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Recording of Ion Channel Activity in Planar Lipid Bilayer Experiments

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Ion Channels

Part of the book series: Methods in Molecular Biology ((MIMB,volume 998))

Abstract

Planar lipid bilayer is an electrophysiological technique that enables study of functional activities of ion channels, porins, and other pore-forming molecular complexes. The main purpose of this method is to monitor ion channels’ behavior at the single molecule level in the artificial membranes. Here, I describe the details of this technique that will underline formation of the lipid bilayers and incorporation and activation of the ion channel protein.

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References

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Zakharian, E. (2013). Recording of Ion Channel Activity in Planar Lipid Bilayer Experiments. In: Gamper, N. (eds) Ion Channels. Methods in Molecular Biology, vol 998. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-351-0_8

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  • DOI: https://doi.org/10.1007/978-1-62703-351-0_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-350-3

  • Online ISBN: 978-1-62703-351-0

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