Abstract
Developing sensitive assay for pathogen detection is a sustained demand for food scientists, industries, and government. In this study, an ultrasensitive colorimetric lateral flow assay (LFA) was successfully developed for the detection of Salmonella typhimurium (S. typhimurium). Based on cascade enzyme reaction induced by horseradish peroxidase (HRP)-encapsulated starch-based liposome, the sensitivity of the conventional LFA can be dramatically improved. With this assay, the concentration of Salmonella typhimurium existed in the sample can be efficiently quantified in the range of 102–106 cfu·mL-1. The detection limit (LOD) of the novel assay can be as low as 50 cfu·mL-1 (S/N = 3), appropriate four orders of magnitude lower than the conventional LFA. In addition, the selectivity experiment results indicate this assay has a high selectivity over other commonly encountered pathogens. The practicability of the novel assay was investigated by detecting the concentration of Salmonella typhimurium in spiked real food samples. The average recoveries range from 92.3 to 107.6%, revealing satisfactory application potential of the proposed assay. With the merits of high sensitivity, rapid detection, and no requirement of any additional instruments, we believe that the proposed assay has a promising prospect in the detection of pathogenic bacteria in food.
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Data Availability
The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.
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Funding
This research was supported by the National Key Research & Development Program in China (2019YFD1002704), Shandong major projects of independent innovation (2019JZZY010722), Bohai Sea Granary Science and Technology Demonstration Project (2019BHLC002), Special Project of International Cooperative Research (QLUTGJHZ2018016), Nature Science Foundation of China (31901645), Natural Science Foundation of Shandong Province (ZR2019BC088 and ZR2021QC011), Innovation Pilot Project of Integration of Science, Education and Industry of Shandong Province (Grant No. 2020KJC-ZD06 and No. 2020KJC-ZD011), Innovation Team cultivated by Jinan City (2018GXRC004), National College Student Innovation and Entrepreneurship Training Program and Special Funds for Taishan Scholars Project.
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Ran Yang declares that she has no conflict of interest. Zhen Du declares that he has no conflict of interest. Deyun He declares that she has no conflict of interest. Mingshuang Zhang declares that she has no conflict of interest. Zhengyu Jin declares that he has no conflict of interest. Enbo Xu declares that he has no conflict of interest. Zhengzong Wu declares that he has no conflict of interest. Bo Cui declares that he has no conflict of interest.
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Ran Yang, Zhen Du, and Deyun He contributed equally to the work and should be regarded as co-first authors
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Yang, R., Du, Z., He, D. et al. Equipment-Free Quantitative Detection of Salmonella typhimurium with a Liposome and Enzyme Reaction-Based Lateral Flow Assay. Food Anal. Methods 15, 1482–1489 (2022). https://doi.org/10.1007/s12161-021-02220-z
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DOI: https://doi.org/10.1007/s12161-021-02220-z