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Proteomic Profiling of Integrin Adhesion Complex Assembly

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Protein Complex Assembly

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1764))

Abstract

Cell adhesion to components of the cellular microenvironment via cell-surface adhesion receptors controls many aspects of cell behavior in a range of physiological and pathological processes. Multimolecular complexes of scaffolding and signaling proteins are recruited to the intracellular domains of adhesion receptors such as integrins, and these adhesion complexes tether the cytoskeleton to the plasma membrane and compartmentalize cellular signaling events. Integrin adhesion complexes are highly dynamic, and their assembly is tightly regulated. Comprehensive, unbiased, quantitative analyses of the composition of different adhesion complexes over the course of their formation will enable better understanding of how the dynamics of adhesion protein recruitment influence the functions of adhesion complexes in fundamental cellular processes. Here, a pipeline is detailed integrating biochemical isolation of integrin adhesion complexes during a time course, quantitative proteomic analysis of isolated adhesion complexes, and computational analysis of temporal proteomic data. This approach enables the characterization of adhesion complex composition and dynamics during complex assembly.

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Acknowledgments

J.A. Askari, J.D. Humphries, M.J. Humphries, and other members of the Humphries Laboratory (University of Manchester) are gratefully acknowledged for the development and optimization of the integrin adhesion complex purification protocol described herein, which was funded by the Wellcome Trust. A.B. is funded by Cancer Research UK (grant C157/A15703 to M.C. Frame, University of Edinburgh).

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  1. Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK

    Adam Byron

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  1. Adam Byron
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Correspondence to Adam Byron .

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  1. MRC Human Genetics Unit, Institute of Genetics & Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom

    Joseph A. Marsh

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Byron, A. (2018). Proteomic Profiling of Integrin Adhesion Complex Assembly. In: Marsh, J. (eds) Protein Complex Assembly. Methods in Molecular Biology, vol 1764. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7759-8_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7759-8_13

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