Skip to main content
SpringerLink
Log in

Quantitative Measurement of 17β-Estradiol and Estriol in River Water by Time-Resolved Fluoroimmunoassay

  • Original Paper
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

A sensitive method for detecting 17β-estradiol (E2) and estriol (E3) in river water has been developed, based on the time-resolved fluoroimmunoassay by using a fluorescent europium chelate label, 4,4′-bis(1″,1″,1″,2″,2″,3″,3″-heptafluoro-4″,6″-hexanedion-6″-yl)-chlorosulfo-o-terphenyl (BHHCT)-Eu3+. In the E2 assay, microtiter plates were coated with the E2-bovine serum albumin (BSA) conjugate. The anti-17β-estradiol antibody, the biotinylated goat anti-rabbit IgG antibody and the BHHCT-Eu3+ labeled streptavidin (SA)-BSA conjugate were used. In the E3 assay, the goat anti-rabbit IgG antibody was coated on a microtiter plate. The anti-estriol antibody and the BHHCT-Eu3+ labeled E3-BSA conjugate were used. The detection limits for E2 and E3 were 2.3 pg/ml and 4.3 pg/ml, respectively, and the analytical recoveries were 95–120%. Quantitative measurement of estrogens in river water was carried out for Kanda River (Tokyo, Japan) by using the method. The E2 and E3 levels were 32 pg/ml and 5.5 pg/ml, respectively. The detection limits of the present method are in the same orders of magnitude as those of ELISA for E2, and are 1 -2 orders of magnitude better for E3.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C. Desbrow, E. J. Routledge, G. C. Brighty, J. P. Sumpter, and M. Waldock, Environ. Sci. Technol., 1998, 32, 1549.

    Article  CAS  Google Scholar 

  2. E. J. Routledge, D. Sheahan, C. Desbrow, G. C. Brighty, M. Waldock, and J. P. Sumpter, Environ. Sci. Technol., 1998, 32, 1559.

    Article  CAS  Google Scholar 

  3. G. H. Panter, R. S. Thompson, and J. P. Sumpter, Aquatic Toxicol., 1998, 42, 243.

    Article  CAS  Google Scholar 

  4. G. H. Panter, R. S. Thompson, and J. P. Sumpter, Environ. Sci. Technol., 2000, 34, 2756.

    Article  CAS  Google Scholar 

  5. T. P. Rodgers-Gray, S. Jobling, C. Kelly, S. Morris, G. Brighty, M. J. Waldock, J. P. Sumpter, and C. R. Tyler, Environ. Sci. Technol., 2001, 35, 462.

    Article  CAS  Google Scholar 

  6. H.-B. Lee and T. E. Peart, J. AOAC, 1998, 81, 1209.

    Article  CAS  Google Scholar 

  7. C. Baronti, R. Curini, G. D’ascenzo, A. Di Corcia, A. Gentili, and R. Samperi, Environ. Sci. Technol., 2000, 34, 5059.

    Article  CAS  Google Scholar 

  8. G. W. Aherne and R. Briggs, J. Pharm. Pharmacol., 1989, 41, 735.

    Article  CAS  Google Scholar 

  9. L. S. Shore, M. Gurevitz, and M. Shemesh, Bull. Environ. Contam. Toxicol., 1993, 51, 361.

    Article  CAS  Google Scholar 

  10. S. A. Snyder, T. L. Keith, D. A. Verbrugge, E. M. Snyder, T. S. Gross, K. Kannan, and J. P. Giesy, Environ. Sci. Technol., 1999, 33, 2814.

