Abstract
Our comparative immunohistochemical and immunocytochemical investigations on dibranchiate and tetrabranchiate cephalopods showed that haemocyanin synthesis is localized in a special type of cell which is cytomorphologically similar in both genera. In Sepia these haemocyanin-producing cells are located in the branchial gland (polygonal cells) and also in the midgut gland (triangular replacement cells), especially in newly hatched animals. In Nautilus these cells can be identified in the terminal alveoli of the midgut gland, where they are described as basal cells. Some haemocyanin synthesizing cells are scattered in the tissue of the ligamentum branchiale in the gill complex.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Albrecht, U., and Markl, J., 1995, Pore cells as a possible site of haemocyanin synthesis in the keyhole limpet Megathura crenulata, Verh. Dtsch. Zool. Ges, 88.1: 156.
Beintema, J. J., 1990, Evolutionary aspects of dioxygen carriers, in: Invertebrate Dioxygen Carriers (Préaux, G. and Lontie, R., eds.), Leuven: Leuven University Press, pp. 513–520.
Boletzky, S.v., 1967, Die embryonale Ausgestaltung der frühen Mitteldarmanlage von Octopus vulgaris Lam., Rev. Suisse Zool, 74: 555–562.
Bonaventura, C, Bonaventura, J., Miller, K. I., and van Holde, K. E., 1981, Hemocyanin of the chambered Nautilus:structure-function relationships, Arch. Biochem. Biophys, 211(2):589–598
Bradford, M. M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem, 72: 248–254.
Decleir, W., Lemaire, J., and Richard, A., 1971, The differentiation of blood proteins during ontogeny in Sepia of-ficinalis L., Comp. Biochem. Physiol, 40B:923–930.
Dilly, P. N., and Messenger, J. B., 1972, The branchial gland: a site of hemocyanin synthesis in Octopus, Z. Zeilforsch, 132:193–201.
Fahrenbach, W.H., 1970, The cyanoblast. Haemocyanin formation in Limulus polyphemus, J. Cell Biol, 44: 457–491.
Friemel, H., 1984, Immunologische Arbeitsmethoden. Fischer Verlag, Stuttgart.
Ghiretti-Magaldi, A., Milanesi, C, and Salvato, B., 1973, Identification of Hemocyanin in the cyanocytes of Car-cinus maenas, Experientia 29: 1265–1267.
Hennecke, R., Gellissen, G., Spindler-Barth, M., and Spindler, K. D., 1990, Haemocyanin synthesis in the crayfish Astracus leptodactylus, in: Invertebrate Dioxygen Carriers (Préaux, G. and Lontie, R., eds.), Leuven: Leuven University Press, pp. 503–506.
Hennig, W., 1995, Phylogenetic Systematics, Univ. Illinois Press, Urbana.
Humphrey, C. D., and Pittman, F. E., 1974, A simple methylene blue azure II basic fuchsin stain for epoxy embedded tissue sections, Stain Technology 49: 9–14.
Laemmli, U. K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227:680–685.
Linné, K., 1758, Systema Naturae 10, Stockholm.
Markl, J., Stumpp, S., Bosch, F. X. Bosch, and Voit, R., 1990, Hemocyanin biosynthesis in the tarantula Eurypelma cali-fornicum, studied by in situ hybridization and immunoelectron microscopy, in: Invertebrate Dioxygen Carriers (Préaux, G. and Lontie, R., eds.), Leuven: Leuven University Press, pp. 497–50
McKinney, M. M., and Parkinson, A., 1987, A simple, non-chromatographic procedure to purify immunglobulins from serum and ascites fluid, Journal of Immunological Methods, 96:271–278.
Meister, G., 1972, Organogenese von Loligo vulgaris LAM. (Mullusca, Cephalopoda, Teuthoidea, Myopsida, Loliginidae), Zool. Jb. Anat, 89: 247–300.
Messenger, J. B., Muzii, E. O., Nardi, G., and Steinberg, H., 1974, Haemocyanin synthesis and the branchial gland of Octopus, Nature 250:154–155.
Muzii, E., 1981, Intracellular polymerized haemocyanin in the branchial gland of a cephalopod, Cell Tissue Res, 220:435–438.
Naef, A., 1928, Die Cephalopoden. Fauna und Flora des Golfes von Neapel 35 Monographie 1. Teil, 2. Bd.
Ouchterlony, Ö., and Nilsson, L. A., 1978, Immunodiffusion and immunoelectrophoresis, in: Handbook of Experimental Immunology (D.M. Weir, ed.), Blackwell Scientific Publications, Oxford.
Préaux, G., Vandamme, A., Béthune, B., Jacobs, M. P., and Lontie, R., 1986, in: Invertebrate Dioxygen Carriers (B. Linzen, ed.), Springer Verlag, Berlin und Heidelberg, pp. 485–488.
