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  • © 2015

Affinity Chromatography

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols

  • Provides step-by-step details essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1286)

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Softcover Book USD 119.99
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Hardcover Book USD 109.99
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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
  2. Affinity Chromatography: A Historical Perspective

    • David S. Hage, Ryan Matsuda
    Pages 1-19
  3. General Modes and Ligands of Affinity Chromatography

    1. Front Matter

      Pages 21-21
    2. Protein Purification by Aminosquarylium Cyanine Dye-Affinity Chromatography

      • Vânia C. Graça, Fani Sousa, Paulo F. Santos, Paulo S. Almeida
      Pages 23-33
    3. One-Step Purification of Phosphinothricin Acetyltransferase Using Reactive Dye-Affinity Chromatography

      • Cunxi Wang, Thomas C. Lee, Kathleen S. Crowley, Erin Bell
      Pages 35-42
    4. Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes

      • Handan Yavuz, Nilay Bereli, Fatma Yılmaz, Canan Armutcu, Adil Denizli
      Pages 43-46
    5. Cell Affinity Separations on Microfluidic Devices

      • Yan Gao, Wenjie Li, Ye Zhang, Dimitri Pappas
      Pages 55-65
    6. Aptamer-Modified Magnetic Beads in Affinity Separation of Proteins

      • Guohong Zhu, Johanna-Gabriela Walter
      Pages 67-82
    7. Robotic High-Throughput Purification of Affinity-Tagged Recombinant Proteins

      • Simone C. Wiesler, Robert O. J. Weinzierl
      Pages 97-106
  4. Macroporous Polymers as Matrix in Affinity Chromatography

    1. Front Matter

      Pages 171-171
    2. Introduction to Macroporous Cryogels

      • Senta Reichelt
      Pages 173-181
    3. Cryogels with Affinity Ligands as Tools in Protein Purification

      • Solmaz Hajizadeh, Bo Mattiasson
      Pages 183-200
    4. Direct Capture of His6-Tagged Proteins Using Megaporous Cryogels Developed for Metal-Ion Affinity Chromatography

      • Naveen Kumar Singh, Roy N. DSouza, Noor Shad Bibi, Marcelo Fernández-Lahore
      Pages 201-212

About this book

The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.

Editors and Affiliations

  • Leibniz-Institut für Oberflächenmodifizierung, Leipzig, Germany

    Senta Reichelt

Bibliographic Information

  • Book Title: Affinity Chromatography

  • Book Subtitle: Methods and Protocols

  • Editors: Senta Reichelt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2447-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2446-2Published: 07 March 2015

  • Softcover ISBN: 978-1-4939-5584-8Published: 22 October 2016

  • eBook ISBN: 978-1-4939-2447-9Published: 06 March 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIII, 341

  • Number of Illustrations: 41 b/w illustrations, 37 illustrations in colour

  • Topics: Biochemistry, general, Chromatography

Buy it now

Buying options

Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access