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Graphene Oxide-Mediated Fluorometric Aptasensor for Okadaic Acid Detection

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Abstract

Okadaic acid (OA), which is one of the representative diarrhetic shellfish poisonings, is a prevalent food-poisoning toxin in the world. As OA consumption induces various digestive diseases, a strict monitoring tool for OA is highly required. In this study, we demonstrate the development of an aptamer-based fluorometric OA sensor. First, the affinity of the aptamer towards OA was confirmed through electrochemical impedance spectroscopy, and it was then utilized to develop the fluorometric sensor. The verified OA-specific aptamer was modified with 5-carboxyfluorescein (FAM) as a fluorophore, and graphene oxide (GO) was adopted as the quencher. By virtue of the quenching phenomena between FAM and GO, the developed sensor revealed a low detection limit of 6.35 ppb. Also, this sensor represented a high response to OA compared to other types of marine toxins, such as saxitoxin, domoic acid, and tetrodotoxin.

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Acknowledgements

This research was supported by a grant (20163MFDS641) from Ministry of Food and Drug Safety in 2021 and by the Chung-Ang University Research Scholarship Grants in 2020.

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Authors and Affiliations

  1. Department of Chemistry, Research Institute of Chem-Bio Diagnostic Technology, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06974, Republic of Korea

    So Yeon Kweon, Chan Yeong Park & Tae Jung Park

  2. Department of Food Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea

    Jong Pil Park

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  1. So Yeon Kweon
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  2. Jong Pil Park
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Correspondence to Chan Yeong Park or Tae Jung Park.

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Kweon, S.Y., Park, J.P., Park, C.Y. et al. Graphene Oxide-Mediated Fluorometric Aptasensor for Okadaic Acid Detection. BioChip J 16, 207–213 (2022). https://doi.org/10.1007/s13206-022-00056-1

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  • DOI: https://doi.org/10.1007/s13206-022-00056-1

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