Abstract
In this work, the electrochemical determination of antioxidant compounds in green coffee beans using a low-pressure chromatographic system based on a single 1-cm length monolithic column is presented. Screening of the antioxidant compounds in green coffee beans using this approach was performed under different elution conditions, considering different supporting electrolyte media and applied potential values at detection. Mass spectra analyses for the structure elucidation of the separated compounds are presented. Under the selected experimental conditions, five chlorogenic acids (3-, 4-, 5-, cis-5-caffeoylquinic acids, and 5-feruloylquinic acid) and caffeine were identified. Resolution values higher than 1.5 were achieved for all identified compounds. The limits of detection for 5-caffeoylquinic acid and caffeine were 9.25 µmol L−1 and 6.73 µmol L−1, respectively. Acetonitrile consumption was of 0.325 mL per analysis. The figures of merit obtained plus the simplicity of the analytical system assembling and handling highlight the high competitiveness of the presented low-cost approach to be used in substitution of HPLC methodologies currently performed to this end, and for further analytical applications in complex matrices.
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Acknowledgements
The authors are grateful to Dra Zélia Azevedo for her contribution in HPLC-DAD-MS/MS analyses.
Funding
J. R. Santos acknowledges the funding program (DL 57/2016 – Norma transitória) supported by Fundação para a Ciência e a Tecnologia I.P. This work was supported by UID/QUI/50006/2019 with funding from FCT/MCTES through national funds.
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Alexandra Rangel Silva declares no conflict of interest. João Rodrigo Santos declares no conflict of interest. P.J. Almeida declares no conflict of interest. J.A. Rodrigues declares no conflict of interest.
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Rangel Silva, A., Santos, J.R., Almeida, P.J. et al. Screening of Antioxidant Compounds in Green Coffee by Low-Pressure Chromatography with Amperometric Detection. Food Anal. Methods 14, 2175–2185 (2021). https://doi.org/10.1007/s12161-021-02037-w
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DOI: https://doi.org/10.1007/s12161-021-02037-w