    Article  CAS  Google Scholar 

  11. H. Tajima, K. Tsujimura, and M. Yamaguchi, Bunseki Kagaku, 2000, 49, 843.

    Article  CAS  Google Scholar 

  12. T. A. Ternes, M. Stumpf, J. Mueller, K. Haberer, R.-D. Wilken, and M. Servos, Scl. Total Environ., 1999, 225, 81.

    Article  CAS  Google Scholar 

  13. C. Kelly, J. Chromatogr. A, 2000, 872, 309.

    Article  CAS  Google Scholar 

  14. S. Nakamura, M. Takino, and S. Daishima, Bunseki Kagaku, 2000, 49, 181.

    Article  CAS  Google Scholar 

  15. M. J. López de Alda and D. Barceló, J. Chromatogr. A, 2000, 892, 391.

    Article  Google Scholar 

  16. Y. Ishii, S. Okita, M. Torigai, and S.-J. Yun, Bunseki Kagaku, 2000, 49, 753.

    Article  CAS  Google Scholar 

  17. J. Yuan, G. Wang, H. Kimura, and K. Matsumoto, Anal. Biochem., 1997, 254, 283.

    Article  CAS  Google Scholar 

  18. H. Kimura, J. Yuan, G. Wang, K. Matsumoto, and M. Mukaida, J. Anal. Toxicol., 1999, 23, 11.

    Article  CAS  Google Scholar 

  19. J. Yuan, G. Wang, H. Kimura, and K. Matsumoto, Anal. Sci., 1999, 15, 125.

    Article  CAS  Google Scholar 

  20. K. Yoshikawa, J. Yuan, K. Matsumoto, and H. Kimura, Anal. Sci., 1999, 15, 121.

    Article  CAS  Google Scholar 

  21. J. Yuan, K. Matsumoto, and H. Kimura, Anal. Chem., 1998, 70, 596.

    Article  CAS  Google Scholar 

  22. K. Matsumoto, J. Yuan, G. Wang, and H. Kimura, Anal. Biochem., 1999, 276, 81.

    Article  CAS  Google Scholar 

  23. “Provisional manual of the analysis on the exogenous endocrine disrupting chemicals”, 1998, Water Control Division, Water Quality Bureau, Environment Agency, Tokyo.

  24. R. A. Evangelista, A. Pollak, B. Allore, E. F. Templeton, R. C. Morton, and E. P. Diamandis, Clin. Biochem., 1988, 21, 173.

    Article  CAS  Google Scholar 

  25. S. Masunaga, T. Itazawa, T. Furuichi, Sunardi ], D. L. Villeneuve, K. Kannan, J. P. Giesy, and J. Nakanishi, Environ. Sci.}, 2000, 7

    CAS  Google Scholar 

  26. “Results of the survey on the endocrine disrupting chemicals in water environment (FY 1998)”, 1999, River Environmental Management Foundation, Ministry of Construction, Tokyo.

  27. “Results of the survey on the endocrine disrupting chemicals in water environment”, 2000, Bureau of Sewage Works, Tokyo Metropolitan Government, Tokyo.

  28. T. P. Rodgers-Gray, S. Jobling, S. Morris, C. Kelly, S. Kirby, A. Janbakhsh, J. E. Harries, M. J. Waldock, J. P. Sumpter, and C. R. Tyler, Environ. Sci. Technol., 2000, 34, 1521.

    Article  CAS  Google Scholar 

  29. T. A. Ternes, P. Kreckel, and J. Mueller, Sci. Total Environ., 1999, 225, 91.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Waseda University, Advanced Research Institute for Science and Engineering, Shinjuku, Tokyo, 169-8555, Japan

    Keisuke Majima, Takashi Fukui & Kazuko Matsumoto

  2. Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116012, China

    Jingli Yuan & Guilan Wang

Authors
  1. Keisuke Majima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takashi Fukui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jingli Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guilan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kazuko Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kazuko Matsumoto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Majima, K., Fukui, T., Yuan, J. et al. Quantitative Measurement of 17β-Estradiol and Estriol in River Water by Time-Resolved Fluoroimmunoassay. ANAL. SCI. 18, 869–874 (2002). https://doi.org/10.2116/analsci.18.869

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.18.869

Search

Navigation