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Preparation and Evaluation of Silicon Quantum Dots-Bonded Silica Stationary Phase for Reversed-Phase Chromatography

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Abstract

In this paper, silicon quantum dots (SiQDs) with green fluorescence are synthesized by solvothermal reaction of 3-(2,3-epoxypropoxy)propyltrimethoxysilane (GPTMS) and ethylenediaminetetraacetic acid (EDTA), and then SiQDs are bonded to the surface of silica to obtain a new nano-on-micro stationary phase (SiO2-SiQDs) for reversed-phase chromatography. The successful preparation of SiO2-SiQDs stationary phase is demonstrated by a variety of characterizations, such as transmission electron microscopy, laser confocal microscopy, elemental analysis and Fourier infrared spectroscopy. In addition, the chromatographic performance of the prepared stationary phase is evaluated and it shows good separation performance for non-polar substances such as alkylbenzene, aniline and polycyclic aromatic hydrocarbons in reversed-phase liquid chromatography. It is also verified that the stationary phase has good methyl selectivity and shape selectivity. More interestingly, the separation of prednisolone and hydrocortisone isomers can also be achieved at a low ratio of organic solvents, indicating that this new stationary phase has a good application prospect in isomer separation.

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The authors confirm that the data supporting the findings of this study are available within this article and its supplementary materials.

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Acknowledgements

This work was financially supported by National Natural Science Foundation of China (22074154), Longyuan Youth Innovation and Entrepreneurship Talent (Team) Project (E20492SC), Lanzhou talent innovation and Entrepreneurship Project (2021-RC-35), Science and Technology Project of Science and Technology Bureau of Chengguan District in Lanzhou City (2020JSCX0033), Youth Innovation Promotion Association CAS (2021420).

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Authors and Affiliations

  1. Laboratory on Pollution Monitoring and Control, College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, 830054, China

    Danni Wang & Hongdeng Qiu

  2. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China

    Danni Wang, Hui Li, Hongdeng Qiu & Jia Chen

  3. College of Chemistry and Chemical Engineering, Gannan Normal University, Ganzhou, 341000, China

    Hongdeng Qiu

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  1. Danni Wang
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  2. Hui Li
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  4. Jia Chen
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Contributions

DW experiment, software, data curation, investigation, writing—original draft. HL software, discussion. HQ Writing—review & editing, discussion, supervision. JC conceptualization, methodology, investigation, writing—review & editing, discussion, supervision. All authors agreed with the final version of the manuscript.

Corresponding authors

Correspondence to Hongdeng Qiu or Jia Chen.

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Wang, D., Li, H., Qiu, H. et al. Preparation and Evaluation of Silicon Quantum Dots-Bonded Silica Stationary Phase for Reversed-Phase Chromatography. J. Anal. Test. 7, 8–15 (2023). https://doi.org/10.1007/s41664-022-00243-x

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  • DOI: https://doi.org/10.1007/s41664-022-00243-x

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