Abstract
Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.
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Acknowledgement
This work was supported by the German Research Foundation DFG.
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Zhu, G., Walter, JG. (2015). Aptamer-Modified Magnetic Beads in Affinity Separation of Proteins. In: Reichelt, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 1286. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2447-9_7
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DOI: https://doi.org/10.1007/978-1-4939-2447-9_7
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2446-2
Online ISBN: 978-1-4939-2447-9
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