Abstract
By definition, structural genomics centers must be able to address a large number of diverse protein targets. The methods developed should permit parallel and cost-effective processing while allowing for the diverse nature of proteins. Our approach to this problem is a multi-tiered effort where targets are characterized and categorized by behavior and processed in parallel by appropriate methods. The Joint Center for Structural Genomics (JCSG) has applied this tactic to create a fully integrated and scaleable structure determination pipeline. Highlights of the development of the current pipeline for protein production and crystallization are presented here.
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References
S.A. Lesley P. Kuhn A. Godzik A.M. Deacon I. Mathews A. Kreusch G. Spraggon H.E. Klock D. McMullan T. Shin J. Vincent A. Robb L.S. Brinen M.D. Miller T.M. McPhillips M.A. Miller D. Scheibe J.M. Canaves C. Guda L. Jaroszewski T.L. Selby M.A. Elsliger J. Wooley S.S. Taylor K.O. Hodgson I.A. Wilson P.G. Schultz R.C. Stevens (2002) Proc. Natl. Acad. Sci. U.S.A. 99 11664–11669 Occurrence Handle10.1073/pnas.142413399 Occurrence Handle12193646
M. DiDonato A.M. Deacon H.E. Klock D. McMullan S.A. Lesley (2004) J. Struct. Funct. Genom. 5 133–146 Occurrence Handle10.1023/B:JSFG.0000029194.04443.50
S.A. Lesley (2001) Protein Expr. Purif. 22 159–164 Occurrence Handle10.1006/prep.2001.1465 Occurrence Handle11437590
R.C. Stevens (2000) Curr. Opin. Struct. Biol. 10 558–563 Occurrence Handle10.1016/S0959-440X(00)00131-7 Occurrence Handle11042454
Abola, E., Kuhn, P., Earnest, T. and Stevens, R.C. (2000) Nat. Struct. Biol Suppl, 973–977
R.L. Strausberg E.A. Feingold R.D. Klausner F.S. Collins (1999) Science 286 455–457 Occurrence Handle10.1126/science.286.5439.455 Occurrence Handle10521335
InstitutionalAuthorNameMGC (Mammalian Gene Collection) Program Team (2002) Proc. Natl. Acad. Sci. U.S.A. 99 16899–16903
Q. Liu M.Z. Li D. Leibham D. Cortez S.J. Elledge (1999) Curr. Biol. 8 1300–1309 Occurrence Handle10.1016/S0960-9822(07)00560-X
J.L. Hatley G.F. Temple M.A. Brasch (2000) Genome Res. 10 1788–1795 Occurrence Handle10.1101/gr.143000 Occurrence Handle11076863
S. Johnston J.H. Lee D.S. Ray (1985) Gene 34 137–45 Occurrence Handle10.1016/0378-1119(85)90121-0 Occurrence Handle4007491
R. Page S.K. Grzechnik A. Kreusch G. Spraggon R.C. Stevens S.A. Lesley (2003) Acta Cryst. D. 59 1028–1037 Occurrence Handle10.1107/S0907444903007790
J.C. Wootton S. Federhen (1993) Comput. Chem. 17 149–163 Occurrence Handle10.1016/0097-8485(93)85006-X
R. Linding R.B. Russell V. Neduva T.J. Gibson (2003) Nucleic Acids Res. 31 3701–3708 Occurrence Handle10.1093/nar/gkg519 Occurrence Handle12824398
D. Pantazatos J.S. Kim H.E. Klock R.C. Stevens I.A. Wilson S.A. Lesley V.L. Woods (2004) Proc. Natl. Acad. Sci. U.S.A. 101 751–756 Occurrence Handle10.1073/pnas.0307204101 Occurrence Handle14715906
G. Spraggan D. Pantazatos H.E. Klock I.A. Wilson V.L. Woods SuffixJr. S.A. Lesley (2004) Protein Sci. 13 3187–3199 Occurrence Handle10.1110/ps.04939904 Occurrence Handle15557262
C.S. Goh N. Lan S.M. Douglas B. Wu N. Echols A. Smith D. Milburn G.T. Montelione H. Zhao M. Gerstein (2004) J. Mol. Biol. 336 115–130 Occurrence Handle10.1016/j.jmb.2003.11.053 Occurrence Handle14741208
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Lesley, S.A., Wilson, I.A. Protein Production and Crystallization at the Joint Center for Structural Genomics. J Struct Funct Genomics 6, 71–79 (2005). https://doi.org/10.1007/s10969-005-2897-2
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DOI: https://doi.org/10.1007/s10969-005-2897-2