Rainer, J., and Brouwer, M., 1993, Hemocyaninsynthesis in the blue crab Callinectes sapidus, Comp. Biochem. Physiol, 104b(1):69–73.
Reger, J. F., 1973, A fine structure study on haemocyanin formation in the slug Umax sp., J. Ultrastructure Res, 43: 377–387.
Reynolds, E. S., 1963, The use of leadcitrate of high pH as an electron-opaque stain in electron microscopy, J. Cell. Biol, 17:208–217.
Ruth, P., Blum, W., and Bille, J., 1996, Immunocytochemical reaction of a haemocyanin antibody in the midgut gland of Nautilus (Cephalopoda, Tetrabranchiata), Experientia 52: 549–553.
Ruth, P., Schipp, R., and Klüssendorf, B., 1988, Cytomorphology and copper content of the basal cells in The midgut gland of Nautilus (Cephalopoda, Tetrabranchiata). A contribution to the localization of hemocyanin synthesis, Zoomorphology 108:1–11.
Saure, H., Schipp, R., and Magnier, Y., 1987, Morphology and functional aspects of the branchial complex in Nautilus (Tetrabranchiata, Cephalopoda), Zool. Jb. Anat, 116:39–62.
Schipp, R., Höhn, P., and Ginkel, G., 1973, Elektronenmikroskopische und histochemische Untersuchungen zur Funktion der Branchialdrüse (Parabranchialdrüse) der Cephalopoda, Z Zellforsch, 139:253–269.
Schmekel, L., and Weischer, M. L., 1973, Die Blutdrüse der Doridoidea (Gastropoda, Opistobranchia) als Ort möglicher Hämocyanin Synthese, Z. Morph. Tiere 76: 261–284.
Schönenberger, N., Cox, J. A., and Gabbiani, G., 1980, Evidence for hemocyanin formation in the compound eye of Squilla mantis (Crustacea, Stomatopoda), Cell. Tissue Res, 205:397–409.
Senkbeil, E. G., und Wriston, J. C, 1981, Hemocyanin synthesis in the American lobster, Homarus americanus, Comp. Biochem. Physiol, 68B: 163–171.
Skelding, J. M., and Newell, R F., 1975, On the function of the pore cells in the connective tissue of the terrestrial pulmonate mollusks, Cell Tissue Res, 156:381–390.
Sminia, T., 1972, Structure and function of blood and connective tissue cells of the fresh water pulmonate Lym-naea stagnalis studied by electron microscopy and enzyme histochemistry, Z. Zellforsch, 130:497–526.
Sminia, T., 1974, Haemotopoiesis in the freshwater snail Lymnaea stagnalis studied by electron microscopy and autoradiography, Cell Tissue Res, 150:443–454.
Sminia, T., and Boer, H. H., 1973, Haemocyanin production in pore cells of the freshwater snail Lymnaea stagnalis, Z. Zellforsch., 145: 443–445.
Sminia, T., and Vlugt-van Daalen, J. E., 1977, Haemocyanin synthesis in pore cells of the terrestrial snail Helix as-persa, Cell Tiss. Res, 183: 299–301.
Taki, I., 1964, On the morphology and physiology of the branchial gland in cephalopoda, J. Fac. Fish. Anim. Husb, 5:345–417.
Tanabe, K., Tsukahara, J., Fukuda, Y., and Taya, Y, 1991, Histology of a living Nautilus embryo: preliminary observations, Journal of cephalopod biology, 2: 13–22.
van Leeuwen, F., 1986, Pitfalls in immunocytochemistry with special reference to the specificity problems in the localization of neuropeptides, Am. J. Anat, 175:363–377.
Vardell, I. M., Tapia, F. J., Probert, L., Buchan, A. M. J., Gu, J., DeMey, J., Bloom, S. R., and Polak, J. M., 1982, Peptides, 149-150, cited from Noorda, S. and Polak, J. M. Immunochemistry of regulatory peptides. In: Techniques in immunocytochemistry (G.R. Bullock and P. Petrusz, eds.), Academic Press, London, v. 3, pp. 16–154.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Ruth, P., Schimmelpfennig, R., Schipp, R. (1999). Comparative Immunohistochemical and Immunocytochemical Investigations on the Location of Haemocyanin Synthesis in Dibranchiate and Tetrabranchiate Cephalopods (Sepia and Nautilus). In: Olóriz, F., Rodríguez-Tovar, F.J. (eds) Advancing Research on Living and Fossil Cephalopods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4837-9_14
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4837-9_14
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7193-9
Online ISBN: 978-1-4615-4837-9
eBook Packages: Springer Book Archive