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Rapid and sensitive determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone by biotin–streptavidin-amplified time-resolved fluoroimmunoassay

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  • Aquaculture
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Abstract

A competitive time-resolved fluoroimmunoassay (TRFIA) using a biotin–streptavidin system was developed for the detection and quantification of the furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in aquatic tissues. AMOZ-bovine serum albumin was coated to a solid phase and then competed with a free nitrophenyl derivative of AMOZ (2-NP-AMOZ) in standards or samples for binding with biotin-anti-AMOZ-ovalbumin polyantibody. Later, the complex was recognized by europium-labelled streptavidin, which was used as a novel tracer to amplify the signal, and it also reacted faster than europium-labelled second antibody in traditional TRFIA. After optimizing the reaction conditions, the method showed high sensitivity and specification to 2-NP-AMOZ with a half maximal inhibitory concentration of 0.190 μg/l and a sensitivity of 0.019 μg/l with a working range from 0.025 to 10 μg/l. The data from fish and shrimp samples indicated that the limit of detection was 0.021 μg/kg, and the recovery rates were 84.1–107.0% and 80.9–98.4%, respectively, with an average RSD below 10%. High correlation rates were observed in TRFIA, high performance liquid chromatography and universal enzyme-linked immunosorbent assay methods. The experiment above confirms that biotin–streptavidin-amplified TRFIA could be an ultrasensitive and accurate tool for screening large numbers of aquatic products for the determination of AMOZ residues.

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Acknowledgements

This work was supported by a Major Project of National Agriculture Product in Quality and Safety Risk Assessment (GJFP201700902).

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Author notes

  1. Yi Zhang and Chunhui Zhao contributed equally to this work, and should be considered as first authors.

Authors and Affiliations

  1. Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, 214063, China

    Yi Zhang, Bing Wu, Jue Zhang, Bin Zhou, Jun Fan & Biao Huang

  2. Beijing Aquatic Product Technology Promotion Department, Beijing, 100021, China

    Chunhui Zhao, Ying Li & Fang Lv

  3. School of Life Science, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China

    Biao Huang

Authors
  1. Yi Zhang
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  2. Chunhui Zhao
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  3. Bing Wu
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  4. Ying Li
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  5. Fang Lv
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  6. Jue Zhang
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  7. Bin Zhou
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  8. Jun Fan
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Correspondence to Biao Huang.

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Zhang, Y., Zhao, C., Wu, B. et al. Rapid and sensitive determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone by biotin–streptavidin-amplified time-resolved fluoroimmunoassay. Fish Sci 84, 715–721 (2018). https://doi.org/10.1007/s12562-018-1211-8

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  • DOI: https://doi.org/10.1007/s12562-018-1211-8

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