Abstract
The artificial bilayer single-channel recording technique is commonly used to observe detailed pharmacological properties of various ion channel proteins. It permits easy control of the solution and membrane lipid composition, and is also compatible with pharmacological screening devices. However, its use is limited due to low measurement efficiency. Here, we develop a novel artificial bilayer single-channel recording technique in which bilayers are made and channels are reconstituted into the membranes by contacting a gold electrode to the lipid-solution interface. Using this technique, we measured the single-channel currents of two channel-forming peptides, gramicidin and alamethicin, and a channelforming protein, α-hemolysin. This technique requires only one action, allowing the technique to potentially be combined with high-throughput screening devices.
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Acknowledgments
The authors thank Dr. Peter Karagiannis for carefully revising the manuscript. This work was supported by grants from the Research Foundation for Opto-Science and Technology, the NOVARTIS Foundation (Japan) for the Promotion of Science, the Japan Society for the Promotion of Science (JSPS) (KAKENHI Grant Numbers 22370059, 24657110, 15K13725), the Foundation Advanced Technology Institute (ATI), and the Science Research Promotion Fund of the Promotion and Mutual Aid Corporation for Private Schools of Japan.
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Okuno, D., Hirano, M., Yokota, H. et al. A Simple Method for Ion Channel Recordings Using Fine Gold Electrode. ANAL. SCI. 32, 1353–1357 (2016). https://doi.org/10.2116/analsci.32.1353
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DOI: https://doi.org/10.2116/analsci.32.1353