Abstract
Advances in molecular biology and in the genetics of Arabidopsis thaliana have led to this organism becoming an important model for the analysis of meiosis in plants. Cytogenetic investigations are pivotal to meiotic studies and a number of technological improvements for Arabidopsis cytology have provided a range of tools to investigate chromosome behaviour during meiosis. This chapter includes protocols on basic cytology, FISH analysis, immunocytology, a procedure for a meiotic time course and electron microscopy.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Jones, G.H., and Franklin, F.C.H. (2007) Meiosis in Arabidopsis thaliana: Recombination, Chromosome Organization and Meiotic Progression, in Genome Dynamics and Stability (Lankenau D.H., ed) Springer, Berlin, Vol 1, pp 1861–2273.
Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408, 796–815.
Armstrong, S.J., and Jones, G.H. (2003) Meiotic cytology and chromosome behaviour in wild-type Arabidopsis thaliana. J. Exp. Botany 54, 1–10.
Jones, G.H., Armstrong, S.J., Caryl, A.P., and Franklin, F.C.H. (2003) Meiotic chromosome synapsis and recombination in Arabidopsis thaliana; an integration of cytological and molecular approaches. Chromosome Res. 11, 205–215.
Ross, K.J., Fransz, P., and Jones, G.H. (1996) A light microscopic atlas of meiosis in Arabidopsis thaliana. Chromosome Res. 4, 507–516.
Fransz, P., Armstrong, S.J., Alonso-Blanco, C., Fischer, T.C., Torrez-Ruiz, R.A., and Jones, G.H. (1998) Cytogenetics for the model system Arabidopsis thaliana. Plant J. 13, 867–876.
Sanchez-Moran, E., Armstrong, S.J., Santos, J.L., Franklin, F.C.H., and Jones G.H. (2001) Chiasma formation in Arabidopsis thaliana accession Wassileskija and in two meiotic mutants. Chromosome Res. 9, 121–128.
Armstrong, S.J., Franklin, F.C.H., and Jones, G.H. (2003). A meiotic time course for Arabidopsis thaliana. Sex. Plant Reprod. 16, 141–149.
Higgins, J.D., Armstrong, S.J., Franklin, F.C.H., and Jones, G.H. (2004). The Arabidopsis MutS homologue At MSH4 functions at an early step in recombination; evidence for two classes of recombination in Arabidopsis. Genes Dev. 18, 2557–2570.
Pradillo, M., Lopez, E., Romero, C., Sanchez-Moran, E., Cunado, N., and Santos, J.L.. (2007). An analysis of univalent segregation in meiotic mutants of Arabidopsis thaliana: a possible role for the synaptonemal complex. Genetics 178, 505–511.
Higgins, J.D., Sanchez-Moran, E., Armstrong, S.J., Jones, G.H., and Franklin, F.C.H.. (2005). The Arabidopsis synaptonemal complex protein ZYP1 is required for chromosome synapsis and normal fidelity of crossing over. Genes De. 19, 2488–2500.
Acknowledgements
The work in our laboratories is supported by the Royal Society and the Biotechnology and Biological Sciences Research Council (BBSRC). Technical support has been provided by Steve Price.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Armstrong, S.J., Sanchez-Moran, E., Chris, F., Franklin, H. (2009). Cytological Analysis of Arabidopsis thaliana Meiotic Chromosomes. In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 558. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-103-5_9
Download citation
DOI: https://doi.org/10.1007/978-1-60761-103-5_9
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-102-8
Online ISBN: 978-1-60761-103-5
eBook Packages: Springer Protocols