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Exploiting the antibacterial mechanism of phenazine substances from Lysobacter antibioticus 13-6 against Xanthomonas oryzae pv. oryzicola

  • Microbial Pathogenesis and Host-Microbe Interaction
  • Published:
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Abstract

Bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola (Xoc) is one of the most destructive diseases affecting rice production worldwide. In this study, we extracted and purified phenazine substances from the secondary metabolites of Lysobacter antibioticus 13-6. The bacteriostatic mechanism of phenazine substances against Xoc was investigated through physiological response and transcriptomic analysis. Results showed that phenazine substances affects the cell membrane permeability of Xoc, which causes cell swelling and deformation, blockage of flagellum synthesis, and imbalance of intracellular environment. The changes in intracellular environment affect the physiological and metabolic functions of Xoc, which reduces the formation of pathogenic factors and pathogenicity. Through transcriptomic analysis, we found that among differentially expressed genes, the expression of 595 genes was induced significantly (275 up-regulated and 320 down-regulated). In addition, we observed that phenazine substances affects three main functions of Xoc, i.e., transmembrane transporter activity, DNA-mediated transposition, and structural molecular activity. Phenazine substances also inhibits the potassium ion transport system that reduces Xoc resistance and induces the phosphate ion transport system to maintain the stability of the internal environment. Finally, we conclude that phenazine substances could retard cell growth and reduce the pathogenicity of Xoc by affecting cell structure and physiological metabolism. Altogether, our study highlights latest insights into the antibacterial mechanism of phenazine substances against Xoc and provides basic guidance to manage the incidence of bacterial leaf streak of rice.

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Data Availability Statements All raw data related to Transcriptomic analysis (RNA-seq) is submitted to the public database NCBI and is available as Sequence Read Archive (SRA) under accession number PRJNA756441.

Availability of Data and Material This material is the author’s own original work, which has not been previously published elsewhere and has no conflict of interest.

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Acknowledgements

This study was financially supported by the National Key R&D Program of China (2019YFD1002000), the National Natural Science Foundation of China (No. 32060601), and the Yunnan Ten Thousand Talents Plan Leading Talents of Industrial Technology Project of China (YNWR-CYJS-2019-046).

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  1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, P. R. China

    Qi Liu, Jun Yang, Waqar Ahmed, Xiaoyan Wan, Lanfang Wei & Guanghai Ji

  2. Key Laboratory of Agro-Biodiversity and Pest Management of Ministry of Education, Yunnan Agricultural University, Kunming, 650201, P. R. China

    Qi Liu, Jun Yang, Waqar Ahmed, Xiaoyan Wan, Lanfang Wei & Guanghai Ji

  3. College of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong, 675000, P. R. China

    Jun Yang

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GJ and LW conceived and designed the experiments. QL and XW performed the experiments. QL, JY, and WA analyzed the data and made the figures. QL and WA wrote the manuscript. All authors contributed to the final draft of the manuscript.

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Correspondence to Guanghai Ji.

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Liu, Q., Yang, J., Ahmed, W. et al. Exploiting the antibacterial mechanism of phenazine substances from Lysobacter antibioticus 13-6 against Xanthomonas oryzae pv. oryzicola. J Microbiol. 60, 496–510 (2022). https://doi.org/10.1007/s12275-022-1542-0

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