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Optimization and Validation in Liquid Chromatography Using Design of Experiments

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Abstract

Faced to the large number of potential factors in liquid chromatography, the analyst is often led to determine through his experience the parameters to be studied. Experimental designs allow the screening of significant factors and the determination of best experimental conditions for analysis. This review mainly focused on the applications of experimental designs in liquid chromatography method development and validation over the last decades. Key advantages are related to the simultaneous control of a large number of variables to achieve desired separation. Several critical issues like multiple response optimization and modeling with least squares are discussed.

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  1. Laboratoire de Chimie Minérale Appliquée, Faculté des Sciences de Tunis, Université de Tunis El Manar, Campus Universitaire El Manar II, 2092, Tunis, Tunisia

    Latifa Latrous

  2. Institut Préparatoire Aux Etudes d’Ingénieurs d’El Manar, Université de Tunis El Manar, El Manar II, B.P.244, 2092, Tunis, Tunisia

    Latifa Latrous

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Latrous, L. Optimization and Validation in Liquid Chromatography Using Design of Experiments. Chemistry Africa 5, 437–458 (2022). https://doi.org/10.1007/s42250-022-00344-1

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