Microcoil NMR spectroscopy is based on the increase of coil sensitivity for smaller coil diameters (approximately 1/d). Microcoil NMR probes deliver a remarkable mass-based sensitivity increase (8- to 12-fold) when compared with commonly used 5-mm NMR probes. Although microcoil NMR probes are a well established analytical tool for small molecule liquid-state NMR spectroscopy, after spectroscopy only recently have microcoil NMR probes become available for biomolecular NMR spectroscopy. This chapter highlights differences between commercially available microcoil NMR probes suitable for biomolecular NMR spectroscopy. Furthermore, it provides practical guidance for the use of microcoil probes and shows direct applications for structural biology and structural genomics, such as optimal target screening and structure determination, among others.
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Acknowledgments
The authors thank Mark O'Neil-Johnson (Sequoia Sciences) for support and encouragement for the microcoil measurements and Timothy Peck (M. R. M.) for discussions. W. P. thanks his laboratory members, especially Tingting Ju, Matthew Kelker, and Barbara Dancheck, for discussions and critical reading of the manuscript. W. P. is the Manning Assistant Professor for Medical Science at Brown University.
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Hopson, R.E., Peti, W. (2008). Microcoil NMR Spectroscopy: a Novel Tool for Biological High Throughput NMR Spectroscopy. In: Kobe, B., Guss, M., Huber, T. (eds) Structural Proteomics. Methods in Molecular Biology™, vol 426. Humana Press. https://doi.org/10.1007/978-1-60327-058-8_30
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