, Smita Raghava
Pages 133-145
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- Nenad B. Milosavić, Radivoje M. Prodanović
Pages 155-163
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- Alexey A. Vertegel, Vladimir Reukov, Victor Maximov
Pages 165-182
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- Patrick A. Johnson, Hee Joon Park, Ashley J. Driscoll
Pages 183-191
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- Amanda S. Harper-Leatherman, Jean Marie Wallace, Debra R. Rolison
Pages 193-205
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Back Matter
Pages 227-229
About this book
In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biologyâ„¢ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.
Editors and Affiliations
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Department of Chemistry, St. Louis University, St. Louis, USA
Shelley D. Minteer