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Monitoring the polysaccharide quality of Agaricus blazei in submerged culture by examining molecular weight distribution and TNF-α release capability of macrophage cell line RAW 264.7

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Abstract

A method for monitoring the biological activity of broth polysaccharides of Agaricus blazei (AB-BP) in a submerged culture is described. The TNF-α releasing capability of AB-BP on the macrophage cell line, RAW 264.7, correlated with the molecular weight of AB-BP. The quality of polysaccharide in a submerged culture of Agaricus blazei was indirectly monitored by analyzing the distribution of its molecular weight within 1 h. The harvest time of the maximum polysaccharide production did not coincide with that of the maximum biological characteristics in the batch culture.

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Authors and Affiliations

  1. Department of Chemical and Materials Engineering, National Central University, Chung-Li, Taoyuan, Taiwan, 32054

    Chin-Hang Shu, Bor-Jiun Wen & Ko-Jung Lin

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  1. Chin-Hang Shu
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  2. Bor-Jiun Wen
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  3. Ko-Jung Lin
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Correspondence to Chin-Hang Shu.

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Shu, CH., Wen, BJ. & Lin, KJ. Monitoring the polysaccharide quality of Agaricus blazei in submerged culture by examining molecular weight distribution and TNF-α release capability of macrophage cell line RAW 264.7. Biotechnology Letters 25, 2061–2064 (2003). https://doi.org/10.1023/B:BILE.0000007066.00221.b9

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  • DOI: https://doi.org/10.1023/B:BILE.0000007066.00221.b9

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