Editors:
First book of protocols to cover techniques in a new and emerging field
Offers step by step methods and protocols for reproducible lab experiments
Each protocol has a Notes section where the major researchers in the field offer tricks and hints of the trade
Part of the book series: Methods in Molecular Biology (MIMB, volume 426)
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Table of contents (39 protocols)
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Front Matter
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Protein Target Selection, Bioinformatic Approaches, and Data Management
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Protein Production
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Biophysical and Functional Characterization of Proteins
About this book
Structural genomics is a newly emerging field that has arisen following the successful footsteps of the major sequencing efforts generally bundled under the heading "genomics". In Structural Proteomics: High Throughput Methods, readers are provided with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimized. This volume includes chapters describing in-depth the individual steps in the Structural Genomics pipeline, as well as overviews of individual Structural Genomics initiatives. Topics include Target Selection for structural genomics, Protein structure modeling, High-throughput cloning, Mass Spectrometry modeling. Structural Proteomics: High Throughput Methods, encourages its readers to access further details on the methodologies in on-line resources as well as in cited literature making it an invaluable resource. Most methods in this important book are as amenable to small laboratories as to large consortia, and do not require major investments in facilities. This essential volume provides insight into the diversity of approaches adopted by different laboratories and proves that 'high throughput' is really the defining characteristic of structural genomics.
Reviews
From the reviews:
"This book compiles an impressive array of contributions from a variety of contributors … usually in a format that is accessible to any jobbing structural biologist, or those other molecular biologists with an linking to press their molecular targets into crystal screens or NMR tubes. … In sum, this is a book it is useful to have to hand … ." (Paul C. Driscoll, ChemBioChem, Issue 9, August, 2008)
"This book ... details some of the `tricks of the trade' involved in the field of structural genomics. ... This will be a useful text for laboratories already involved in fields covered in the book who want to increase the number of projects in the lab or for those who want to expand into neighbouring fields." (Darren Thompson, Microbiology Today, November, 2008)
Editors and Affiliations
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School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Queensland, Australia
Bostjan Kobe
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School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
Mitchell Guss
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School of Molecular and Microbial Sciences and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia
Thomas Huber
Bibliographic Information
Book Title: Structural Proteomics
Book Subtitle: High-Throughput Methods
Editors: Bostjan Kobe, Mitchell Guss, Thomas Huber
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-058-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-809-6Published: 03 March 2008
Softcover ISBN: 978-1-61737-789-1Published: 10 December 2010
eBook ISBN: 978-1-60327-058-8Published: 10 June 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XXVI, 602
Number of Illustrations: 120 b/w illustrations, 10 illustrations in colour
Topics: Protein Science, Proteomics, Microbial Genetics and Genomics, Bioinformatics, Cell Biology